Atherosis-associated lnc_000048 activates PKR to enhance STAT1-mediated polarization of THP-1 macrophages to M1 phenotype

Our previous study has demonstrated that lnc_000048 is upregulated in large-artery atherosclerotic stroke and promotes atherosclerosis in ApoE^(-/-)mice.However,little is known about the role of lnc_000048 in classically activated macrophage(M1)polarization.In this study,we established THP-1-derived testing state macrophages(M0),M1 macrophages,and alternately activated macrophages(M2).Real-time fluorescence quantitative PCR was used to verify the expression of marker genes and the expression of lnc_000048 in macrophages.Flow cytometry was used to detect phenotypic proteins(CD11b,CD38,CD80).We generated cell lines with lentivirus-mediated upregulation or downregulation of lnc_000048.Flow cytometry,western blot,and real-time fluorescence quantitative PCR results showed that down-regulation of lnc_000048 reduced M1 macrophage polarization and the inflammation response,while over-expression of lnc_000048 led to the opposite effect.Western blot results indicated that lnc_000048 enhanced the activation of the STAT1 pathway and mediated the M1 macrophage polarization.Moreover,catRAPID prediction,RNA-pull down,and mass spectrometry were used to identify and screen the protein kinase RNA-activated(PKR),then catRAPID and RPIseq were used to predict the binding ability of lnc_000048 to PKR.Immunofluorescence(IF)-RNA fluorescence in situ hybridization(FISH)double labeling was performed to verify the subcellular colocalization of lnc_000048 and PKR in the cytoplasm of M1 macrophage.We speculate that lnc_000048 may form stem-loop structure-specific binding and activate PKR by inducing its phosphorylation,leading to activation of STAT1 phosphorylation and thereby enhancing STAT1 pathway-mediated polarization of THP-1 macrophages to M1 and inflammatory factor expression.Taken together,these results reveal that the lnc_000048/PKR/STAT1 axis plays a crucial role in the polarization of M1 macrophages and may be a novel therapeutic target for atherosclerosis alleviation in stroke.

Early development,life history and ecological habits of Grateloupia constricata Li et Ding

As the largest genus of Halymeniaceae,Grateloupia has been widely reported.Here,we observed the life history and early development of Grateloupia constricata Li et Ding and investigated the effects of temperature,irradiance,and photoperiod on the discoid crust and sporeling development of G.constricata under laboratory conditions.We observed that the type of carpospore development was“mediate discal type”.The life history included homotypic gametophyte(haploid),carposporophyte(diploid),and tetrasporophyte(diploid),with typical isomorphic alternation of generations.The results of double factorial analysis showed that both single factorial effects and interaction among temperature,photoperiod,and irradiance were obviously significant on the discoid crust and sporeling development.Furthermore,we found that the optimum combination of condition for the early growth and development of G.constricata was temperature 20°C,irradiance 80μmol photons/(m^2·s)and photoperiod 16L:8D.This study provides the theoretical basis and technical support for the conservation of the Grateloupia germplasm,artificial breeding,large-scale cultivation and sustainable development.

On the stress–strain states of cellular materials under high loading rates

A virtual Taylor impact of cellular materials is analyzed with a wave propagation technique, i.e. the Lagrangian analysis method, of which the main advantage is that no pre-assumed constitutive relationship is required. Time histories of particle velocity, local strain, and stress profiles are calculated to present the local stress-strain history curves, from which the dynamic stress-strain states are obtained. The present results reveal that the dynamic-rigid-plastic hardening （D-R-PH） material model introduced in a previous study of our group is in good agreement with the dynamic stress-strain states under high loading rates obtained by the Lagrangian analysis method. It directly reflects the effectiveness and feasibility of the D-R-PH material model for the cellular materials under high loading rates.

