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基于UPLC-MS/MS代谢组学技术分析归芪益母口服液对产后奶牛血浆代谢物的影响 被引量:3
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作者 孔维怡 王菲菲 +3 位作者 岳康 秦俊杰 朱浩 郭延生 《中国畜牧兽医》 CAS CSCD 北大核心 2023年第3期913-923,共11页
【目的】通过超高效液相色谱-质谱法(UPLC-MS/MS)代谢组学技术分析归芪益母口服液对产后奶牛血浆代谢物的影响,以阐明归芪益母口服液对产后奶牛的保健机制。【方法】选取15头体况相近、2~3胎健康待产荷斯坦奶牛,随机分为对照组(n=7)和... 【目的】通过超高效液相色谱-质谱法(UPLC-MS/MS)代谢组学技术分析归芪益母口服液对产后奶牛血浆代谢物的影响,以阐明归芪益母口服液对产后奶牛的保健机制。【方法】选取15头体况相近、2~3胎健康待产荷斯坦奶牛,随机分为对照组(n=7)和试验组(n=8),分别在产后立即灌服饮用水和归芪益母口服液,500 mL/头,连续灌服6 d。产后7 d晨饲前采血,经预处理后采用UPLC-MS/MS上机检测,利用MetaboAnanlyst 5.0软件筛选VIP≥1、P<0.05的差异代谢物进行代谢通路富集分析。【结果】产后7 d,试验组血浆代谢轮廓较对照组发生明显改变,筛选出16种差异代谢物,其中8种显著上调,包括蛋氨酸亚砜、磷酸肌酸、甘油磷酸胆碱、皮质醇、L-色氨酸、溶血磷脂酰胆碱(18∶2)、五羟色胺和L-精氨酸;8种显著下调,包括1-氨基环己酸、3-甲基巴豆酰甘氨酸、L-天冬酰胺、单酰甘油酯酰基(16∶1)、泛酰巯基乙胺、十八碳单烯酰胺、1-甲基鸟嘌呤和3-N-甲基-L-组氨酸。差异代谢物显著富集在甘油磷脂代谢、精氨酸和脯氨酸代谢、色氨酸代谢通路。【结论】归芪益母口服液对产后奶牛具有保健作用,可能与其调节甘油磷脂代谢、皮质醇代谢、色氨酸代谢、精氨酸和脯氨酸代谢从而发挥抗炎、抗氧化、调节食欲及肠道菌群稳态、缓解能量负平衡作用有关。 展开更多
关键词 产后奶牛 归芪益母口服液 代谢组学 血浆代谢物 通路富集
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神经反馈训练中的虚拟现实技术综述 被引量:1
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作者 陆凯 岳康 +3 位作者 胡昊辰 沙浩 刘越 王涌天 《计算机辅助设计与图形学学报》 EI CSCD 北大核心 2023年第8期1150-1161,共12页
随着虚拟现实技术及脑-机接口技术的快速发展,虚拟现实在认知训练及神经康复等领域得到了广泛的应用,逐渐成为相关领域研究的热点.与传统的认知训练方法相比,应用于神经反馈训练中的虚拟现实技术通过将用户真实的训练环境替换为可定制... 随着虚拟现实技术及脑-机接口技术的快速发展,虚拟现实在认知训练及神经康复等领域得到了广泛的应用,逐渐成为相关领域研究的热点.与传统的认知训练方法相比,应用于神经反馈训练中的虚拟现实技术通过将用户真实的训练环境替换为可定制的沉浸式虚拟环境,在显著降低系统使用成本的同时有效地提升了训练绩效.从虚拟现实技术在神经反馈训练领域的优势和应用出发,分析了神经反馈训练机制,讨论了虚拟现实技术的反馈训练方法;结合神经反馈训练中的虚拟现实技术研究现状,提出诱发更明显的脑电特征、设计个性化训练方案、提高反馈信息的传输速率、提高训练过程的舒适度以及融合多模态脑影像技术是未来的发展方向. 展开更多
关键词 虚拟现实 神经反馈 脑-机接口 认知能力 神经康复
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奶牛围产后期血液代谢物动态变化规律研究 被引量:1
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作者 王菲菲 孔维怡 +3 位作者 段静丹 岳康 刘欣 郭延生 《动物营养学报》 CAS CSCD 北大核心 2023年第2期953-967,共15页
本试验旨在揭示奶牛围产后期血液代谢物动态变化规律,为围产后期营养调控和代谢性疾病的预防提供参考依据。选取体况相近、胎次相同的荷斯坦奶牛7头,尾静脉采集产后0(分娩当天)、7、14和21 d血液样本,制备血浆,进行超高液相色谱-串联质... 本试验旨在揭示奶牛围产后期血液代谢物动态变化规律,为围产后期营养调控和代谢性疾病的预防提供参考依据。选取体况相近、胎次相同的荷斯坦奶牛7头,尾静脉采集产后0(分娩当天)、7、14和21 d血液样本,制备血浆,进行超高液相色谱-串联质谱(UPLC-MS/MS)代谢组学和代谢通路分析。结果表明:1)奶牛产后7、14和21 d血液代谢轮廓较产后0 d发生了明显改变,而产后7、14和21 d之间相比,血液代谢轮廓未发生明显变化。2)与产后0 d相比,产后7 d能量代谢相关代谢物丙酮酸、L-乳酸、D-葡萄糖和甜菜碱相对含量显著下调[P<0.05,变量重要性投影(VIP)≥1,差异倍数(FC)≤0.5],脂代谢相关代谢物肉碱C11∶DC、肉碱C12∶1、肉碱C13∶0等和氧化三甲胺(TMAO)相对含量显著下调(P<0.05,VIP≥1,FC≤0.5),氧化应激相关代谢物还原型谷胱甘肽和L-半胱氨酸相对含量显著下调(P<0.05,VIP≥1,FC≤0.5),肾上腺素和皮质醇相对含量显著下调(P<0.05,VIP≥1,FC≤0.5),胆汁酸代谢相关代谢物甘氨胆酸、甘氨脱氧胆酸、23-脱甲脱氧胆酸和甘氨鹅脱氧胆酸含量显著上调(P<0.05,VIP≥1,FC≥2)。研究结果表明,围产后期奶牛处于代谢应激和氧化应激状态,为奶牛产后护理提供了相关理论依据。 展开更多
关键词 奶牛 围产后期 代谢组学 代谢应激 氧化应激
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自拟中药复方制剂对患子宫内膜炎肉母牛外周血淋巴细胞、细胞因子和繁殖激素的影响 被引量:2
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作者 王建东 刘欣 +5 位作者 岳康 郭亚男 侯鹏霞 康晓冬 于洋 梁小军 《动物医学进展》 北大核心 2023年第11期127-132,共6页
自拟配制中药复方制剂,探讨该方对患子宫内膜炎肉母牛外周血淋巴细胞、细胞因子和繁殖激素等的影响。试验选取20头产后患有子宫内膜炎肉母牛,随机分为对照组、中药复方制剂低、中和高3个剂量组。试验组每天分别灌服400 g/头、500 g/头、... 自拟配制中药复方制剂,探讨该方对患子宫内膜炎肉母牛外周血淋巴细胞、细胞因子和繁殖激素等的影响。试验选取20头产后患有子宫内膜炎肉母牛,随机分为对照组、中药复方制剂低、中和高3个剂量组。试验组每天分别灌服400 g/头、500 g/头、600 g/头剂量的中药复方制剂,连续给药14 d。试验结束后尾静脉采集血液样本,预处理后对外周血中T淋巴细胞亚群比例、淋巴细胞转化率和凋亡率、血清中细胞因子、免疫球蛋白和生殖激素的含量进行测定。结果表明,复方中药制剂中、高浓度组与对照组相比,CD4^(+)T细胞比例、IL-4、AMH、E2和INHB均极显著升高(P<0.01),IgM和IgA水平显著升高(P<0.05),淋巴细胞转化率显著提升(P<0.05),细胞凋亡率显著降低(P<0.05);低浓度组与对照组相比CD4^(+)T细胞比例显著升高(P<0.05),IL-6含量极显著降低(P<0.01);在低、中和高浓度组中CD4^(+)/CD8^(+)T细胞较对照组出现极显著升高(P<0.01),CD8^(+)T细胞比例极显著降低(P<0.01)。与低剂量组相比,高浓度组CD8^(+)T细胞比例显著降低(P<0.05),CD4^(+)/CD8^(+)T细胞和AMH极显著升高(P<0.01),CD4^(+)T细胞比例显著提升(P<0.05);中浓度组CD4^(+)/CD8^(+)T细胞显著升高(P<0.05)。说明在中药复方制剂干预后,患子宫内膜炎肉母牛能够显著增强免疫性能,降低炎症反应,改善繁殖激素水平。 展开更多
