Objective:Hereditary colorectal cancer(CRC)accounts for approximately 5%–10%of all CRC cases.The full profile of CRC-related germline mutations and the corresponding somatic mutational profile have not been fully det...Objective:Hereditary colorectal cancer(CRC)accounts for approximately 5%–10%of all CRC cases.The full profile of CRC-related germline mutations and the corresponding somatic mutational profile have not been fully determined in the Chinese population.Methods:We performed the first population study investigating the germline mutation status in more than 1,000(n=1,923)Chinese patients with CRC and examined their relationship with the somatic mutational landscape.Germline alterations were examined with a 58-gene next-generation sequencing panel,and somatic alterations were examined with a 605-gene panel.Results:A total of 92 pathogenic(P)mutations were identified in 85 patients,and 81 likely pathogenic(LP)germline mutations were identified in 62 patients,accounting for 7.6%(147/1,923)of all patients.MSH2 and APC was the most mutated gene in the Lynch syndrome and non-Lynch syndrome groups,respectively.Patients with P/LP mutations had a significantly higher ratio of microsatellite instability,highly deficient mismatch repair,family history of CRC,and lower age.The somatic mutational landscape revealed a significantly higher mutational frequency in the P group and a trend toward higher copy number variations in the non-P group.The Lynch syndrome group had a significantly higher mutational frequency and tumor mutational burden than the nonLynch syndrome group.Clustering analysis revealed that the Notch signaling pathway was uniquely clustered in the Lynch syndrome group,and the MAPK and cAMP signaling pathways were uniquely clustered in the non-Lynch syndrome group.Population risk analysis indicated that the overall odds ratio was 11.13(95%CI:8.289–15.44)for the P group and 20.68(95%CI:12.89–33.18)for the LP group.Conclusions:Distinct features were revealed in Chinese patients with CRC with germline mutations.The Notch signaling pathway was uniquely clustered in the Lynch syndrome group,and the MAPK and cAMP signaling pathways were uniquely clustered in the non-Lynch syndrome group.Patients with P/LP germline mutations exhibited higher CRC risk.展开更多
With the increasing maturity of automatic driving technology,the homogeneous traffic flow will gradually evolve into the heterogeneous traffic flow,which consists of human-driving and autonomous vehicles.To better stu...With the increasing maturity of automatic driving technology,the homogeneous traffic flow will gradually evolve into the heterogeneous traffic flow,which consists of human-driving and autonomous vehicles.To better study the characteristics of the heterogeneous traffic system,this paper proposes a new car-following model for autonomous vehicles and heterogeneous traffic flow,which considers the self-stabilizing effect of vehicles.Through linear and nonlinear methods,this paper deduces and analyzes the stability of such a car-following model with the self-stabilizing effect.Finally,the model is verified by numerical simulation.Numerical results show that the self-stabilizing effect can make the heterogeneous traffic flow more stable,and that increasing the self-stabilizing coefficient or historical time length can strengthen the stability of heterogeneous traffic flow and alleviate traffic congestion effectively.In addition,the heterogeneous traffic flow can also be stabilized with a higher proportion of autonomous vehicles.展开更多
In this study, we investigated the effect of different types of light and MeJA treatment on the accumulation of betulin and oleanolic acid in various organs of white birch. Our results showed that betulin and oleanoli...In this study, we investigated the effect of different types of light and MeJA treatment on the accumulation of betulin and oleanolic acid in various organs of white birch. Our results showed that betulin and oleanolic were accumulated mainly in the stalk skin. The content of both substances in the stalk skin was significantly affected by seasons with a peak accumulation in August. The content of oleanolic and betulin was significantly decreased in the stem skin treated with 4 types of light (red, yellow, blue and green) compared with the plant with normal illumination. In contrast, oleanolic acid in leaves was increased by 13.28 folds when the white birch was treated with green light. Betulin was increased by 1.959 folds in leaves of white birch treated with blue light. The highest content of betulin and oleanolic acid in various organs of birch with appropriate shading treatment (light transmittance: 50%) was increased by 45.09% and 30.50%, respectively, in comparison with those with non-shading treatment. Content of oleanolic acid and betulin can be significantly improved in various parts of birch after treatment with different concentration of MeJA. The study lays the foundation to metabolic regulation of oleanolic acid and betulin in birch.展开更多