Molecular detection of SARS-CoV-2 being challenged by virus variation and asymptomatic infection

Coronavirus disease 2019(COVID-19)has been a pandemic for more than a year.With the expanding second wave of the pandemic in winter,the continuous evolution of SARS-CoV-2 has brought new issues,including the significance of virus mutations in infection and the detection of asymptomatic infection.In this review,we first introduced several major SARS-CoV-2 mutations since the COVID-19 outbreak and then mentioned the widely used molecular detection techniques to diagnose COVID-19,primarily focusing on their strengths and limitations.We further discussed the effects of viral genetic variation and asymptomatic infection on the molecular detection of SARS-CoV-2 infection.The review finally summarized useful insights into the molecular diagnosis of COVID-19 under the special situation being challenged by virus mutation and asymptomatic infection.

Dynamic crushingof cellular materials: a particle velocity-based analytical method and its application

Cellular material under high-velocity impacthas a typical feature oflayer-wise collapse.A cell-based finite element model is employed to simulate the direct impact of closed-cell foam, and one-dimensional velocity field distributionsareobtained to characterize thecrushing bandpropagating through a cellular material. An explicit expression of continuous velocity distribution is derivedbased on the features of velocity gradient distribution. The velocity distribution function is adopted to determine the dynamic stress-strain statesof cellular materials under dynamic loading.The local stress-strain history distribution reveals that sectional cells experience a process from the precursor of elastic behavior to the shock stress state, through the dynamic initial crushing state. A power-law relation between the dynamic initial crushing stress andthe strainrate isestablished, which confirms the strain-rate effect of cellular materials. By extracting the critical points immediately before the unloading stage on the local dynamic stress-strain history curves, the dynamic stress-strain statesof cellular materials are determined. They exhibit loading rate-dependence but are independent of the initial impact velocity.Furthermore, with the increase of relative density, the dynamic hardening behaviorof cellular specimen is enhanced and the crushing process event is advanced. The particle velocity-based analytical method is appliedto analyze the dynamic responses of cellular materials.This method is better than continuum-based shock models, since itdoes not require a pre-assumed constitutive relation.Therefore,the particle velocity-based analytical method proposed in this study may provide new ideas to carry out dynamic experimental measurement, which is especially applicable toinhomogeneous materials.

Functional monodisperse microspheres fabricated by solvothermal precipitation co-polymerization

Simultaneous achievement in high solid content and high microsphere yield is deemed a challenge in the fabrication of monodisperse microspheres by precipitation polymerization.We herein demonstrate that micro-sized monodisperse poly(methacrylic monomer-divinylbenzene)microspheres containing epoxy,lauyl,carboxyl and hydroxyl functions can be fabricated by solvothermal precipitation copolymerization at 20%(mass)monomer loading with over 94%microsphere yield.The morphology and porosity of the obtained particles can be readily tuned by cosolvent-acetonitrile binary solvents.Addition of a small amount of cosolvent that has similar solubility parameter to that of the functional monomer can significantly improve the monodispersity of the obtained microspheres.When tetrahydrofuran was used as the co-solvent,the surface area of the highly porous microspheres achieved higher than 400 m^(2)·g^(-1).Solvothermal precipitation co-polymerization can be expected in scale-up fabrication of various monodisperse functional microspheres free of any surfactant and additive.

Dynamic modeling of ultra-precision fly cutting machine tool and the effect of ambient vibration on its tool tip response

The dynamic performances of an ultra-precision fly cutting machine tool(UFCMT)has a dramatic impact on the quality of ultra-precision machining.In this study,the dynamic model of an UFCMT was established based on the transfer matrix method for multibody systems.In particular,the large-span scale flow field mesh model was created;and the variation in linear and angular stiffness of journal and thrust bearings with respect to film thickness was investigated by adopting the dynamic mesh technique.The dynamic model was proven to be valid by comparing the dynamic characteristics of the machine tool obtained by numerical simulation with the experimental results.In addition,the power spectrum density estimation method was adopted to simulate the statistical ambient vibration excitation by processing the ambient vibration signal measured over a long period of time.Applying it to the dynamic model,the dynamic response of the tool tip under ambient vibration was investigated.The results elucidated that the tool tip response was significantly affected by ambient vibration,and the isolation foundation had a good effect on vibration isolation.

