Objective Genomic alterations and potential neoantigens for cervical cancer immunotherapy were identified in a cohort of Chinese patients with cervical squamous cell carcinoma(CSCC).Methods Whole-exome sequencing was ...Objective Genomic alterations and potential neoantigens for cervical cancer immunotherapy were identified in a cohort of Chinese patients with cervical squamous cell carcinoma(CSCC).Methods Whole-exome sequencing was used to identify genomic alterations and potential neoantigens for CSCC immunotherapy.RNA Sequencing was performed to analyze neoantigen expression.Results Systematic bioinformatics analysis showed that C>T/G>A transitions/transversions were dominant in CSCCs.Missense mutations were the most frequent types of somatic mutation in the coding sequence regions.Mutational signature analysis detected signature 2,signature 6,and signature 7 in CSCC samples.PIK3CA,FBXW7,and BICRA were identified as potential driver genes,with BICRA as a newly reported gene.Genomic variation profiling identified 4,960 potential neoantigens,of which 114 were listed in two neoantigen-related databases.Conclusion The present findings contribute to our understanding of the genomic characteristics of CSCC and provide a foundation for the development of new biotechnology methods for individualized immunotherapy in CSCC.展开更多
With the development of artificial intelligence(AI)technologies,biomedical imaging data play an important role in scientific research and clinical application,but the available resources are limited.Here we present Op...With the development of artificial intelligence(AI)technologies,biomedical imaging data play an important role in scientific research and clinical application,but the available resources are limited.Here we present Open Biomedical Imaging Archive(OBIA),a repository for archiving biomedical imaging and related clinical data.OBIA adopts five data objects(Collection,Individual,Study,Series,and Image)for data organization,and accepts the submission of biomedical images of multiple modalities,organs,and diseases.In order to protect personal privacy,OBIA has formulated a unified de-identification and quality control process.In addition,OBIA provides friendly and intuitive web interfaces for data submission,browsing,and retrieval,as well as image retrieval.As of September 2023,OBIA has housed data for a total of 937 individuals,4136 studies,24,701 series,and 1,938,309 images covering 9 modalities and 30 anatomical sites.Collectively,OBIA provides a reliable platform for biomedical imaging data management and offers free open access to all publicly available data to support research activities throughout the world.OBIA can be accessed at https://ngdc.cncb.ac.cn/obia.展开更多
文摘Objective Genomic alterations and potential neoantigens for cervical cancer immunotherapy were identified in a cohort of Chinese patients with cervical squamous cell carcinoma(CSCC).Methods Whole-exome sequencing was used to identify genomic alterations and potential neoantigens for CSCC immunotherapy.RNA Sequencing was performed to analyze neoantigen expression.Results Systematic bioinformatics analysis showed that C>T/G>A transitions/transversions were dominant in CSCCs.Missense mutations were the most frequent types of somatic mutation in the coding sequence regions.Mutational signature analysis detected signature 2,signature 6,and signature 7 in CSCC samples.PIK3CA,FBXW7,and BICRA were identified as potential driver genes,with BICRA as a newly reported gene.Genomic variation profiling identified 4,960 potential neoantigens,of which 114 were listed in two neoantigen-related databases.Conclusion The present findings contribute to our understanding of the genomic characteristics of CSCC and provide a foundation for the development of new biotechnology methods for individualized immunotherapy in CSCC.
基金supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB38050300)the Genomics Data Center Operation and Maintenance of Chinese Academy of Sciences(Grant No.CAS-WX2022SDC-XK05)the Key Technology Talent Program of the Chinese Academy of Sciences,China.
文摘With the development of artificial intelligence(AI)technologies,biomedical imaging data play an important role in scientific research and clinical application,but the available resources are limited.Here we present Open Biomedical Imaging Archive(OBIA),a repository for archiving biomedical imaging and related clinical data.OBIA adopts five data objects(Collection,Individual,Study,Series,and Image)for data organization,and accepts the submission of biomedical images of multiple modalities,organs,and diseases.In order to protect personal privacy,OBIA has formulated a unified de-identification and quality control process.In addition,OBIA provides friendly and intuitive web interfaces for data submission,browsing,and retrieval,as well as image retrieval.As of September 2023,OBIA has housed data for a total of 937 individuals,4136 studies,24,701 series,and 1,938,309 images covering 9 modalities and 30 anatomical sites.Collectively,OBIA provides a reliable platform for biomedical imaging data management and offers free open access to all publicly available data to support research activities throughout the world.OBIA can be accessed at https://ngdc.cncb.ac.cn/obia.
