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问号钩端螺旋体感染诱导人巨噬细胞发生自噬及自噬作用研究
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作者 谷媛媛 谢玲 +2 位作者 唐东 谭燕 陈旭 《中国人兽共患病学报》 CSCD 北大核心 2024年第12期1107-1114,共8页
目的了解问号钩端螺旋体(简称钩体)对人巨噬细胞自噬的影响,并研究自噬在巨噬细胞杀灭钩体过程中的作用。方法人THP-1单核细胞用佛波酯(PMA)预处理使其分化为巨噬细胞。采用透射电镜法检测细胞内钩体及自噬小体形态。采用激光共聚焦显... 目的了解问号钩端螺旋体(简称钩体)对人巨噬细胞自噬的影响,并研究自噬在巨噬细胞杀灭钩体过程中的作用。方法人THP-1单核细胞用佛波酯(PMA)预处理使其分化为巨噬细胞。采用透射电镜法检测细胞内钩体及自噬小体形态。采用激光共聚焦显微镜法结合LC3-II-GFP质粒脂质体转染技术检测胞内自噬小体形成情况及与钩体共定位水平。采用Western blotting技术检测自噬相关蛋白LC3和P62表达水平。采用激光共聚焦显微镜技术检测自噬小体与溶酶体共定位情况及胞内问号钩体活力。结果钩体感染巨噬细胞后,胞内出现包裹有钩体的自噬小体,胞内LC3-II-GFP聚集点的数量增加,LC3-II-GFP与钩体共定位水平增加,且自噬小体与溶酶体共定位率增加。Western blotting检测结果显示,钩体感染巨噬细胞后,胞内LC3-II蛋白表达量增加,P62蛋白表达水平降低。自噬激活后细胞内问号钩体死亡数量增加,反之亦然。结论问号钩体感染可诱导人巨噬细胞产生自噬,且自噬有助于人巨噬细胞杀灭问号钩体。 展开更多
关键词 钩端螺旋体病 问号钩端螺旋体 感染 人巨噬细胞 自噬
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Review on prevention of falls in hospital settings 被引量:2
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作者 yuan-yuan gu Koen Balcaen +2 位作者 Yicheng Ni Jan Ampe Jan Goffin 《Chinese Nursing Research》 CAS 2016年第1期7-10,共4页
This review will first cover the root causes of falls, identify preventive measures associated with these falls, and provide an overview of best practice of fall prevention at leading institutions. There is signif- ic... This review will first cover the root causes of falls, identify preventive measures associated with these falls, and provide an overview of best practice of fall prevention at leading institutions. There is signif- icant benefit in instituting a comprehensive program to reduce falls. After analyzing the results from many successful programs, it is apparent that an integrative program that consists of patient evaluations, environmental modification, and staff training can lead to a significant reduction in the overall preva- lence of falls. Such programs can be implemented at a low cost and therefore represent an improvement in care with a high return on investment. 展开更多
关键词 Hospital settingsFallFall preventionIntervention
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