Vitellogenin accumulation leads to reproductive senescence by impairing lysosomal function

The maintenance of proteostasis is essential for cellular and organism healthspan.How proteostasis collapse influences reproductive span remains largely unclear.In Caenorhabditis elegans,excess accumulation of vitellogenins,the major components in yolk proteins,is crucial for the development of the embryo and occurs throughout the whole body during the aging process.Here,we show that vitellogenin accumulation leads to reproduction cessation.Excess vitellogenin is accumulated in the intestine and transported into the germline,impairing lysosomal activity in these tissues.The lysosomal function in the germline is required for reproductive span by maintaining oocyte quality.In contrast,autophagy and sperm depletion are not involved in vitellogenin accumulation-induced reproductive aging.Our findings provide insights into how proteome imbalance has an impact on reproductive aging and imply that improvement of lysosomal function is an effective approach for mid-life intervention for maintaining reproductive health in mammals.

A retrospective study to compare the treatment outcomes with and without surgical navigation for fracture of the orbital wall

Purpose To evaluate the outcomes with and without aid of a computer-assisted surgical navigation system(CASNS)for treatment of unilateral orbital wall fracture(OWF).Methods Patients who came to our hospital for repairing unilateral traumatic OWF from 2014 to 2017 were included in this study.The patients were divided into the navigation group who accepted orbital wall reconstruction aided by CASNS and the conventional group.We evaluated the surgical precision in the navigation group by analyzing the difference between actual postoperative computed tomography data and preoperative virtual surgical plan through color order ratios.We also compared the duration of surgery,enophthalmos correction,restoration of orbital volumes,and improvement of clinical symptoms in both groups systemically.Quantitative data were presented as mean±SD.Significance was determined by the two-sample t-test using SPSS Version 19.0 A p<0.05 was considered statistically significant.Results Seventy patients with unilateral OWF were included in the study cohort.The mean difference between preoperative virtual planning and actual reconstruction outcome was(0.869±0.472)mm,which means the reconstruction result could match the navigation planning accurately.The mean duration of surgery in the navigation group was shorter than it is in the control group,but not significantly.Discrepancies between the reconstructed and unaffected orbital-cavity volume and eyeball projection in the navigation group were significantly less than that in the conventional group.One patient had remnant diplopia and two patients had enophthalmos after surgery in the navigation group;two patients had postoperative diplopia and four patients had postoperative enophthalmos in the conventional group.Conclusion Compare with the conventional treatment for OWF,the use of CASNS can provide a significantly better surgical precision,greater improvements in orbital-cavity volume and eyeball projection,and better clinical results,without increasing the duration of surgery.

High Spicy Food Intake and Risk of Cancer： A Meta-analysis of Case-control Studies

Background：Studies on the association between spicy food intake and cancer risk have reported inconsistent results.We quantitatively assessed this association by conducting a meta-analysis based on evidence from case-control studies.Methods：PubMed,EMBASE,and the Cochrane Library were searched for eligible publications.Combined odds ratios （ORs） with their 95% confidence interval （CI） were calculated using a random-or fixed-effects model.The methodological quality of the included articles was assessed using the Newcastle-Ottawa scale （NOS）.All data were analyzed using STATA 1 1.0 software （version 11.0;StataCorp.,College Station,TX,USA）.Subgroup analyses were also performed with stratification by region,sex,number of cases,cancer subtype,source of the control group,and NOS score.Results：A total 39 studies from 28 articles fulfilled the inclusion criteria for the meta-analysis （7884 patients with cancer and 10,142 controls）.Comparison of the highest versus lowest exposure category in each study revealed a significant OR of 1.76 （95% CI =1.35-2.29） in spite of significant heterogeneity （P 〈 0.00l）.In the subgroup analyses,this positive correlation was still found for gastric cancer,different regions,different numbers of cases,different sources of the control group,and high-quality articles （NOS score of≥ 7）.However,no statistically significant association was observed for women,esophageal cancer,gallbladder cancer,or low-quality articles （NOS score of〈7）.No evidence of publication bias was found.Conclusions：Evidence from case-control studies suggested that a higher level of spicy food intake may be associated with an increased incidence of cancer despite significant heterogeneity.More studies are warranted to clarify our understanding of the association between high spicy food intake and the risk of cancer.

Comparison of patients hospitalized with COVID-19,H7N9 and H1N1

Background: There is an urgent need to better understand the novel coronavirus,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),for that the coronavirus disease 2019(COVID-19)continues to cause considerable morbidity and mortality worldwide.This paper was to differentiate COVID-19 from other respiratory infectious diseases such as avian-origin influenza A(H7N9)and influenza A(H1N1)virus infections.Methods:: We included patients who had been hospitalized with laboratory-confirmed infection by SARS-CoV-2(n=83),H7N9(n=36),H1N1(n=44)viruses.Clinical presentation,chest CT features,and progression of patients were compared.We used the Logistic regression model to explore the possible risk factors.Results: Both COVID-19 and H7N9 patients had a longer duration of hospitalization than H1N1 patients(P<0.01),a higher complication rate,and more severe cases than H1N1 patients.H7N9 patients had higher hospitalization-fatality ratio than COVID-19 patients(P=0.01).H7N9 patients had similar patterns of lymphopenia,neutrophilia,elevated alanine aminotransferase,C-reactive protein,lactate dehydrogenase,and those seen in H1N1 patients,which were all significantly different from patients with COVID-19(P<0.01).Either H7N9 or H1N1 patients had more obvious symptoms,like fever,fatigue,yellow sputum,and myalgia than COVID-19 patients(P<0.01).The mean duration of viral shedding was 9.5 days for SARS-CoV-2 vs 9.9 days for H7N9(P=0.78).For severe cases,the meantime from illness onset to severity was 8.0 days for COVID-19 vs 5.2 days for H7N9(P<0.01),the comorbidity of chronic heart disease was more common in the COVID-19 patients than H7N9(P=0.02).Multivariate analysis showed that chronic heart disease was a possible risk factor(OR>1)for COVID-19,compared with H1N1 and H7N9.Conclusions: The proportion of severe cases were higher for H7N9 and SARS-CoV-2 infections,compared with H1N1.The meantime from illness onset to severity was shorter for H7N9.Chronic heart disease was a possible risk factor for COVID-19.The comparison may provide the rationale for strategies of isolation and treatment of infected patients in the future.