关键词 中药复方制剂 子宫内膜炎 肉母牛 免疫功能 激素水平
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归芪益母复方制剂对产后奶牛血液氧化脂质的调节作用
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作者 毛永霞 王菲菲 +5 位作者 孔维怡 岳康 王瑞玲 刘欣 丁辉 郭延生 《南方农业学报》 CAS CSCD 北大核心 2023年第12期3697-3707,共11页
【目的】探究归芪益母复方制剂对产后奶牛血液氧化脂质的调节作用,以期进一步明确归芪益母复方制剂抗炎和抗氧化的作用机制,为归芪益母复方制剂在产后奶牛保健与疾病防治中的应用提供参考依据。【方法】选取体况相近、2~3胎次的健康待... 【目的】探究归芪益母复方制剂对产后奶牛血液氧化脂质的调节作用,以期进一步明确归芪益母复方制剂抗炎和抗氧化的作用机制,为归芪益母复方制剂在产后奶牛保健与疾病防治中的应用提供参考依据。【方法】选取体况相近、2~3胎次的健康待产荷斯坦奶牛15头,将其随机分成对照组(n=7)和试验组(n=8),试验组奶牛产后灌服归芪益母复方制剂500 mL/头,连续灌服6 d,对照组奶牛每日灌服等体积饮用水。产后7 d晨饲前于尾静脉采集血液,制备血浆样本,采用超高效液相色谱—串联质谱(UPLC-MS/MS)技术定量检测2组奶牛血浆中氧化脂质含量,分析2组奶牛血浆氧化脂质代谢轮廓的变化规律,筛选产后奶牛血液中的差异氧化脂质并进行代谢通路富集分析。【结果】2组奶牛血浆中共鉴定出44种氧化脂质,其中花生四烯酸及其代谢物18个、亚麻酸及其衍生物12个、二十碳五烯酸及其衍生物9个、二十二碳六烯酸及其衍生物4个、二十二碳五烯酸及其衍生物1个。产后7 d,试验组奶牛血浆中8种氧化脂质代谢轮廓较对照组发生显著变化(P<0.05,下同),其中5种脂质分子显著上调(VIP≥1,FC≥2),包括12-HETE、11-HETE、15-HETE、5-HETE和15-oxoETE;3种脂质分子显著下调(VIP≥1,FC≤0.5),包括20-HETE、8,9-EET和TXB2。代谢通路富集分析结果表明,差异脂质分子主要富集在花生四烯酸代谢通路、TRP通道的炎症介质调节通路和PPAR信号通路。【结论】归芪益母复方制剂主要通过花生四烯酸代谢、TRP通道的炎症介质调节和PPAR信号通路来调节产后奶牛脂质代谢,从而发挥抗炎和抗氧化作用。 展开更多
关键词 奶牛 归芪益母复方制剂 脂质组学 氧化脂质 代谢通路
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银川地区肉牛子宫内膜炎病原菌的分离鉴定与药敏试验
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作者 岳康 郭延生 +2 位作者 刘红燕 郭亚男 王建东 《动物医学进展》 北大核心 2023年第10期119-123,共5页
为掌握银川地区肉牛子宫内膜炎病原菌种类及其常用治疗药物的耐药性情况,采集50份患子宫内膜炎肉牛的子宫分泌物,进行分离培养与16S rRNA鉴定,并对分离所得的病原菌进行药物敏感性分析。结果显示,大肠埃希氏菌检出率为36%,链球菌检出率... 为掌握银川地区肉牛子宫内膜炎病原菌种类及其常用治疗药物的耐药性情况,采集50份患子宫内膜炎肉牛的子宫分泌物,进行分离培养与16S rRNA鉴定,并对分离所得的病原菌进行药物敏感性分析。结果显示,大肠埃希氏菌检出率为36%,链球菌检出率为28%,金黄色葡萄球菌检出率为19%,蜡样芽孢杆菌检出率为10%,产气荚膜梭菌检出率为7%。该16S rRNA序列与NCBI公布的菌株序列同源性大于98%。大肠埃希氏菌对头孢唑啉、米诺环素、头孢他啶高度敏感,链球菌对左氧氟沙星、头孢曲松钠较为敏感,金黄色葡萄球菌对万古霉素完全敏感,蜡样芽孢杆菌对头孢唑啉、链霉素、米诺环素敏感,产气荚膜梭菌对头孢噻肟敏感。由此可见,分离出的病原菌对24种常用抗菌药物存在不同程度的的耐药情况,仅对少数药物敏感。 展开更多
关键词 子宫内膜炎 分离鉴定 药敏试验 耐药性
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基于ECG的立体3D显示视疲劳的评估和建模 被引量:7
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作者 王丹力 杨心盼 +1 位作者 岳康 胡海琛 《系统仿真学报》 CAS CSCD 北大核心 2019年第2期212-217,共6页
通过采集被试在观看3D电影过程中的心电数据,结合主观打分及实验前后问卷、反应时数据来研究观看过程中视疲劳和心电指标的变化规律,找到能够反映视疲劳的心电指标并对视疲劳进行建模。实验分析了12个被试数据,从时域和频域两个方面对... 通过采集被试在观看3D电影过程中的心电数据,结合主观打分及实验前后问卷、反应时数据来研究观看过程中视疲劳和心电指标的变化规律,找到能够反映视疲劳的心电指标并对视疲劳进行建模。实验分析了12个被试数据,从时域和频域两个方面对心电信号进行分析。分析结果发现,主观打分和心率(HR)随着观看时间持续而增大,PNN50随观看时长增加变小。对主观打分进行线性建模,得到模型R?为0.930。此外,主观问卷表明在实验后总体视疲劳,眼睛干涩,感到恶心等症状均发生显著变化(p<0.05)。 展开更多
关键词 立体显示 视疲劳 心率变异性 时频域分析 建模
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New evidence for the links between the local water cycle and the underground wet sand layer of a mega-dune in the Badain Jaran Desert, China 被引量:7
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作者 Jun WEN ZhongBo SU +5 位作者 TangTang ZHANG Hui TIAN YiJian ZENG Rong LIU yue kang Rogier van der VELDE 《Journal of Arid Land》 SCIE CSCD 2014年第4期371-377,共7页
