Tissue Culture and Rapid Propagation of Pedicels of Hemerocallis hybrida

[ Objective] This study aimed to develop tissue culture and rapid propagation methods for pedicels of HemerocaUis hybrida. [ Method] Tender pedicels of dwarf H. hybrida were used as experimental materials to sdect callus induction, subculture and rooting media for rapid propagation of H. hybrida.[ Result ] MS ＋ 2.0 - 3.0 mg/L 6-BA ＋ 0.2 - 0.3 mg/L NAA, MS ＋ 1.0 - 2.0 mg/L 6-BA ＋ 0.1 - 0.2 mg/L NAA, MS and 1/2MS ＋ 0.2 mg/L NAA were the appropriate me- dium for callus induction, subculture and rooting, respectively. [ Conclusion] The in vitro culture and clustered seedling rooting technology used in this study are effective methods for rapid propagation of H. hybrida, which provide technieal reference for industrialized production of H. hybrida.

加味麻黄温经汤联合针灸治疗颈源性头晕临床疗效分析

目的观察加味麻黄温经汤联合针灸治疗颈源性头晕的临床疗效。方法将纳入研究的102例颈源性头晕患者,按自愿接受治疗方法分成两组。对照组48例,给予盐酸氟桂利嗪胶囊联合氯唑沙宗片治疗;观察组54例,给予加味麻黄温经汤联合针灸治疗。两组治疗结束后对比治疗效果。结果治疗后两组头晕症状、NPQ及ESCV评分均降低,且观察组各项评分优于对照组(P<0.05)。观察组总有效率94.44%,显著高于对照组79.17%,组间差异有统计学意义(P<0.05)。结论加味麻黄温经汤联合针灸治疗颈源性头晕效果显著,值得临床应用和推广。

Effects of increased human tumor necrosis factor-like molecule 1A expression in peripheral blood of children with acute Guillain-Barre syndrome on interferon-gamma secretion

BACKGROUND： Human tumor necrosis factor-like molecule 1A （hTL1A） is a strong T helper cell type 1 （Thl） co-stimulator. Guillain-Barre syndrome （GBS） is an autoimmune disorder of the nervous system, which is mediated by Thl cells. OBJECTIVE： To determine hTL1A expression in peripheral blood T lymphocytes of acute GBS children and the effects of hTL1A on secretion of interferon-γ. DESIGN, TIME AND SETTING： A randomized, controlled, neuroimmunological in vitro study was performed at the Central Laboratory of First Hospital of Jilin University, China from November 2005 to November 2007. MATERIALS： Venous blood samples were obtained from 6 healthy donors, aged 6-12 years （all routine blood examination items were normal）, and 6 additional children with acute GBS, aged 6-12 years. The GBS children fell ill within 1 week and were not treated with hormones or immunoglobulin Purified recombinant human soluble tumor necrosis factor-like molecule 1A （rhsTL1A, 1 mg/mL, relative molecular mass 22 000, 6× His tag, soluble form） was supplied by the Central Laboratory of First Hospital of Jilin University, China. METHODS： Peripheral blood mononuclear cells were isolated from healthy donors using the standard Ficoll gradient centrifugation and were incubated in 96-well culture plates. The cells were assigned to the following groups： control （2 μg/mL phytohemagglutinin）, 2μg/mL phytohemagglutinin ＋ 25, 100 and 400 ng/mL rhsTL1A. T cell proliferation was quantified using the tritiated thymidine （3H-TdR） method. Serum interferon-γ levels in acute GBS children were detected by enzyme-linked immunosorbent assay （ELISA）. The ratio of hTL1A-positive T cells to CD3-positive T cells in peripheral blood of acute GBS children was determined using flow cytometry. Following in vitro pre-activation of peripheral blood mononuclear cells by 2 μg/mL phytohemagglutinin, the peripheral blood mononuclear cells were treated with 400 ng/mL exogenous rhsTLIA. Finally, peripheral blood mononuclear cell-secreted interferon-γlevels were measured by ELISA. MAIN OUTCOME MEASURES： The following parameters were measured： rhsTLIA stimulation index to stimulate proliferation of T cells; the serum interferon-γ levels in acute GBS children; the ratio of hTL1A-positive cells to CD3-positive cells; the levels of interferon-γ secreted by peripheral blood mononuclear cells in acute GBS children, as well as rhsTL1A-stimulated interferon-γ levels. RESULTS： T cell proliferation assay revealed that the stimulation index in each rhsTL1A group was greater than the control group. The stimulation index of the 400 ng/mL rhsTL1A group was the greatest. Serum interferon-γ levels in acute GBS children were significantly greater than the control group （P 〈 0.05）. The ratio of hTLIA＋ CD3＋ T cells to CD3＋ T cells in acute GBS children was significantly greater than the control group （P 〈 0.01 ）. Phytohemagglutinin stimulated peripheral blood mononuclear cells to a greater extent than 400 ng/mL rhsTL1A in the acute GBS group, and the secreted interferon-γ levels were significantly increased （P 〈 0.05）. CONCLUSION： In T cells pre-activated with 2 μg/mL phytohemagglutinin, proliferation was effectively increased with 400 ng/mL rhsTL1A treatment. Expression of hTLIA was increased in activated T cells from peripheral blood of acute GBS children, followed by increased interferon-γ secretion. These mechanisms are considered to be part of the pathological process that induces the secretion of inflammatory cytokines in GBS syndrome.

