The in vitro interaction of nicotine and hemoglobin (Hb) in a metabolizing System was studied by spectroscopy assays. Visible spectra showed two isobestics, and fluorescence spectra showed static quenching with increa...The in vitro interaction of nicotine and hemoglobin (Hb) in a metabolizing System was studied by spectroscopy assays. Visible spectra showed two isobestics, and fluorescence spectra showed static quenching with increasing of nicotine dose. Meanwhile, the CD spectra intensity reduced, showing the conformation of Hb varied markedly through the interaction. All these results suggested that the interaction of nicotine or its metabolites and Hb might do harm to physicological function of Hb.展开更多
DNA adduction with nicotine in mouse liver at environmental doses has been observed using 14C-label by accelerator mass spectrometry(AMS).An improved detection limit of DNA adducts of 1 adduct per 1011 nucleotides has...DNA adduction with nicotine in mouse liver at environmental doses has been observed using 14C-label by accelerator mass spectrometry(AMS).An improved detection limit of DNA adducts of 1 adduct per 1011 nucleotides has been given. It is primarily proposed that nicotine appears carcinogenic via its conversion to nitrosamines.展开更多
基金We thank the financial support of the National Natural Science Foundation of China(Grant.No.19935020)Research Fund for Doctoral Program of Higher Education.
文摘The in vitro interaction of nicotine and hemoglobin (Hb) in a metabolizing System was studied by spectroscopy assays. Visible spectra showed two isobestics, and fluorescence spectra showed static quenching with increasing of nicotine dose. Meanwhile, the CD spectra intensity reduced, showing the conformation of Hb varied markedly through the interaction. All these results suggested that the interaction of nicotine or its metabolites and Hb might do harm to physicological function of Hb.
文摘DNA adduction with nicotine in mouse liver at environmental doses has been observed using 14C-label by accelerator mass spectrometry(AMS).An improved detection limit of DNA adducts of 1 adduct per 1011 nucleotides has been given. It is primarily proposed that nicotine appears carcinogenic via its conversion to nitrosamines.