Nomenclature Origin and Development of Bi Syndrome Prescriptions

More than 40 medical history and classical medical literature on the nomenclature of Bi syndrome were systematically arranged,induced and analyzed,the prescriptions named after the medicine include RHIZOMA ET RADIX NOTOPTERYGII,os tigris,RADIX ASTRAGALI,RADIX SAPOSHNIKOVIAE,Prepared common monkshood daughter root,doubleteeth pubescent angelica root,RADIX ANGELICAE SINENSIS,frankincense,RADIX STEPHANIAE TETRANDRAE,RADIX ACHYRANTHIS BIDENTATAE,myrrh,RADIX ACONITI、RADIX DIPSACI,RHIZOMA GASTRODIAE,ginseng root,HERBA DENDROBII,CORNU SAIGAE TATARICAE,RADIX MORINDAE OFFICINALIS,PORIA,SEMEN COICIS,poison yam,and so on.It basically reflected the ancient and modern treatment of Bi syndrome in traditional Chinese medicine named after the formula.

Research Progress in TCM Treatment of Rheumatoid Arthritis

Rheumatoid arthritis(RA)belongs to the category of"bi syndrome(arthralgia syndrome)"in traditional Chinese medicine(TCM).According to the characteristics of clinical symptoms,there are descriptions of"bone bi(bone arthralgia)","arthroncus of knee"and"drumstick wind"in TCM.Modern physicians have studied the etiology,pathogenesis and treatment of RA from the treatment of the viscera,including the treatment from the spleen,liver and kidney,treatment from ying(nutrient)and wei(defense),and treatment from ethnic medicine by drawing on the theories of ancient physicians and clinical experience.

Tool-tip vibration prediction based on a novel convolutional enhanced transformer

Superior surface finish remains a fundamental criterion in precision machining operations,and tool-tip vibration is an important factor that significantly influences the quality of the machined surface.Physics-based models heavily rely on assumptions for model simplification when applied to complex high-end systems.However,these assumptions may come at the cost of compromising the model's accuracy.In contrast,data-driven techniques have emerged as an attractive alternative for tasks such as prediction and complex system analysis.To exploit the advantages of data-driven models,this study introduces a novel convolutional enhanced transformer model for tool-tip vibration prediction,referred to as CeT-TV.The effectiveness of this model is demonstrated through its successful application in ultra-precision fly-cutting(UPFC)operations.Two distinct variants of the model,namely,guided and nonguided CeT-TV,were developed and rigorously tested on a data set custom-tailored for UPFC applications.The results reveal that the guided CeT-TV model exhibits outstanding performance,characterized by the lowest mean absolute error and root mean square error values.Additionally,the model demonstrates excellent agreement between the predicted values and the actual measurements,thus underlining its efficiency and potential for predicting the tool-tip vibration in the context of UPFC.

多孔材料吸能行为对相对密度和冲击速度的依赖性

多孔材料是一种优异的吸能缓冲材料,但由于其变形模式的非单一性以及动态应力应变曲线的难获取性,其吸能行为对相对密度和冲击速度的依赖性关系还并不完全明朗.论文基于不需要提前作本构假定的波传播法,开展了多孔材料的吸能行为研究.采用多孔材料的细观有限元模型进行Taylor冲击虚拟实验,获取全场质点速度时程曲线,结合Lagrange分析法得到多孔材料的局部应力应变信息,进而探讨了动态吸能性能对材料相对密度和冲击速度的依赖性.研究结果表明多孔材料的吸能行为可依据变形模式分为三个阶段.在冲击模式下,多孔材料单位体积吸能与相对密度成线性增加关系,此时惯性起主导作用;在过渡模式下,惯性的主导作用减弱,单位体积吸能量的增加速率随相对密度的增加而减弱;在准静态模式下,多孔材料只能发生微小的变形,其吸能很少.论文进一步获得了区别于多孔材料准静态应力-应变曲线的动态应力-应变状态曲线,并考察了其与相对密度之间的关系.结果表明:随着相对密度的增加,多孔材料的动态压实应变将变小,而动态塑性平台应力将提高.