Scientists and the local government have great concerns about the climate change and water resources in the Badain Jaran Desert of western China. A field study for the local water cycle of a lake-desert system was con... Scientists and the local government have great concerns about the climate change and water resources in the Badain Jaran Desert of western China. A field study for the local water cycle of a lake-desert system was conducted near the Noertu Lake in the Badain Jaran Desert from 21 June to 26 August 2008. An underground wet sand layer was observed at a depth of 20–50 cm through analysis of datasets collected during the field experiment. Measurements unveiled that the near surface air humidity increased in the nighttime. The sensible and latent heat fluxes were equivalent at a site about 50 m away from the Noertu Lake during the daytime, with mean values of 134.4 and 105.9 W/m2 respectively. The sensible heat flux was dominant at a site about 500 m away from the Noertu Lake, with a mean of 187.7 W/m2, and a mean latent heat flux of only 26.7 W/m2. There were no apparent differences for the land surface energy budget at the two sites during the night time. The latent heat flux was always negative with a mean value of –12.7 W/m2, and the sensible heat flux was either positive or negative with a mean value of 5.10 W/m2. A portion of the local precipitation was evaporated into the air and the top-layer of sand dried quickly after every rainfall event, while another portion seeped deep and was trapped by the underground wet sand layer, and supplied water for surface psammophyte growth. With an increase of air humidity and the occurrence of negative latent heat flux or water vapor condensation around the Noertu Lake during the nighttime, we postulated that the vapor was transported and condensed at the lakeward sand surface, and provided supplemental underground sand pore water. There were links between the local water cycle, underground wet sand layer, psammophyte growth and landscape evolution of the mega-dunes surrounding the lakes in the Badain Jaran Desert of western China. 展开更多
关键词 mega-dune water cycle observation wet sand layer Badain Jaran Desert
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Numerical study on dynamic mechanism of brain volume and shear deformation under blast loading 被引量:3
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作者 Zhijie Li Zhibo Du +8 位作者 Xiaochuan You Zhanli Liu Jian Cheng Chengcheng Luo Dongyang Chu Shaowu Ning yue kang Ce Yang Zhuo Zhuang 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2019年第5期1104-1119,共16页
Blast-induced traumatic brain injury(b-TBI)is a kind of significant injury to soldiers in the current military conflicts.However,the mechanism of b-TBI has not been well understood,and even there are some contradictor... Blast-induced traumatic brain injury(b-TBI)is a kind of significant injury to soldiers in the current military conflicts.However,the mechanism of b-TBI has not been well understood,and even there are some contradictory conclusions.It is crucial to reveal the dynamic mechanism of brain volume and shear deformations under blast loading for better understanding of b-TBI.In this paper,the numerical simulation method is adopted to carry out comprehensive and in-depth researches on this issue for the first time.Based on the coupled Eulerian-Lagrangian method,the fluid-structure coupling model of the blast wave and human head is developed to simulate two situations,namely the head subjected to the frontal