Comparative Experiment of Ground Cucumber Varieties for Open Field Cultivation

Seven ground cucumber varieties were cultivated in open field for the comparative experiment. The results showed that Texuan cucumber and Yantai ground cucumber had good commodity characters,good taste and high yield,and their output value exceeded 16 000 yuan/666. 7 m2,so they are more suitable for open cultivation in Yantai region. The next ones are Meiyu Diguawang and Aweishi ground cucumber.

Thermal-annealing-regulated plasmonic enhanced fluorescence platform enables accurate detection of antigen/antibody against infectious diseases

Plasmonic enhanced fluorescence(PEF)technology is a powerful strategy to improve the sensitivity of immunofluorescence microarrays(IFMA),however,current approaches to constructing PEF platforms are either expensive/time-consuming or reliant on specialized instruments.Here,we develop a completely alternative approach relying on a two-step protocol that includes the self-assembly of gold nanoparticles(GNPs)at the water–oil interface and subsequent annealing-assisted regulation of gold nanogap.Our optimized thermal-annealing GNPs(TA-GNP)platform generates adequate hot spots,and thus produces high-density electromagnetic coupling,eventually enabling 240-fold fluorescence enhancement of probed dyes in the near-infrared region.For clinical detection of human samples,TA-GNP provides super-high sensitivity and low detection limits for both hepatitis B surface antigen and SARS-CoV-2 binding antibody,coupled with a much-improved detection dynamic range up to six orders of magnitude.With fast detection,high sensitivity,and low detection limit,TA-GNP could not only substantially improve the outcomes of IFMA-based precision medicine but also find applications in fields of proteomic research and clinical pathology.

Novel variants of SYNGAP1 associated epileptic encephalopathy: two cases report and literature review

Background SYNGAP1 is a signifcant genetic risk factor for global developmental delay,autism spectrum disorder,and epileptic encephalopathy.De novo loss-of-function variants in this gene cause a neurodevelopmental disorder,for example,early-onset and drug-refractory seizures.We report two children with global developmental delay and epileptic encephalopathy,which are caused by SYNGAP1 gene novel mutations,and drug treatment is efective.Case presentation We report a boy and a girl presented with global developmental delay when they were young babies;as they grew up,cognitive impairment and social-communication disorder became more and more prominent;unfortunately,the patients developed into various seizure types,including eyelid myoclonia,myoclonic and absences when the boy was 1 year 8 mouths old and the girl was 3 years old.The two patients were found two previously unknown mutations by high throughput sequencing[c.3271_c.3272insT;(p.L1091L fs^(*)62),c.2515A>T(p.K839^(*))]in exon 15 of the SYNGAP in the proband.Sanger sequencing confrmed the heterozygous nature,and neither of their parents carried the same mutation.The girl treated with valproic acid and prednisone became seizure-free,and valproic acid and levetiracetam combined with clonazepam were infuential in the other.Conclusions The global developmental delay and epileptic encephalopathy of the children were probably due to the pathogenic mutation of the SYNGAP1 gene,and prednisone and clonazepam may be efective in achieving seizure-free.