Biomarker detection is key to identifying health risks.However,designing sensitive and single-use biosensors for early diagnosis remains a major challenge.Here,we report submonolayer lasers on optical fibers as ultras...Biomarker detection is key to identifying health risks.However,designing sensitive and single-use biosensors for early diagnosis remains a major challenge.Here,we report submonolayer lasers on optical fibers as ultrasensitive and disposable biosensors.Telecom optical fibers serve as distributed optical microcavities with high Q-factor,great repeatability,and ultralow cost,which enables whispering-gallery laser emission to detect biomarkers.It is found that the sensing performance strongly depends on the number of gain molecules.The submonolayer lasers obtained a six-order-of-magnitude improvement in the lower limit of detection(LOD)when compared to saturated monolayer lasers.We further achieve an ultrasensitive immunoassay for a Parkinson's disease biomarker,alpha-synuclein(α-syn),with a lower LOD of 0.32 pM in serum,which is three orders of magnitude lower than theα-syn concentration in the serum of Parkinson's disease patients.Our demonstration of submonolayer biolaser offers great potentials in high-throughput clinical diagnosis with ultimate sensitivity.展开更多
Fiber optofluidic laser(FOFL)integrates optical fiber microcavity and microfluidic channel and provides many unique advantages for sensing applications.FOFLs not only inherit the advantages of lasers such as high sens...Fiber optofluidic laser(FOFL)integrates optical fiber microcavity and microfluidic channel and provides many unique advantages for sensing applications.FOFLs not only inherit the advantages of lasers such as high sensitivity,high signal-to-noise ratio,and narrow linewidth,but also hold the unique features of optical fiber,including ease of integration,high repeatability,and low cost.With the development of new fiber structures and fabrication technologies,FOFLs become an important branch of optical fiber sensors,especially for application in biochemical detection.In this paper,the recent progress on FOFL is reviewed.We focuse mainly on the optical fiber resonators,gain medium,and the emerging sen sing applicatio ns.The prospects for FOFL are also discussed.We believe that the FOFL sensor provides a promising technology for biomedical analysis and environmental monitoring.展开更多
Acousto-optic interactions, employed in the ultrafast laser regulation,possess remarkable advantages for fast tuning performance in a wide spectral range. Here, we propose an ultrafast fiber laser whose wideband tunab...Acousto-optic interactions, employed in the ultrafast laser regulation,possess remarkable advantages for fast tuning performance in a wide spectral range. Here, we propose an ultrafast fiber laser whose wideband tunability is provided by an acousto-optic structure fabricated with an etched single-mode fiber. Because of the laser polarization conversion induced by the coupling between the core and cladding vector modes in the etched fiber,a band-pass characteristic of the acousto-optic interaction is achieved to effectively regulate the inner-cavity gain range. Cooperating with a saturable absorber based on single-wall carbon nanotubes (SWCNTs) with polarization robustness,a soliton operating state is achieved in the tunable erbium-doped fiber laser.By controlling the acoustical wave frequency from 1.039 to 1.069 MHz, this soliton laser can be conveniently tuned in a wide spectral range from 1571.52 to 1539.26nm.Meanwhile, the laser pulses have near-transform-limited durations stably maintaining less than 2 ps at different wavelength channels,owing to the broadband nonlinear absorption of SWCNTs.展开更多
Currently,as a basic task of military document information extraction,Named Entity Recognition(NER)for military documents has received great attention.In 2020,China Conference on Knowledge Graph and Semantic Computing...Currently,as a basic task of military document information extraction,Named Entity Recognition(NER)for military documents has received great attention.In 2020,China Conference on Knowledge Graph and Semantic Computing(CCKS)and System Engineering Research Institute of Academy of Military Sciences(AMS)issued the NER task for test evaluation,which requires the recognition of four types of entities including Test Elements(TE),Performance Indicators(PI),System Components(SC)and Task Scenarios(TS).Due to the particularity and confidentiality of the military field,only 400 items of annotated data are provided by the organizer.In this paper,the task is regarded as a few-shot learning problem for NER,and a method based on BERT and two-level model fusion is proposed.Firstly,the proposed method is based on several basic models fine tuned by BERT on the training data.Then,a two-level fusion strategy applied to the prediction results of multiple basic models is proposed to alleviate the over-fitting problem.Finally,the labeling errors are eliminated by post-processing.This method achieves F1 score of 0.7203 on the test set of the evaluation task.展开更多
基金This study was supported by the Special Funds for Strategic Emerging Industry Development of Shenzhen(Grant No.20170922151538732)the Science and Technology Project of Shenzhen(Grant No.JSGG20180703164202084)+2 种基金the Natural Science Foundation Project of China(Grant No.71573022)the National Natural Science Foundation Regional Projects(Grant No.82060440)the special health research projects of 2019 funded by the Chinese PLA General Hospital(Grant No.NLBJ-2019003).