and lateral impacts.The simulation results are analyzed to obtain the underlying dynamic mechanisms of brain deformation.The brain volume deformation is dominated by the local bending vibration of the skull,and the corresponding frequency for the forehead skull under the frontal impact and the lateral skull faced to the lateral impact is as high as 8 kHz and 5 kHz,respectively.This leads to the high-frequency fluctuation of brain pressure and the large pressure gradient along the skull,totally different from the dynamic response of brain under head collisions.While the brain shear deformation mainly depends on the relative tangential displacement between the skull and brain and the anatomical structure of inner skull,being not related to the brain pressure and its gradient.By further comparing the medical statistics,it is inferred that diffuse axonal injury and brain contusion,the two most common types of b-TBI,are mainly attributed to brain shear deformations.And the von Mises stress can be adopted as the indicator for these two brain injuries.This study can provide theoretical guidance for the diagnosis of b-TBI and the development of protective equipment. 展开更多
关键词 Blast-induced traumatic BRAIN INJURY Numerical head MODEL Fluid-structure coupling MODEL Diffuse AXONAL INJURY BRAIN CONTUSION
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Room-Temperature Magnetism of Ceria Nanocubes by Inductively Transferring Electrons to Ce Atoms from Nearby Oxygen Vacancy 被引量:1
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作者 yue kang Qiang Leng +3 位作者 Donglin Guo Dezhi Yang Yanping Pu Chenguo Hu 《Nano-Micro Letters》 SCIE EI CAS 2016年第1期13-19,共7页
Ceria(CeO_2) nanocubes were synthesized by a hydrothermal method and weak ferromagnetism was observed in room temperature. After ultraviolet irradiation, the saturation magnetization was significantly enhanced from*3.... Ceria(CeO_2) nanocubes were synthesized by a hydrothermal method and weak ferromagnetism was observed in room temperature. After ultraviolet irradiation, the saturation magnetization was significantly enhanced from*3.18×10^(-3) to *1.89×10^(-2) emug^(-1). This is due to the increase of oxygen vacancies in CeO_2 structure which was confirmed by X-ray photoelectron spectra. The first-principle calculation with Vienna ab-initio simulation package was used to illustrate the enhanced ferromagnetism mechanism after calculating the density of states(DOSs) and partial density of states(PDOSs) of CeO_2 without and with different oxygen vacancies. It was found that the increase of oxygen vacancies will enlarge the PDOSs of Ce 4f orbital and DOSs. Two electrons in one oxygen vacancy are respectively excited to 4f orbital of two Ce atoms neighboring the vacancy, making these electron spin directions on 4f orbitals of these two Ce atoms parallel. This superexchange interaction leads to the formation of ferromagnetism in CeO_2 at room temperature. Our work indicates that ultraviolet irradiation is an effective method to enhance the magnetism of CeO_2 nanocube, and the firstprinciple calculation can understand well the enhanced magnetism. 展开更多
关键词 UV irradiation Oxygen VACANCIES Saturation MAGNETISM Spin direction SUPEREXCHANGE interaction
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曲霉发酵物对热应激状态奶牛泌乳性能的影响 被引量:1
