A novel immunoassay method for methyltestosterone (MT) in serum ovas developed.The antigen of MT (Ag(MT))was synthesized by covalently bonding MT to BSA and raising its antibody (Ab(MT)). Ab(MT) was con.jugated to pol...A novel immunoassay method for methyltestosterone (MT) in serum ovas developed.The antigen of MT (Ag(MT))was synthesized by covalently bonding MT to BSA and raising its antibody (Ab(MT)). Ab(MT) was con.jugated to poly-N-isopropylacrylamide (p-NIPAAm) to form Ab(MT) bound thermally reversible hydrogel p-NIPAAm-Ab((MT) A competitive immunoassay method based on the competition of fluorescein isothiocyanate (FITC) labeled MT antigen (FITC-Ag(MT)) and free MT with limited amount of p-NIPAAm-Ab(MT) was established. The separation in the assay process was achieved by precipitation of the immuno-complex above its critical solution temperature. The detection limit for MT is 50ng/ml. The recoveries of MT from human serum are satisfactory.展开更多
The relationship between meltillg point and acid denaturing acidity of DNA was studied by ultraviolet spectrometer. According to our investigation there exists a good linear relationship between melting point and acid...The relationship between meltillg point and acid denaturing acidity of DNA was studied by ultraviolet spectrometer. According to our investigation there exists a good linear relationship between melting point and acid denaturing acidity of DNA. The acid denaturing acidity. which could be obtained easily. may be used in the field of biochemistry as an alternative to melting point.展开更多
文摘A novel immunoassay method for methyltestosterone (MT) in serum ovas developed.The antigen of MT (Ag(MT))was synthesized by covalently bonding MT to BSA and raising its antibody (Ab(MT)). Ab(MT) was con.jugated to poly-N-isopropylacrylamide (p-NIPAAm) to form Ab(MT) bound thermally reversible hydrogel p-NIPAAm-Ab((MT) A competitive immunoassay method based on the competition of fluorescein isothiocyanate (FITC) labeled MT antigen (FITC-Ag(MT)) and free MT with limited amount of p-NIPAAm-Ab(MT) was established. The separation in the assay process was achieved by precipitation of the immuno-complex above its critical solution temperature. The detection limit for MT is 50ng/ml. The recoveries of MT from human serum are satisfactory.
文摘The relationship between meltillg point and acid denaturing acidity of DNA was studied by ultraviolet spectrometer. According to our investigation there exists a good linear relationship between melting point and acid denaturing acidity of DNA. The acid denaturing acidity. which could be obtained easily. may be used in the field of biochemistry as an alternative to melting point.