文摘Objective:Hereditary colorectal cancer(CRC)accounts for approximately 5%–10%of all CRC cases.The full profile of CRC-related germline mutations and the corresponding somatic mutational profile have not been fully determined in the Chinese population.Methods:We performed the first population study investigating the germline mutation status in more than 1,000(n=1,923)Chinese patients with CRC and examined their relationship with the somatic mutational landscape.Germline alterations were examined with a 58-gene next-generation sequencing panel,and somatic alterations were examined with a 605-gene panel.Results:A total of 92 pathogenic(P)mutations were identified in 85 patients,and 81 likely pathogenic(LP)germline mutations were identified in 62 patients,accounting for 7.6%(147/1,923)of all patients.MSH2 and APC was the most mutated gene in the Lynch syndrome and non-Lynch syndrome groups,respectively.Patients with P/LP mutations had a significantly higher ratio of microsatellite instability,highly deficient mismatch repair,family history of CRC,and lower age.The somatic mutational landscape revealed a significantly higher mutational frequency in the P group and a trend toward higher copy number variations in the non-P group.The Lynch syndrome group had a significantly higher mutational frequency and tumor mutational burden than the nonLynch syndrome group.Clustering analysis revealed that the Notch signaling pathway was uniquely clustered in the Lynch syndrome group,and the MAPK and cAMP signaling pathways were uniquely clustered in the non-Lynch syndrome group.Population risk analysis indicated that the overall odds ratio was 11.13(95%CI:8.289–15.44)for the P group and 20.68(95%CI:12.89–33.18)for the LP group.Conclusions:Distinct features were revealed in Chinese patients with CRC with germline mutations.The Notch signaling pathway was uniquely clustered in the Lynch syndrome group,and the MAPK and cAMP signaling pathways were uniquely clustered in the non-Lynch syndrome group.Patients with P/LP germline mutations exhibited higher CRC risk.
基金supported by the National Natural Science Foundation of China(Grant No.61773243)the Major Technology Innovation Project of Shandong Province,China(Grant No.2019TSLH0203)the National Key Research and Development Program of China(Grant No.2020YFB1600501)。
文摘With the increasing maturity of automatic driving technology,the homogeneous traffic flow will gradually evolve into the heterogeneous traffic flow,which consists of human-driving and autonomous vehicles.To better study the characteristics of the heterogeneous traffic system,this paper proposes a new car-following model for autonomous vehicles and heterogeneous traffic flow,which considers the self-stabilizing effect of vehicles.Through linear and nonlinear methods,this paper deduces and analyzes the stability of such a car-following model with the self-stabilizing effect.Finally,the model is verified by numerical simulation.Numerical results show that the self-stabilizing effect can make the heterogeneous traffic flow more stable,and that increasing the self-stabilizing coefficient or historical time length can strengthen the stability of heterogeneous traffic flow and alleviate traffic congestion effectively.In addition,the heterogeneous traffic flow can also be stabilized with a higher proportion of autonomous vehicles.
文摘In this study, we investigated the effect of different types of light and MeJA treatment on the accumulation of betulin and oleanolic acid in various organs of white birch. Our results showed that betulin and oleanolic were accumulated mainly in the stalk skin. The content of both substances in the stalk skin was significantly affected by seasons with a peak accumulation in August. The content of oleanolic and betulin was significantly decreased in the stem skin treated with 4 types of light (red, yellow, blue and green) compared with the plant with normal illumination. In contrast, oleanolic acid in leaves was increased by 13.28 folds when the white birch was treated with green light. Betulin was increased by 1.959 folds in leaves of white birch treated with blue light. The highest content of betulin and oleanolic acid in various organs of birch with appropriate shading treatment (light transmittance: 50%) was increased by 45.09% and 30.50%, respectively, in comparison with those with non-shading treatment. Content of oleanolic acid and betulin can be significantly improved in various parts of birch after treatment with different concentration of MeJA. The study lays the foundation to metabolic regulation of oleanolic acid and betulin in birch.
基金This work is supported by the National Natural Science Foundation of China(Grant Nos.62275043,61875034,62205007,11825402,and 62105006)the 111 Project(B14039)+4 种基金the Sichuan Science and Technology Program(2021YJ0101)the Fundamental Research Funds for the Central Universities(ZYGX2021YGCX007)the China Postdoctoral Science Foundation(Grant Nos.2021T1400232020M680187,and 2021M700208)the Young Elite Scientists Sponsorship Program by CAST(2022QNRC001)。
文摘Biomarker detection is key to identifying health risks.However,designing sensitive and single-use biosensors for early diagnosis remains a major challenge.Here,we report submonolayer lasers on optical fibers as ultrasensitive and disposable biosensors.Telecom optical fibers serve as distributed optical microcavities with high Q-factor,great repeatability,and ultralow cost,which enables whispering-gallery laser emission to detect biomarkers.It is found that the sensing performance strongly depends on the number of gain molecules.The submonolayer lasers obtained a six-order-of-magnitude improvement in the lower limit of detection(LOD)when compared to saturated monolayer lasers.We further achieve an ultrasensitive immunoassay for a Parkinson's disease biomarker,alpha-synuclein(α-syn),with a lower LOD of 0.32 pM in serum,which is three orders of magnitude lower than theα-syn concentration in the serum of Parkinson's disease patients.Our demonstration of submonolayer biolaser offers great potentials in high-throughput clinical diagnosis with ultimate sensitivity.