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作者 钱军 岳康 +2 位作者 方仕瑞 朱军 吴心华 《现代畜牧兽医》 2021年第4期25-27,共3页
为解决宁夏奶牛热应激期间干物质采食量降低导致的产奶量下降问题,试验选择产后90 d的头胎奶牛540头,分为试验组和对照组,饲喂相同质量的TMR日粮。试验组全混合日粮中添加30 g/(d·头)曲霉发酵物。试验周期30 d。结果表明,试验组在... 为解决宁夏奶牛热应激期间干物质采食量降低导致的产奶量下降问题,试验选择产后90 d的头胎奶牛540头,分为试验组和对照组,饲喂相同质量的TMR日粮。试验组全混合日粮中添加30 g/(d·头)曲霉发酵物。试验周期30 d。结果表明,试验组在饲喂曲霉发酵物后第7、14、28 d产奶量显著高于对照组(P<0.05);饲喂曲霉发酵物后第21、28 d干物质采食量显著高于对照组(P<0.05);其他时间试验组和对照组的产奶量和干物质采食量差异不显著(P>0.05)。研究表明,奶牛热应激期间添加30 g/(d·头)曲霉发酵物能够提高干物质采食量和产奶量。 展开更多
关键词 曲霉发酵物 热应激 产奶量
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Iron-based metal-organic framework as a dual cooperative release system for enhanced vascularization and bone regeneration
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作者 Chang Xu yue kang +3 位作者 Shiqiang Guan Xufeng Dong Daqing Jiang Min Qi 《Chinese Chemical Letters》 SCIE CAS CSCD 2023年第5期475-479,共5页
Vascularization and bone regeneration are closely related in the process of bone remodeling,and designing a bioactive scaffold with pro-angiogenic and osteogenic properties may accelerate the repair of bone defects.In... Vascularization and bone regeneration are closely related in the process of bone remodeling,and designing a bioactive scaffold with pro-angiogenic and osteogenic properties may accelerate the repair of bone defects.In this work,an iron-based metal-organic framework(MIL-88)was developed as a carrier for loading a pro-angiogenic small molecular drug(dimethyloxallyl glycine,DMOG),and then embedded into the PLGA nanofibrous scaffolds to repair cranial defects in rats.Imaging and histological evaluation indicated that PLGA/MIL@D scaffold markedly enhanced vascularization and bone regeneration in vivo.Moreover,in vitro assay showed that co-delivery system significantly promoted angiogenesis by stimulating endothelial cell migration,tube formation,and enhanced osteogenesis by promoting expression of osteoblast related proteins.In addition,PLGA/MIL@D scaffold promotes angiogenesis by activating the hypoxia-inducible factor-1(HIF-1)/vascular endothelial growth factor(VEGF)signaling pathway.Altogether,this bioactive PLGA/MIL@D scaffold can combine angiogenesis with osteogenesis,and will be a bright strategy for the repair of bone defects. 展开更多
关键词 OSTEOGENESIS ANGIOGENESIS DMOG Metal-organic framework Bone regeneration
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Integration exosomes with MOF-modified multifunctional scaffold for accelerating vascularized bone regeneration
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作者 Chang Xu yue kang +2 位作者 Xufeng Dong Daqing Jiang Min Qi 《Chinese Chemical Letters》 SCIE CAS CSCD 2023年第2期429-433,共5页
Designing a multifunctional scaffold with osteogenic and angiogenic properties holds promise for ideal bone regeneration. Innovative scaffold was here constructed by immobilizing exosomes derived from human bone mesen... Designing a multifunctional scaffold with osteogenic and angiogenic properties holds promise for ideal bone regeneration. Innovative scaffold was here constructed by immobilizing exosomes derived from human bone mesenchymal stem cells(h BMSCs) onto porous polymer meshes which developed by PLGA and Cu-based MOF(PLGA/Cu BDC@Exo). The synthesized exosome-laden