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Molecular characteristics of Brucella melitensis isolates from humans in Qinghai Province, China 被引量:5
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作者 Zhi-Jun zhao Ji-Quan Li +12 位作者 Li Ma Hong-Mei Xue Xu-Xin Yang yuan-bo zhao Yu-Min Qin Xiao-Wen Yang Dong-Ri Piao Hong-Yan zhao Guo-Zhong Tian Qiang LiJian-Ling Wang Guang Tian Hai Jiang Li-Qing Xu 《Infectious Diseases of Poverty》 SCIE 2021年第2期64-69,共6页
Background:The prevalence of human brucellosis in Qinghai Province of China has been increasing rapidly,with confirmed cases distributed across 31 counties.However,the epidemiology of brucellosis transmission has not ... Background:The prevalence of human brucellosis in Qinghai Province of China has been increasing rapidly,with confirmed cases distributed across 31 counties.However,the epidemiology of brucellosis transmission has not been fully elucidated.To characterize the infecting strains isolated from humans,multiple-locus variable-number tandem repeats analysis(MLVA)and whole-genome single-nucleotide polymorphism(SNP)-based approaches were employed.Methods:Strains were isolated from two males blood cultures that were confirmed Brucella m elitensis positive following biotyping and MLVA.Genomic DNA was extracted from these two strains,and whole-genome sequencing was performed.Next,SNP-based phylogenetic analysis was performed to compare the two strains to 94 B.melitensis strains(complete genome and draft genome)retrieved from online databases.Results:The two Brucella isolates were identified as B.melitensis biovar 3(QH2019001 and QH2019005)following conventional biotyping and were found to have differences in their variable number tandem repeats(VNTRs)using MLVA-16.Phylogenetic examination assigned the 96 strains to five genotype groups,with QH2019001 and QH2019005 assigned to the same group,but different subgroups.Moreover,the QH2019005 strain was assigned to a new subgenotype,llj,within genotype II.These findings were then combined to determine the geographic origin of the two Brucella strains.Conclusions:Utilizing a whole-genome SNP-based approach enabled differences between the two B.m elitensis strains to be more clearly resolved,and facilitated the elucidation of their different evolutionary histories.This approach also revealed that QH2019005 is a member of a new subgenotype(Hj)with an ancient origin in the eastern Mediterranean Sea. 展开更多
关键词 Brucella m elitensis Multiple-locus variable-number tandem repeats analysis Whole-genome sequencing Single-nucleotide polymorphism
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