基金This work is supported by the National Natural Science Foundation of China （61107073, 61107072, and 61290312）, Fundamental Research Funds for the Central Universities （ZYGX2011J002）, Research Fund for the Doctoral Program of Higher Education of China （20110185120020）, Program for Changjiang Scholars and Innovative Research Team in University （PCSIRT, IRT1218）, and the 111 Project （B 14039）.
基金the financial support from the National Natural Science Foundation of China(Grant No.61875034)the 111 Project(Grant No.B14039)Seeding Project of Scientific and Technical Innovation of Sichuan Province(Grant No.2020107).
文摘Fiber optofluidic laser(FOFL)integrates optical fiber microcavity and microfluidic channel and provides many unique advantages for sensing applications.FOFLs not only inherit the advantages of lasers such as high sensitivity,high signal-to-noise ratio,and narrow linewidth,but also hold the unique features of optical fiber,including ease of integration,high repeatability,and low cost.With the development of new fiber structures and fabrication technologies,FOFLs become an important branch of optical fiber sensors,especially for application in biochemical detection.In this paper,the recent progress on FOFL is reviewed.We focuse mainly on the optical fiber resonators,gain medium,and the emerging sen sing applicatio ns.The prospects for FOFL are also discussed.We believe that the FOFL sensor provides a promising technology for biomedical analysis and environmental monitoring.
基金This work is supported by the National Natural Science Foundation of China （61107073, 61107072 and 61290312）, Fundamental Research Funds for the Central Universities （ZYGX2011J002）, Research Fund for the Doctoral Program of Higher Education of China （20110185120020）, Program for Changjiang Scholars and Innovative Research Team in University （PCSIRT, IRT1218）, and the 111 Project （B 14039）. Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author（s） and source are credited.
基金National Natural Science Foundation of China(NSFC)(61635004,61705023,61705024)Key Research and Development Project of Ministry of Science and Technology(2016YFC0801200)+4 种基金Chongqing Postdoctoral Program for Innovative Talents(CQBX201703)Postdoctoral Science Foundation of Chongqing(Xm2017047)Natural Science Foundation of Chongqing(cstc2018jcyjAX0644)Science and Technology on Plasma Physics Laboratory(6142A0403050817)National Science Fund for Distinguished Young Scholars(61825501)
文摘Acousto-optic interactions, employed in the ultrafast laser regulation,possess remarkable advantages for fast tuning performance in a wide spectral range. Here, we propose an ultrafast fiber laser whose wideband tunability is provided by an acousto-optic structure fabricated with an etched single-mode fiber. Because of the laser polarization conversion induced by the coupling between the core and cladding vector modes in the etched fiber,a band-pass characteristic of the acousto-optic interaction is achieved to effectively regulate the inner-cavity gain range. Cooperating with a saturable absorber based on single-wall carbon nanotubes (SWCNTs) with polarization robustness,a soliton operating state is achieved in the tunable erbium-doped fiber laser.By controlling the acoustical wave frequency from 1.039 to 1.069 MHz, this soliton laser can be conveniently tuned in a wide spectral range from 1571.52 to 1539.26nm.Meanwhile, the laser pulses have near-transform-limited durations stably maintaining less than 2 ps at different wavelength channels,owing to the broadband nonlinear absorption of SWCNTs.
文摘Currently,as a basic task of military document information extraction,Named Entity Recognition(NER)for military documents has received great attention.In 2020,China Conference on Knowledge Graph and Semantic Computing(CCKS)and System Engineering Research Institute of Academy of Military Sciences(AMS)issued the NER task for test evaluation,which requires the recognition of four types of entities including Test Elements(TE),Performance Indicators(PI),System Components(SC)and Task Scenarios(TS).Due to the particularity and confidentiality of the military field,only 400 items of annotated data are provided by the organizer.In this paper,the task is regarded as a few-shot learning problem for NER,and a method based on BERT and two-level model fusion is proposed.Firstly,the proposed method is based on several basic models fine tuned by BERT on the training data.Then,a two-level fusion strategy applied to the prediction results of multiple basic models is proposed to alleviate the over-fitting problem.Finally,the labeling errors are eliminated by post-processing.This method achieves F1 score of 0.7203 on the test set of the evaluation task.