scaffold capable of providing a dual cooperative controllable release of bioactive copper ions and exosomes that promote osteogenesis and angiogenesis, thereby achieving cell-free bone regeneration. In vitro assay revealed the composite stent not only substantially upregulated the expression of osteogenic-related proteins(ALP, Runx2, Ocn)and VEGF in h BMSCs, but promoted the migration and tube formation of the human umbilical vein endothelial cells(HUVECs). In vivo evaluation further confirmed this scaffold dramatically stimulated bone regeneration and angiogenesis in critical-sized defects in rats. Altogether, this composite scaffold carrying therapeutic exosomes had an osteogenic-angiogenic coupling effect and offered a new idea for cell-free bone tissue engineering. 展开更多
关键词 EXOSOMES OSTEOGENESIS ANGIOGENESIS Metal-organic framework Bone regeneration
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Exosome-functionalized magnesium-organic framework-based scaffoldswith osteogenic, angiogenic and anti-inflammatory properties foraccelerated bone regeneration 被引量:14
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作者 yue kang Chang Xu +3 位作者 Ling’ao Meng Xufeng Dong Min Qi Daqing Jiang 《Bioactive Materials》 SCIE 2022年第12期26-41,共16页
Exosomes derived from human adipose-derived stem cells (hADSCs-Exos) have shown potential as an effectivetherapeutic tool for repairing bone defects. Although metal-organic framework (MOF) scaffolds are promisingstrat... Exosomes derived from human adipose-derived stem cells (hADSCs-Exos) have shown potential as an effectivetherapeutic tool for repairing bone defects. Although metal-organic framework (MOF) scaffolds are promisingstrategies for bone tissue regeneration, their potential use for exosome loading remains unexplored. In this study,motivated by the potential advantages of hADSCs-Exos and Mg-GA MOF, we designed and synthesized anexosome-functionalized cell-free PLGA/Mg-GA MOF (PLGA/Exo-Mg-GA MOF) scaffold, taking using of thebenefits of hADSCs-Exos, Mg2+, and gallic acid (GA) to construct unique nanostructural interfaces to enhanceosteogenic, angiogenic and anti-inflammatory capabilities simultaneously. Our in vitro work demonstrated thebeneficial effects of PLGA/Exo-Mg-GA MOF composite scaffolds on the osteogenic effects in human bonemarrow-derived mesenchymal stem cells (hBMSCs) and angiogenic effects in human umbilical endothelial cells(HUVECs). Slowly released hADSCs-Exos from composite scaffolds were phagocytosed by co-cultured cells,stabilized the bone graft environment, ensured blood supply, promoted osteogenic differentiation, and acceleratedbone reconstruction. Furthermore, our in vivo experiments with rat calvarial defect model showed thatPLGA/Exo-Mg-GA MOF scaffolds promoted new bone formation and satisfactory osseointegration. Overall, weprovide valuable new insights for designing exosome-coated nanocomposite scaffolds with enhanced osteogenesisproperty. 展开更多
关键词 Metal-organic framework Exosomes Magnesium ions Gallic acid OSTEOGENESIS Angiogenesis ANTI-INFLAMMATION Rat calvarial defect model Bone regeneration
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Production of aryl oxygen-containing compounds by catalytic pyrolysis of bagasse lignin over LaTi0.2Fe0.8O3 prepared by different methods 被引量:5