DEVELOPMENT OF EXPERIMENTAL METHODS FOR IMPACT TESTING BY COMBINING HOPKINSON PRESSURE BAR WITH OTHER TECHNIQUES

The split Hopkinson pressure bar（SHPB） technique and the wave propagation inverse analysis（WPIA） technique are both extensively used to experimentally investigate the impact behavior of materials, although neither of them alone provides a fully satisfactory analysis. In the present paper, attention is given to new experimental techniques by incorporating a damagemodified constitutive model into the SHPB technique and combining the Hopkinson pressure bar（HPB） technique with WPIA. First, to distinguish the response due to dynamic constitutive behavior and the response due to dynamic damage evolution, the SHPB method incorporating a damage-modified constitutive model is developed, including an explicit damage-modified Zhu–Wang–Tang model and an implicit damage-modified constitutive model. Second, when the SHPB results become invalid, a method of combining new Lagrange inverse analyses with the HPB technique is developed, including cases of the HPB arranged in front of a long specimen and behind the specimen. As examples of these new methods, typical results are given for nonlinear viscoelastic polymers and concretes considering damage evolution, a super-elastic Ti–Ni alloy with phase transformation and an aluminum foam with shock waves propagating within it.

Hybrid multibody system method for the dynamic analysis of an ultra‐precision fly‐cutting machine tool

The dynamics of an ultra‐precision machine tool determines the precision of the machined surface.This study aims to propose an effective method to model and analyze the dynamics of an ultra‐precision fly‐cutting machine tool.First,the dynamic model of the machine tool considering the deformations of the cutter head and the lathe head is developed.Then,the mechanical elements are classified into M subsystems and F subsystems according to their properties and connections.The M‐subsystem equations are formulated using the transfer matrix method for multibody systems(MSTMM),and the F‐subsystem equations are analyzed using the finite element method and the Craig-Bampton reduction method.Furthermore,all the subsystems are assembled by combining the restriction equations at connection points among the subsystems to obtain the overall transfer equation of the machine tool system.Finally,the vibration characteristics of the machine tool are evaluated numerically and are validated experimentally.The proposed modeling and analysis method preserves the advantages of the MSTMM,such as high computational efficiency,low computational load,systematic reduction of the overall transfer equation,and generalization of its computational capability to general flexible‐body elements.In addition,this study provides theoretical insights and guidance for the design of ultra‐precision machine tools.

Dynamic Testing Technique Based on Multi-channel Photonic Doppler Velocimetry for Investigating the Dynamic Behavior of Materials

In the present paper,more attention is paid to develop a new optical measurement approach of split-Hopkinson pressure bar(SHPB)and wave propagation inverse analysis(WPIA)by using multi-channel photonic Doppler velocimetry(PDV).Based on the particle velocities measured by PDV,the dynamic stress-strain curve of material is obtained in SHPB tests.The strain is determined by the radial particle velocity of specimen,and the stress is determined by the free surface particle velocity of the transmit ted bar.The results obtained by the new method coincide with those obtained by the conventional strain gauge measurements.The new method is non-intrusive and insensitive to electrical noise,making it significantly more reliable than strain gauges.Using the oblique incidence of laser beam,a series of particle velocity wave propagation signals for long rod specimen are measured simultaneously.Based on the measurements of particle velocity profile,the dynamic constitutive response of polymethyl methacrylate(PMMA)is det ermined by WPIA method.The comparison bet ween the dynamic st ress-s train curve and the quasi-static one indicates that the strain-rate effect must be taken into account for PMMA.