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A preliminary study on life history of marine red alga Stylonema alsidii(Zandardini)Drew f.ramosum Zheng et Li
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作者 Bingxin HUANG Yao ZHANG +4 位作者 yue chu Youxuan GUO Junxia LIANG Jianan QIN Lanping DING 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第3期915-922,共8页
Stylonema alsidii(Zandardini)Drew f.ramosum Zheng et Li is a common species in the genus Stylonema,but there are limited reports on morphological taxonomy and a lack of studies on life history and the regulation of ea... Stylonema alsidii(Zandardini)Drew f.ramosum Zheng et Li is a common species in the genus Stylonema,but there are limited reports on morphological taxonomy and a lack of studies on life history and the regulation of early development.The early development,discoid formation,and erect thallus growth of Stylonema alsidii f.ramosum were observed under laboratory conditions.In addition,the influence of main environmental factors such as temperature and light intensity on its development was briefly studied,and various stages of its life history were observed.The result shows:(1)there are two reproductive modes:asexual reproduction and sexual reproduction;(2)the morphology of this species varies in different degrees under laboratory culture conditions;(3)sexual reproduction and asexual reproduction can coexist under suitable conditions.Five pathways of life history were inferred from experimental phenomena.Mature sporophytes form mature gametophytes by producing initially isolated or initially attached monospore,and mature gametophytes form mature sporophytes by fertilization of eggs and sperms.The sexual and asexual reproduction processes of Stylonema alsidii f.ramosum were reported for the first time,and this is also the first report on sexual reproduction in the genus Stylonema. 展开更多
关键词 Stylonema alsidii(Zandardini)Drew f.ramosum Zheng et Li life history environmental factor asexual reproduction sexual reproduction
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Two-stage reproduction derived from cells of thallus could directly contribute to seeds for green tidal algal Enteromorpha(Ulva) prolifera/clathrata bloom,with disclosure of their ephemeral trait
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作者 Bingxin Huang Lanping Ding +4 位作者 Yao Zhang Youxuan Guo Junxia Liang Yanqi Xie yue chu 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2023年第9期101-112,共12页
Green tidal algal Enteromorpha species complete their life cycles by the isomorphic alternation of generations.The provenance of green tide caused by them in the western Yellow Sea has been disputed. The cell reproduc... Green tidal algal Enteromorpha species complete their life cycles by the isomorphic alternation of generations.The provenance of green tide caused by them in the western Yellow Sea has been disputed. The cell reproduction derived from adult thallus was observed on E. clathrata collected from Shantou City, Guangdong Province in this study. Subsequently, it further found that E. proliferia collected from Qingdao City, Shandong Province and Qinhuangdao City, Hebei Province, produced reproductive cells by somatic cells of its early infantile thallus or branch. The latter is functionally similar to that the seedlings of red alga Porphyra yezoensis produce the monospores, and could exquisitely explain the ephemeral or opportunistic trait and environmental adaptation ability of Enteromorpha species. Changes in growth conditions may induce the two types of cell reproduction.They contribute to the bloom, and can effectively reveal the seasonally occurring large-scale and on-year and offyear phenomenon. The latter may have played a decisive role in its formation. This paper analyses the legal status of the species name, the type of generation during bloom, ephemeral traits, the role of microscopic propagule, the area of origin, on-year and off-year phenomenon, early warning and prevention and control of the species, and so on. On this basis, further study on the influence of environmental factors on cell reproduction of early infantile thalli or branches will achieve a positive effect for early warning and prevention and control of the green tidal algal bloom. 展开更多