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作者 Haiying Wang Hongjing Han +6 位作者 Enhao Sun Yanan Zhang Jinxin Li Yanguang Chen Hua Song Hongzhi Zhao yue kang 《Journal of Rare Earths》 SCIE EI CAS CSCD 2020年第1期76-83,共8页
To realize the resource and high-value utilization,a new approach,named bagasse lignin(BL) used to produce aryl oxygen-containing compounds by catalytic pyrolysis over perovskite,was proposed,LaTi0.2Fe0.8O3(LTF) sampl... To realize the resource and high-value utilization,a new approach,named bagasse lignin(BL) used to produce aryl oxygen-containing compounds by catalytic pyrolysis over perovskite,was proposed,LaTi0.2Fe0.8O3(LTF) samples prepared by the sol-gel method(SG) and the solid-state reaction method(SS)were characterized.The catalytic action on BL pyrolysis was performed by the test of TG-DTG and the evaluation of the fixed bed micro-reactor,the components and contents of the products were determined.The results show that LTF samples have cubic perovskite phase,LTF prepared by SG(LTF-SG) is porous with larger specific surface area than LTF prepared by SS(LTF-SS).During the pyrolysis of BL,the addition of LTF lowers the pyrolysis temperature and the activation energy,the contents of CO2 and CO in gaseous products reduce by 4.6%-8.0% and 30.7%-34.3%,respectively,the total content of aryl oxygencontaining compounds(including phenolics,guaiacols,syringols and phenylates) in liquid products increases from 62 wt% to more than 72 wt%,and LTF-SG shows better catalytic performance.LTF samples have nice phase and catalytic stabilities for BL pyrolysis after five successive redox cycles. 展开更多
关键词 Bagasse lignin Perovskite Catalytic pyrolysis Aryl oxygen-containing compounds Titanium-substituted lanthanum ferrite Rare earths
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Identification of a heat shock cognate protein 70 gene in Chinese soft-shell turtle (Pelodiscus sinensis) and its expression profiles under thermal stress 被引量:3
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作者 Xiao-liang LI yue kang +2 位作者 Xiao-yan ZHANG Bing-lin ZHU Wei-huan FANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2012年第6期465-477,共13页
The heat shock cognate protein 70 (Hsc70) is a member of a 70-kDa heat shock protein (HSP70) family that functions as molecular chaperones.In this study,a novel Hsc70 gene from Chinese soft-shelled turtle (Pelodiscus ... The heat shock cognate protein 70 (Hsc70) is a member of a 70-kDa heat shock protein (HSP70) family that functions as molecular chaperones.In this study,a novel Hsc70 gene from Chinese soft-shelled turtle (Pelodiscus sinensis) (tHsc70) was identified.The tHsc70 full-length complementary DNA (cDNA) is 2 272 bp long with a 1 941-bp open reading frame (ORF) encoding 646 amino acids.Three characteristic signature regions of the HSP70 family,two major domains of an adenosine triphosphate (ATP)/guanosine triphosphate (GTP) binding domain (ABD),and a substrate-binding domain (SBD) were present in the predicted tHsc70 amino acid sequence.The tHsc70 gene was expressed in Escherichia coli BL21 and the expression product reacted with the anti-Hsc70 mouse monoclonal antibody by Western blotting.Homology analysis revealed that tHsc70 shared identity from 53.9% to 