Dear Editor,Embryonic stem cells(ESCs)have been assumed to possess immature mitochondria and to favor anaerobic glycolysis over oxidative phosphorylation(OXPHOS)for energy production.This proposition is largely based ...Dear Editor,Embryonic stem cells(ESCs)have been assumed to possess immature mitochondria and to favor anaerobic glycolysis over oxidative phosphorylation(OXPHOS)for energy production.This proposition is largely based on the findings that ESCs possess globular mitochondria with blurred cristae,and the facts that ESCs have higher glycolysis activity and lower mitochondrial respiration capacity than somatic cells(Kondoh et al.,2007;Folmes et al.,2011,2012;Zhang et al.,2012;Xu et al.,2013;Ito and Suda,2014;Gu et al.,2016).However,recent studies have shown that mitochondrial autophagy and mitochondrial dynamics are pivotal for ESC self-renewal and pluripotency(Todd et al.,2010;Liu et al.,2016,2020;Wang et al.,2019;Zhong et al.,2019).These studies have raised a fundamental question:what is the contribution and functional mechanism of mitochondrial respiration in pluripotency regulation?展开更多
基金We thank Dr Masaru Okabe at Osaka University Japan for providing the B6D2-Tg(CAG/Su9-DsRed2,Acr3-EGFP)RBGS002Osb mice(mito-red)the National Institute for Basic Biology Japan for providing the B6.Cg-Tg(CAG-GFP/LC3)53Nmz/NmzRbrc mice.+3 种基金the National Key R&D Program of China 2018YFA0108402the Strategic Priority Research Program of the Chinese Academy of Sciences XDA16030302the Strategic Collaborative Research Program of the Ferring Institute of Reproductive Medicine Grant No.33,and the National Natural Science Foundation of China Program(31720103907,31570995,31621004),to T.Z.and(31400831)to J.C.The raw data FASTQ files for the RNA sequencing data are deposited in NCBI GEO,GEO:GSE140712(Fig.1D,1E and S4)and ScienceDB,China National Standard GB/T 7714-2015(Fig.S8A,S8F and S8G)The metabolomic data files are deposited in ScienceDB,China National Standard GB/T 7714-2015.
文摘Dear Editor,Embryonic stem cells(ESCs)have been assumed to possess immature mitochondria and to favor anaerobic glycolysis over oxidative phosphorylation(OXPHOS)for energy production.This proposition is largely based on the findings that ESCs possess globular mitochondria with blurred cristae,and the facts that ESCs have higher glycolysis activity and lower mitochondrial respiration capacity than somatic cells(Kondoh et al.,2007;Folmes et al.,2011,2012;Zhang et al.,2012;Xu et al.,2013;Ito and Suda,2014;Gu et al.,2016).However,recent studies have shown that mitochondrial autophagy and mitochondrial dynamics are pivotal for ESC self-renewal and pluripotency(Todd et al.,2010;Liu et al.,2016,2020;Wang et al.,2019;Zhong et al.,2019).These studies have raised a fundamental question:what is the contribution and functional mechanism of mitochondrial respiration in pluripotency regulation?
