In this study, chemical reduced graphene-silver nanoparticles hybrid （AgNPs@CR-GO） with close-packed AgNPs structure was used as a conductive matrix to adsorb enzyme and facilitate the electron transfer between im- ...In this study, chemical reduced graphene-silver nanoparticles hybrid （AgNPs@CR-GO） with close-packed AgNPs structure was used as a conductive matrix to adsorb enzyme and facilitate the electron transfer between im- mobilized enzyme and electrode. A facile route to prepare AgNPs@CR-GO was designed involving in β-cyclodextrin （β-CD） as reducing and stabilizing agent. The morphologies of AgNPs were regulated and controlled by various experimental factors. To fabricate the bioelectrode, AgNPs@CR-GO was modified on glassy carbon electrode followed by immobilization of glucose oxidase （GOx） or laccase. It was demonstrated by electrochemical testing that the electrode with close-packed AgNPs provided high GOx loading （Г=4.80 × 10^- l0 mol·cm^-2） and fast electron transfer rate （ks=5.76 s^-1）. By employing GOx based-electrode as anode and laccase based-electrode as cathode, the assembled enzymatic biofuel cell exhibited a maximum power density of 77.437 μW·cm^-2 and an open-circuit voltage of 0.705 V.展开更多
基金This research is supported by the National Natural Science Foundation of China （Grant 51372206）.
文摘In this study, chemical reduced graphene-silver nanoparticles hybrid （AgNPs@CR-GO） with close-packed AgNPs structure was used as a conductive matrix to adsorb enzyme and facilitate the electron transfer between im- mobilized enzyme and electrode. A facile route to prepare AgNPs@CR-GO was designed involving in β-cyclodextrin （β-CD） as reducing and stabilizing agent. The morphologies of AgNPs were regulated and controlled by various experimental factors. To fabricate the bioelectrode, AgNPs@CR-GO was modified on glassy carbon electrode followed by immobilization of glucose oxidase （GOx） or laccase. It was demonstrated by electrochemical testing that the electrode with close-packed AgNPs provided high GOx loading （Г=4.80 × 10^- l0 mol·cm^-2） and fast electron transfer rate （ks=5.76 s^-1）. By employing GOx based-electrode as anode and laccase based-electrode as cathode, the assembled enzymatic biofuel cell exhibited a maximum power density of 77.437 μW·cm^-2 and an open-circuit voltage of 0.705 V.
