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采砂智能监管系统研究与应用 被引量:1
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作者 鄢煜川 许小华 《水资源研究》 2019年第5期483-490,共8页
随着人工智能图像识别技术的蓬勃兴起,应用领域日益拓宽,将其应用到采砂监管领域,实现对传统监管方式的有力改造是大势所趋。本文根据采砂监管业务的实际需要,研究并设计实现了包含人工智能图像识别技术的视频监控、数据采集、传输存储... 随着人工智能图像识别技术的蓬勃兴起,应用领域日益拓宽,将其应用到采砂监管领域,实现对传统监管方式的有力改造是大势所趋。本文根据采砂监管业务的实际需要,研究并设计实现了包含人工智能图像识别技术的视频监控、数据采集、传输存储、智能分析一体化的采砂智能监管信息系统,并应用到鄱阳湖主要采区,为实时监测采区动态数据、维护采区管理秩序提供了有效的技术支撑。 展开更多
关键词 人工智能 图像识别技术 采砂智能监管
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Expanding the range of CRISPR/Cas9-directed genome editing in soybean 被引量:1
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作者 Reqing He Pengxiang Zhang +4 位作者 yuchuan yan Chen Yu Liyun Jiang Youlin Zhu Dong Wang 《aBIOTECH》 CSCD 2022年第2期89-98,共10页
The CRISPR/Cas9 system has been widely applied for plant genome editing.The commonly used SpCas9 has been shown to rely on the protospacer adjacent motif(PAM)sequences in the canonical form NGG and non-canonical NAG.A... The CRISPR/Cas9 system has been widely applied for plant genome editing.The commonly used SpCas9 has been shown to rely on the protospacer adjacent motif(PAM)sequences in the canonical form NGG and non-canonical NAG.Although these PAM sequences are extensively distributed across plant genomes,a broader scope of PAM sequence is required to expand the range of genome editing.Here we report the adoption of three variant enzymes,xCas9,SpCas9-NG and XNG-Cas9,to produce targeted mutation in soybean.Sequencing results determined that xCas9 with the NGG and KGA(contains TGA and GGA)PAMs successfully induces genome editing in soybean genome.SpCas9-NG could recognize NGD(contains NGG,NGA and NGT),RGC(contains AGC and GGC),GAA and GAT PAM sites.In addition,XNG-Cas9 was observed to cleave soybean genomic regions with NGG,GAA and AGY(contains AGC and AGT)PAM.Moreover,off-target analyses on soybean editing events induced by SpCas9 and xCas9 indicated that two high-fidelity Cas9 variants including eSpCas9(enhanced specificity SpCas9)and exCas9(enhanced specificity xCas9)could improve the specificity of the GGA PAM sequence without reducing on-target editing ficiency.These findings significantly expand the scope of Cas9-mediated genome editing in soybean. 展开更多
关键词 CRISPR/Cas9 Genome editing Protospacer adjacent motif SOYBEAN
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