This study aims to further enhance the oil recovery of reservoirs in the Zhong-2 Block of the Gudao Oilfield by identifying the most effective microbial-flooding activator systems and applying them in the field.We beg...This study aims to further enhance the oil recovery of reservoirs in the Zhong-2 Block of the Gudao Oilfield by identifying the most effective microbial-flooding activator systems and applying them in the field.We began by analyzing the structure of the reservoirs'endogenous microbial communities to understand the potential impact of microbial flooding.This was followed by determining commonly used activator systems based on their abilities to stimulate oil-displacement functional bacteria.Through laboratory experiments on oil displacement efficiency and sweep characteristics,we determined the optimal activator injection method(injection ratio)and the requisite bacterial concentration for maximal microbial-flooding efficacy.Finally,we selected the optimal activator systems and applied them to field tests.Our findings suggest the target block is highly receptive to microbial-flooding.In terms of performance,the activator systems ranked as No.3>No.4>No.1>No.2.Interestingly,a deep activator system,when compared to the top-performing No.3 system,exhibited a higher bacterial concentration peak and longer peaking duration.Optimal oil displacement effects were observed at a 1:4 vol ratio between the No.3 activator and deep activator systems,with bacterial concentrations of up to 106 cells/mL or above.Field tests with the selected activator systems,following a specific injection protocol,demonstrated a notable increase in oil production and a reduction in water cut.展开更多
DNase-catalyzed hydrolysis of extracellular DNA(eDNA)has been widely employed to eradicate intractable biofilms.Although aggregation-induced emission(AIE)has become the ideal tool for killing planktonic bacteria,AIE l...DNase-catalyzed hydrolysis of extracellular DNA(eDNA)has been widely employed to eradicate intractable biofilms.Although aggregation-induced emission(AIE)has become the ideal tool for killing planktonic bacteria,AIE luminogens(AIEgens)often lack DNase-mimetic activity,so as to suffer from poor anti-biofilm capacity.Here,an“AIEzyme”,a kind of AIE nanomaterial with enzyme-like activity,is designed and synthesized,where the AIEgens are used as the ligands of Zr-based coordination polymer nanoparticles.Not only does AIEzyme have enduring DNasemimetic activity with low activation energy,but also structural rigidity-stabilized fluorescence.Due to the long-acting hydrolysis for eDNA in biofilm,AIEzyme can efficiently disorganize the established biofilms with good penetrability and realize the healing of superbug-infected wounds under only one dose of AIEzyme.This work provides a strategy to endow ordinary AIEgens with DNase-like and antibiofilm activities.Moreover,AIEzymes can be observed by virtue of their own AIE character,facilitating the study on self-positioning and residual amount of AIEzymes in wounds.The concept“AIEzyme”would hopefully stimulate the tremendous expansion for the tool kits and the application of AIEgens and artificial enzymes.展开更多
Crop simulation models provide alternative, less time-consuming, and cost-effective means of deter- mining the sensitivity of crop yield to climate change. In this study, two dynamic mechanistic models, CERES （Crop E...Crop simulation models provide alternative, less time-consuming, and cost-effective means of deter- mining the sensitivity of crop yield to climate change. In this study, two dynamic mechanistic models, CERES （Crop Environment Resource Synthesis） and APSIM （Agricultural Production Systems Simulator）, were used to simulate the yield of wheat （Triticum aestivum L.） under well irrigated （CFG） and rain-fed （YY） conditions in relation to different climate variables in the North China Plain （NCP）. The study tested winter wheat yield sensitivity to different levels of temperature, radiation, precipitation, and atmospheric carbon dioxide （COa） concentration under CFG and YY conditions at Luancheng Agro-ecosystem Experimental Stations in the NCR The results from the CERES and APSIM wheat crop models were largely consistent and suggested that changes in climate variables influenced wheat grain yield in the NCR There was also significant variation in the sensitivity of winter wheat yield to climate variables under different water （CFG and YY） conditions. While a temperature increase of 2℃ was the threshold beyond which temperature negatively influenced wheat yield under CFG, a temperature rise exceeding 1℃ decreased winter wheat grain yield under YY. A decrease in solar radiation decreased wheat grain yield under both CFG and YY conditions. Although the sensitivity of winter wheat yield to precipitation was small under the CFG, yield decreased significantly with decreasing precipitation under the rain- fed YY treatment. The results also suggest that wheat yield under CFG linearly increased by ≈ 3.5% per 60 ppm （parts per million） increase in CO2 concentration from 380 to560ppm, and yield under YY increased linearly by ≈ 7.0% for the same increase in CO2 concentration.展开更多
基金funded by the National Natural Science Foun-dation of China(No.51974343)the China Postdoctoral Science Foundation(No.2021M703588)the Open Fund of Hubei Key Laboratory of Drilling and Production Engineering for Oil and Gas(Yangtze University)(No.YQZC202307).
