Potential rat model of anxiety-like gastric hypersensitivity induced by sequential stress

AIM To establish a rat model of anxiety-like gastric hyper-sensitivity(GHS) of functional dyspepsia(FD) induced by novel sequential stress.METHODS Animal pups were divided into two groups from postnatal day 2: controls and the sequential-stress-treated. The sequential-stress-treated group received maternal separation and acute gastric irritation early in life and restraint stress in adulthood; controls were reared undisturbed with their mothers. Rats in both groups were followed to adulthood(8 wk) at which point the anxietylike behaviors and visceromotor responses to gastric distention(20-100 mm Hg) and gastric emptying were tested. Meanwhile, alterations in several anxiety-related brain-stomach modulators including 5-hydroxytryptamine(5-HT), γ-aminobutyric acid(GABA), brain-derived neurotrophic factor(BDNF) and nesfatin-1 in the rat hippocampus, plasma and gastric fundus and the 5-HT1 A receptor(5-HT1 AR) in the hippocampal CA1 subfield and the mucosa of the gastric fundus were examined.RESULTS Sequential-stress-treated rats simultaneously demonstrated anxiety-like behaviors and GHS in dose-dependent manner compared with the control group. Although rats in both groups consumed similar amount of solid food, the rate of gastric emptying was lower in the sequentialstress-treated rats than in the control group. Sequential stress significantly decreased the levels of 5-HT(51.91 ± 1.88 vs 104.21 ± 2.88, P < 0.01), GABA(2.38 ± 0.16 vs 5.01 ± 0.13, P < 0.01) and BDNF(304.40 ± 10.16 vs 698.17 ± 27.91, P < 0.01) in the hippocampus but increased the content of nesfatin-1(1961.38 ± 56.89 vs 1007.50 ± 33.05, P < 0.01) in the same site; significantly decreased the levels of 5-HT(47.82 ± 2.29 vs 89.45 ± 2.61, P < 0.01) and BDNF(257.05 ± 12.89 vs 536.71 ± 20.73, P < 0.01) in the plasma but increased the content of nesfatin-1 in it(1391.75 ± 42.77 vs 737.88 ± 33.15, P < 0.01); significantly decreased the levels of 5-HT(41.15 ± 1.81 vs 89.17 ± 2.31, P < 0.01) and BDNF(226.49 ± 12.10 vs 551.36 ± 16.47, P < 0.01) in the gastric fundus but increased the content of nesfatin-1 in the same site(1534.75 ± 38.52 vs 819.63 ± 38.04, P < 0.01). The expressions of 5-HT1 AR in the hippocampal CA1 subfield and the mucosa of the gastric fundus were down-regulated measured by IHC(Optical Density value: Hippocampus 15253.50 ± 760.35 vs 21149.75 ± 834.13; gastric fundus 15865.25 ± 521.24 vs 23865.75 ± 1868.60; P < 0.05, respectively) and WB(0.38 ± 0.01 vs 0.57 ± 0.03, P < 0.01)(n = 8 in each group). CONCLUSION Sequential stress could induce a potential rat model of anxiety-like GHS of FD, which could be used to research the mechanisms of this intractable disease.

24小时食管多通道腔内阻抗-pH监测阳性的113例胃食管反流病(GERD)患者的胃食管反流特点（英文）

目的:通过分析多通道腔内阻抗-pH监测(MII-pH)阳性的胃食管反流病(GERD)患者的各项反流指标,探讨我国GERD患者的胃食管反流特点,以及性别、年龄、体位和体质指数(BMI)对反流的影响。创新点:入组患者均经MII-pH监测证实具有客观存在的胃食管反流,分析体位和BMI对反流的影响,并且发现我国GERD患者普遍存在弱酸反流。方法:有典型反流症状的疑诊GERD患者,进行24 h MII-pH监测,对监测结果阳性的患者纳入研究对象,分析其各反流指标以及性别、年龄、体位和BMI对反流的影响。结论:我国GERD患者普遍存在弱酸反流,男性、直立体位、肥胖(BMI≥25)可促进胃食管反流的发生,且年龄不是胃食管反流的危险因素。

短节段Barrett’s食管的胃食管酸反流与餐后近端胃酸袋的相关性研究

目的:检测并分析短节段Barrett’s食管(BE)患者的餐后近端胃酸袋(PPGAP)的特点,并分析其与胃食管酸反流的相关性。创新点:PPGAP可能是胃食管反流病的病理因素之一,引起广泛关注,而BE患者的PPGAP特点及与反流的相关性尚无报道。方法:15名短节段BE患者和15名健康志愿者分别以牵拉法检测PPGAP的发生时间、长度、最低pH值和平均pH值。BE患者完成Gerd Q问卷调查和24 h食管pH监测。结论:短节段BE患者普遍存在PPGAP,同时PPGAP的长度和酸度与其胃食管酸反流周期数、De Meester评分和Gerd Q评分具有相关性。