An electrochemical immunosensor was developed for ultrasensitive detection of microcystin-LR in water. MIL-101, a porous metal-organic frameworks(MOFs) material based on trivalent chromium skeleton were synthesized by...An electrochemical immunosensor was developed for ultrasensitive detection of microcystin-LR in water. MIL-101, a porous metal-organic frameworks(MOFs) material based on trivalent chromium skeleton were synthesized by hydrothermal synthesis method, and loaded with Au nanoparticles(Au NPs) to prepare Au NPs@MIL-101 composite materials which were used as a marker to label anti microcystin-LR(Anti-MC-LR). The composite materials have strong catalytic properties to the oxidation of ascorbic acid. Anti-MC-LR was immobilized on glassy carbon electrode surface using electrodeposition graphene oxide(GO) as an immobilization matrix to construct a competitive microcystin-LR immunosensor. The electrochemical immunosensor display linear relationship in the range of 0.05 ng/mL-75 μg/mL with linear correlation coefficient of 0.9951 and detection limit of 0.02 ng/mL(S/N = 3). This sensor was used to detect microcystin-LR in the water sample. The recovery was 102.43%,which is satisfied. The good testing results indicate the sensor has a great prospect in practical application.展开更多
基金supported by the National Natural Science Foundation of China(Nos. 21165023,21465026, 21765026, 21605130)the National Key Scientific Program of China(Nos. 2011CB911000,01100205020503104)
文摘An electrochemical immunosensor was developed for ultrasensitive detection of microcystin-LR in water. MIL-101, a porous metal-organic frameworks(MOFs) material based on trivalent chromium skeleton were synthesized by hydrothermal synthesis method, and loaded with Au nanoparticles(Au NPs) to prepare Au NPs@MIL-101 composite materials which were used as a marker to label anti microcystin-LR(Anti-MC-LR). The composite materials have strong catalytic properties to the oxidation of ascorbic acid. Anti-MC-LR was immobilized on glassy carbon electrode surface using electrodeposition graphene oxide(GO) as an immobilization matrix to construct a competitive microcystin-LR immunosensor. The electrochemical immunosensor display linear relationship in the range of 0.05 ng/mL-75 μg/mL with linear correlation coefficient of 0.9951 and detection limit of 0.02 ng/mL(S/N = 3). This sensor was used to detect microcystin-LR in the water sample. The recovery was 102.43%,which is satisfied. The good testing results indicate the sensor has a great prospect in practical application.
