A new rapid and accurate method for determining inosine by capillary zone electrophoresis(CZE) is presented. The electrophoresis separation was performed at 24℃ in a fused-silica capillary(51.2cm×50 μmi.d., 38...A new rapid and accurate method for determining inosine by capillary zone electrophoresis(CZE) is presented. The electrophoresis separation was performed at 24℃ in a fused-silica capillary(51.2cm×50 μmi.d., 38.5cm to UV detector)using 20mmol/L borax-20mmol/L H3PO4(pH 8.5) as the background electrolyte at 24KV applied voltage. Inosine samples were led into the capillary electrokinetically at 10KV for 15 seconds. In the range of 20μg/mL to 300μg/mL, inosine can be quantitatively determined with a deduction limit of 2.1μg/mL and a RSD of 2.92%. Hypoxanthine was used as the internal standard.展开更多
文摘A new rapid and accurate method for determining inosine by capillary zone electrophoresis(CZE) is presented. The electrophoresis separation was performed at 24℃ in a fused-silica capillary(51.2cm×50 μmi.d., 38.5cm to UV detector)using 20mmol/L borax-20mmol/L H3PO4(pH 8.5) as the background electrolyte at 24KV applied voltage. Inosine samples were led into the capillary electrokinetically at 10KV for 15 seconds. In the range of 20μg/mL to 300μg/mL, inosine can be quantitatively determined with a deduction limit of 2.1μg/mL and a RSD of 2.92%. Hypoxanthine was used as the internal standard.