关键词 green tidal algae Yellow Sea opportunistic trait on-year and off-year early infantile alternation of generations prediction on the occurrence process
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Effects of main ecological factors on the growth of marine green alga Caulerpa sertularioides using the response surface methodology
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作者 Bingxin Huang yue chu +2 位作者 Rongjuan Wang Yixiao Wang Lanping Ding 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2023年第11期90-97,共8页
Caulerpa sertularioides is an invasive potential blooming green alga in China but it remains poorly studied.We studied the effects of ecological factors on its growth.Optimum conditions of ecological factors,i.e.,irra... Caulerpa sertularioides is an invasive potential blooming green alga in China but it remains poorly studied.We studied the effects of ecological factors on its growth.Optimum conditions of ecological factors,i.e.,irradiance,temperature,and salinity,for the growth of its fragments were determined in the response surface methodology(RSM).The specific growth rates(SGR)of the fragments were determined in single-factor experiment.The results show that the SGR of C.sertularioides peaked under the conditions of irradiance 37.5μmol/(m~2·s),temperature25℃,and salinity 30.Meanwhile,using the Box-Behnken design,the conditions were further optimized and verified to be:irradiance 39.03μmol/(m~2·s),temperature 25.29℃,and salinity 30.06,under which the SGR reached 4.66%.The results provide new theoretical data and solutions for the cultivation,invasion prediction,and monitoring of Caulerpa species in China and the world.The RSM method may have great potential applications in the environmental adaptation characteristics of new macroalgal cultivars,intensive orientation cultured germplasm,and environmental hazard analysis of cultivated species in the field. 展开更多
关键词 Caulerpa sertularioides FRAGMENT response surface methodology(RSM) ecological factor
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基于ENDVI-SI3特征空间的盐渍化反演模型及风险评估 被引量:2
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作者 张思源 岳楚 +3 位作者 袁国礼 袁帅 庞文强 李俊 《自然资源遥感》 CSCD 北大核心 2022年第4期136-143,共8页
土壤盐渍化是干旱和半干旱地区面临的最严重环境风险,利用特征参量建立特征空间的遥感方法为土壤盐渍化的及时监测与反演提供了更有效、更经济的工具和技术。目前反演盐渍化的特征参量多选用归一化植被指数(normalized difference veget... 土壤盐渍化是干旱和半干旱地区面临的最严重环境风险,利用特征参量建立特征空间的遥感方法为土壤盐渍化的及时监测与反演提供了更有效、更经济的工具和技术。目前反演盐渍化的特征参量多选用归一化植被指数(normalized difference vegetation index,NDVI)和盐分指数(salinity index,SI),缺乏精细化分析与地区适用性。以内蒙古乌拉特前旗为研究区,基于Landsat8 OLI数据,选用引入短波红外波段的增强型归一化植被指数(enhanced normalized difference vegetation index,ENDVI)和半干旱区反演效果最优的盐分指数3(salinity index 3,SI3)构建ENDVI-SI3特征空间,建立改进型盐渍化监测指数(improved salinization monitoring index,ISMI)模型。结果表明,ISMI与土壤含盐量相关系数达0.82,反演精度优于NDVI,EDNVI和SI3(-0.66,-0.70和0.75),在ISMI基础上实现了内蒙古乌拉特前旗土壤盐渍化的定量反演分析与风险评估,为半干旱区盐渍化反演特征空间中特征参量的选取提供了优化思路。 展开更多
关键词 盐渍化 遥感模型 增强型归一化植被指数(ENDVI) 盐分指数3(SI3) 特征空间 风险评估
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甘蓝型油菜BnHY5基因的表达模式及非生物胁迫响应分析 被引量:2
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作者 方艳 岳出 +2 位作者 李洁 郭媛媛 王茂林 《四川大学学报(自然科学版)》 CAS CSCD 北大核心 2020年第2期391-399,共9页