文摘This study aims to further enhance the oil recovery of reservoirs in the Zhong-2 Block of the Gudao Oilfield by identifying the most effective microbial-flooding activator systems and applying them in the field.We began by analyzing the structure of the reservoirs'endogenous microbial communities to understand the potential impact of microbial flooding.This was followed by determining commonly used activator systems based on their abilities to stimulate oil-displacement functional bacteria.Through laboratory experiments on oil displacement efficiency and sweep characteristics,we determined the optimal activator injection method(injection ratio)and the requisite bacterial concentration for maximal microbial-flooding efficacy.Finally,we selected the optimal activator systems and applied them to field tests.Our findings suggest the target block is highly receptive to microbial-flooding.In terms of performance,the activator systems ranked as No.3>No.4>No.1>No.2.Interestingly,a deep activator system,when compared to the top-performing No.3 system,exhibited a higher bacterial concentration peak and longer peaking duration.Optimal oil displacement effects were observed at a 1:4 vol ratio between the No.3 activator and deep activator systems,with bacterial concentrations of up to 106 cells/mL or above.Field tests with the selected activator systems,following a specific injection protocol,demonstrated a notable increase in oil production and a reduction in water cut.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA28020503 and XDA28020500)the Innovation Group Project Agricultural Hydrology and Groundwater Sustainability by Hebei Natural Science Foundation(D2021503001).
基金Youth Innovation Team Project for Talent Introduction and Cultivation in Universities of Shandong Province,Grant/Award Number:096-1622002Research Foundation for Distinguished Scholars of Qingdao Agricultural University,Grant/Award Number:663-1117015。
文摘DNase-catalyzed hydrolysis of extracellular DNA(eDNA)has been widely employed to eradicate intractable biofilms.Although aggregation-induced emission(AIE)has become the ideal tool for killing planktonic bacteria,AIE luminogens(AIEgens)often lack DNase-mimetic activity,so as to suffer from poor anti-biofilm capacity.Here,an“AIEzyme”,a kind of AIE nanomaterial with enzyme-like activity,is designed and synthesized,where the AIEgens are used as the ligands of Zr-based coordination polymer nanoparticles.Not only does AIEzyme have enduring DNasemimetic activity with low activation energy,but also structural rigidity-stabilized fluorescence.Due to the long-acting hydrolysis for eDNA in biofilm,AIEzyme can efficiently disorganize the established biofilms with good penetrability and realize the healing of superbug-infected wounds under only one dose of AIEzyme.This work provides a strategy to endow ordinary AIEgens with DNase-like and antibiofilm activities.Moreover,AIEzymes can be observed by virtue of their own AIE character,facilitating the study on self-positioning and residual amount of AIEzymes in wounds.The concept“AIEzyme”would hopefully stimulate the tremendous expansion for the tool kits and the application of AIEgens and artificial enzymes.
基金This study was supported by the National Natural Science Foundation of China （Grant No. 41401104）, Natural Science Foundation of Hebei Province, China （D2015302017）, China Postdoctoral Science Foundation funded project （2015M570167）, and also supported by the Planning Subject of the ＂Twelfth five-year-plan＂ in National Science and Technology for the Rural Development in China （2013BAD11B03-2）, and Science and Technology Planning Project of Hebei Academy of Science （15101）. We are grateful to the editors and anonymous reviewers for their insightful inputs at the review phase of this work.
文摘Crop simulation models provide alternative, less time-consuming, and cost-effective means of deter- mining the sensitivity of crop yield to climate change. In this study, two dynamic mechanistic models, CERES （Crop Environment Resource Synthesis） and APSIM （Agricultural Production Systems Simulator）, were used to simulate the yield of wheat （Triticum aestivum L.） under well irrigated （CFG） and rain-fed （YY） conditions in relation to different climate variables in the North China Plain （NCP）. The study tested winter wheat yield sensitivity to different levels of temperature, radiation, precipitation, and atmospheric carbon dioxide （COa） concentration under CFG and YY conditions at Luancheng Agro-ecosystem Experimental Stations in the NCR The results from the CERES and APSIM wheat crop models were largely consistent and suggested that changes in climate variables influenced wheat grain yield in the NCR There was also significant variation in the sensitivity of winter wheat yield to climate variables under different water （CFG and YY） conditions. While a temperature increase of 2℃ was the threshold beyond which temperature negatively influenced wheat yield under CFG, a temperature rise exceeding 1℃ decreased winter wheat grain yield under YY. A decrease in solar radiation decreased wheat grain yield under both CFG and YY conditions. Although the sensitivity of winter wheat yield to precipitation was small under the CFG, yield decreased significantly with decreasing precipitation under the rain- fed YY treatment. The results also suggest that wheat yield under CFG linearly increased by ≈ 3.5% per 60 ppm （parts per million） increase in CO2 concentration from 380 to560ppm, and yield under YY increased linearly by ≈ 7.0% for the same increase in CO2 concentration.