87.7% at the nucleotide level,and 49.1% to 99.5% at the amino acid level with the known Hsc70s.Phylogenetic analysis showed that tHsc70 was clustered together with the Hsc70 gene of another reptile species (Alligator mississippiensis).The tHsc70 was expressed in the liver,lung,heart,and skeletal muscle.The expression patterns of tHsc70 messenger RNA (mRNA) differed among different tissues under different durations of heat stress at 40 °C.Adaptation at 25 °C for 1 h after heat stress was also different among tissues and length of heat stress.Irrespective of different profiles of expression under heat stress,tHsc70 may play roles in protecting turtles from thermal stress. 展开更多
关键词 Pelodiscus sinensis 热吃惊血缘的蛋白质 70 (Hsc70 ) 种系发生的分析 热应力
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Natural language processing(NLP)in management research:A literature review 被引量:4
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作者 yue kang Zhao Cai +2 位作者 Chee-Wee Tan Qian Huang Hefu Liu 《Journal of Management Analytics》 EI 2020年第2期139-172,共34页
Natural language processing(NLP)is gaining momentum in management research for its ability to automatically analyze and comprehend human language.Yet,despite its extensive application in management research,there is n... Natural language processing(NLP)is gaining momentum in management research for its ability to automatically analyze and comprehend human language.Yet,despite its extensive application in management research,there is neither a comprehensive review of extant literature on such applications,nor is there a detailed walkthrough on how it can be employed as an analytical technique.To this end,we review articles in the UT Dallas List of 24 Leading Business Journals that employ NLP as their focal analytical technique to elucidate how textual data can be harnessed for advancing management theories across multiple disciplines.We describe the available toolkits and procedural steps for employing NLP as an analytical technique as well as its advantages and disadvantages.In so doing,we highlight the managerial and technological challenges associated with the application of NLP in management research in order to guide future inquires. 展开更多
关键词 natural language processing literature review sentiment analysis machine learning
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Correction to: Numerical study on dynamic mechanism of brain volume and shear deformation under blast loading
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作者 Zhijie Li Zhibo Du +8 位作者 Xiaochuan You Zhanli Liu Jian Cheng Chengcheng Luo Dongyang Chu Shaowu Ning yue kang Ce Yang Zhuo Zhuang 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2020年第4期981-982,共2页
In the original publication few errors have been identified in Fig.10a,b.The correct version of Fig.10a,b are provided in this correction.The original article has been corrected[1].
关键词 corrected LOADING CORRECT
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Cultural Advancement and the Primary Stage of Socialism
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作者 于幼军 Yang Qiushen +1 位作者 yue kang Su Xuetao 《Social Sciences in China》 2000年第3期44-53,共10页
关键词 Cultural Advancement and the Primary Stage of Socialism
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