为探究甘蓝型油菜中HY5的表达情况,首先通过甘蓝型油菜基因组数据分析预测HY5及其同源基因HYH(HY5-Homologous),克隆获得了四个HY5(命名为BnHY5)以及三个HYH(命名为BnHYH)重复基因全长cDNA序列.其次,对BnHY5进行了蛋白结构预测、理化性... 为探究甘蓝型油菜中HY5的表达情况,首先通过甘蓝型油菜基因组数据分析预测HY5及其同源基因HYH(HY5-Homologous),克隆获得了四个HY5(命名为BnHY5)以及三个HYH(命名为BnHYH)重复基因全长cDNA序列.其次,对BnHY5进行了蛋白结构预测、理化性质分析,研究结果表明:BnHY5蛋白为不稳定、亲水性蛋白,主要结构域为亮氨酸拉链.最后,探究了甘蓝型油菜中该基因的表达情况,用荧光定量PCR分析了BnHY5重复基因表达模式和响应非生物胁迫的情况,BnHY5四个基因中,BnHY5-2的表达水平最高,BnHY5-1、BnHY5-2不存在组织表达差异且表达水平高于BnHY5-3、BnHY5-4.BnHY5基因不仅响应高温、低温、镉、高盐非生物胁迫,而且参与ABA和GA3激素信号响应,说明BnHY5在逆境胁迫中有重要作用.此外BnHY5重复基因在非生物胁迫下的表达模式发生改变,BnHY5四个基因的表达占比与不同类型胁迫处理相关,存在表达差异. 展开更多
关键词 甘蓝型油菜 BnHY5 非生物胁迫 表达分析 重复基因
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油菜不同根肿病抗性材料的室内鉴定及相关生理分析 被引量:3
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作者 蒋学飞 岳出 +1 位作者 刘双双 王茂林 《四川大学学报(自然科学版)》 CAS CSCD 北大核心 2019年第3期573-580,共8页
结合田间种植的根肿病抗性表现,以菌土法和在蘸根法对多个疑似具有抗性的材料进行室内鉴定,初步筛选出两种对根肿菌4号生理小种具有抗性的油菜材料Kc84-1和OG-13;用病情统计的方法将Kc84-1与目前已推广种植的抗性材料华双5R和易感材料R-... 结合田间种植的根肿病抗性表现,以菌土法和在蘸根法对多个疑似具有抗性的材料进行室内鉴定,初步筛选出两种对根肿菌4号生理小种具有抗性的油菜材料Kc84-1和OG-13;用病情统计的方法将Kc84-1与目前已推广种植的抗性材料华双5R和易感材料R-197进行对照验证,并对三种材料进行抗性生理分析.结果表明:Kc84-1与华双5R具有相同水平的根肿病抗性;不同材料的防御酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)活性以及抗病相关物质丙二醛(MDA)含量的表现各不相同,两种抗性材料在较高感病等级下的防御酶SOD、POD、PAL活性显著高于感病材料,PPO活性和MDA含量在抗性材料和易感材料之间没有显著差异.通过水培法观测三种材料感病早期的根毛侵染水平,结果表明,易感材料R-197的根毛侵染率在侵染第6 d到第14 d显著高于抗性材料华双5R和Kc84-1. 展开更多
关键词 油菜 根肿病 抗性鉴定 抗性生理 根毛侵染
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甘蓝型油菜BnTCP7基因的克隆和表达分析 被引量:2
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作者 刘双双 蒋学飞 +1 位作者 岳出 王茂林 《西北植物学报》 CAS CSCD 北大核心 2018年第7期1187-1193,共7页
该研究克隆获得了甘蓝型油菜预测转录因子TCP7-like同源编码基因,命名为BnTCP7,与甘蓝型油菜TCP7-like NC_027757基因的核苷酸序列相似性为95.68%。BnTCP7基因开放阅读框全长为648bp,编码215个氨基酸。BnTCP7氨基酸序列与十字花科(Brass... 该研究克隆获得了甘蓝型油菜预测转录因子TCP7-like同源编码基因,命名为BnTCP7,与甘蓝型油菜TCP7-like NC_027757基因的核苷酸序列相似性为95.68%。BnTCP7基因开放阅读框全长为648bp,编码215个氨基酸。BnTCP7氨基酸序列与十字花科(Brassicaceae)中其他19条TCP7或者TCP7-like氨基酸序列比对发现,BnTCP7与芸薹属和萝卜属等TCP7同源蛋白具有很强的相似性和保守性,尤其在靠近N端的TCP保守结构域,具有典型的螺旋-环-螺旋结构,且BnTCP7属于TCP家族Ⅰ类,为亲水性不稳定蛋白。通过BnTCP7和拟南芥(Arabidopsis thaliana)AtTCP7(NC_003076)蛋白的二级和三级结构在线预测比对分析,进一步证实BnTCP7具有TCP家族典型结构。进化树分析表明,BnTCP7蛋白与甘蓝型油菜TCP7-like NC_027757蛋白聚在同一分支,两者亲缘关系最近。利用转录组数据对不同时期不同器官BnTCP7基因表达模式分析发现,其表达量呈现一定差异性,其中营养器官中的表达量高于生殖器官。非生物胁迫及激素处理对甘蓝型油菜幼苗植株中BnTCP7基因的表达影响分析发现,BnTCP7基因不仅响应了冷、热、损伤等非生物胁迫,而且参与了ABA和GA3激素的信号转导,表明BnTCP7基因在植物维持正常生长发育和逆境胁迫中可能发挥重要作用。 展开更多
关键词 甘蓝型油菜 TCP7转录因子 非生物胁迫 ABA GA3
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Effect of Ti Particles on the Microstructure and Mechanical Properties of AZ91 Magnesium Matrix Composites
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作者 Lu Xiao Ting-Ting Liu +5 位作者 yue chu Bo Song Jie Zhao Xian-Hua Chen Kai-Hong Zheng Fu-Sheng Pan 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 2024年第3期513-524,共12页
Spherical micro-Ti particle(TiP)-reinforced AZ91 magnesium alloy composites were fabricated by semi-solid stirring assisted ultrasonic vibration,which were then subjected to hot extrusion.The microstructure results sh... Spherical micro-Ti particle(TiP)-reinforced AZ91 magnesium alloy composites were fabricated by semi-solid stirring assisted ultrasonic vibration,which were then subjected to hot extrusion.The microstructure results showed that the addition of Ti particles refined the grain size and decreased the texture intensity of the as-extruded AZ91 alloy.An Al3Ti phase with a thickness of 100 nm formed at the Ti/Mg interface,which had a non-coherent relationship with the magnesium matrix.The as-extruded 1 vol.%TiP/AZ91 composite exhibited the best comprehensive mechanical properties,with yield strength,ultimate tensile strength,and elongation at break of 366 MPa,456 MPa,and 14.6%,respectively,which were significantly higher than those of the AZ91 alloy.Therefore,the addition of Ti particles can improve the strength and ductility of the AZ91 alloy,demonstrating the value of magnesium matrix composites for commercial applications. 展开更多
关键词 Magnesium matrix composites Ti particles INTERFACE Mechanical properties
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