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基于数据非依赖性采集定量蛋白质组学分析的原发性干燥综合征潜在唾液生物标志物研究
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作者 田艺超 郭春岚 +7 位作者 李珍 尤欣 刘晓燕 苏金梅 赵斯佳 穆月 孙伟 李倩 《Chinese Medical Sciences Journal》 CAS CSCD 2024年第1期19-28,I0003,共11页
目的唾液腺是原发性干燥综合征(pSS)的主要靶器官,因此唾液被认为是腺体病理生理学和疾病状态的镜子。本研究旨在说明pSS患者的唾液蛋白质组学特征,并鉴定可能辅助诊断的潜在生物标志物。方法发现集包含49个样本[24个来自pSS,25个来自... 目的唾液腺是原发性干燥综合征(pSS)的主要靶器官,因此唾液被认为是腺体病理生理学和疾病状态的镜子。本研究旨在说明pSS患者的唾液蛋白质组学特征,并鉴定可能辅助诊断的潜在生物标志物。方法发现集包含49个样本[24个来自pSS,25个来自年龄和性别匹配的健康对照(HCs)],验证集包括25个样本(12个来自pSS,13个来自HCs)。36例pSS患者和38例健康对照者以2:1的比例集中随机分配至Discovery组或验证组。在2D LC-HRMS/MS平台上使用数据非依赖性采集(DIA)策略分析来自pSS患者和健康对照组的未刺激性全唾液样本,以揭示差异蛋白。根据基因本体(GO)分析和国际药学文摘(IPA)分析的蛋白质注释,使用DIA分析验证了关键蛋白质。随机森林用于建立SS的预测模型。结果共发现1,963个蛋白,其中136个蛋白在pSS患者中表现出差异性。生物信息学研究表明这些蛋白质主要与免疫功能、新陈代谢和炎症有关。一组19个蛋白质生物标志物通过基于P值和随机森林的排序顺序进行鉴定,并验证为具有特殊曲线下面积(AUC)值(发现集:0.817;验证集:0.882)的潜在生物标志物,可用于鉴别pSS患者和健康对照人群。结论新发现的候选蛋白组合可能有助于pSS的诊断。唾液蛋白质组学分析是一种很有前途的无创方法,可用于对pSS患者进行预后评估以及早期和精确治疗。DIA具备最佳的时间效率和数据可靠性,可望成为未来唾液蛋白质组研究的合适选择。 展开更多
关键词 原发性干燥综合征 唾液 蛋白质组学分析 质谱 诊断
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Characterization of peach tree crown by using high-resolution images from an unmanned aerial vehicle 被引量:12
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作者 yue mu Yuichiro Fujii +5 位作者 Daisuke Takata Bangyou Zheng Koji Noshita Kiyoshi Honda Seishi Ninomiya Wei Guo 《Horticulture Research》 SCIE 2018年第1期22-31,共10页
In orchards, measuring crown characteristics is essential for monitoring the dynamics of tree growth and optimizing farm management. However, it lacks a rapid and reliable method of extracting the features of trees wi... In orchards, measuring crown characteristics is essential for monitoring the dynamics of tree growth and optimizing farm management. However, it lacks a rapid and reliable method of extracting the features of trees with an irregular crown shape such as trained peach trees. Here, we propose an efficient method of segmenting the individual trees and measuring the crown width and crown projection area (CPA) of peach trees with time-series information, based on gathered images. The images of peach trees were collected by unmanned aerial vehicles in an orchard in Okayama, Japan, and then the digital surface model was generated by using a Structure from Motion (SfM) and Multi-View Stereo (MVS) based software. After individual trees were identified through the use of an adaptive threshold and marker-controlled watershed segmentation in the digital surface model, the crown widths and CPA were calculated, and the accuracy was evaluated against manual delineation and field measurement, respectively. Taking manual delineation of 12 trees as reference, the root-mean-square errors of the proposed method were 0.08 m (R^(2) = 0.99) and 0.15 m (R^(2) = 0.93) for the two orthogonal crown widths, and 3.87 m2 for CPA (R^(2) = 0.89), while those taking field measurement of 44 trees as reference were 0.47 m (R^(2) = 0.91), 0.51 m (R^(2) = 0.74), and 4.96 m2 (R^(2) = 0.88). The change of growth rate of CPA showed that the peach trees grew faster from May to July than from July to September, with a wide variation in relative growth rates among trees. Not only can this method save labour by replacing field measurement, but also it can allow farmers to monitor the growth of orchard trees dynamically. 展开更多
关键词 CROWN TREE WATERSHED
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多级中空纳米纤维二次电池电极材料 被引量:1
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作者 高松伟 郭威 +6 位作者 牟粤 朱柯平 崔志民 满兴坤 张千帆 王女 赵勇 《科学通报》 EI CAS CSCD 北大核心 2024年第16期2157-2173,共17页
多级中空纳米纤维材料具有结构可控、成分可调的优点,在二次电池电极材料领域应用广泛.在结构方面:多级中空结构可以有效缓冲电极材料在电化学反应离子嵌/脱过程中的体积变化,阻止电极材料粉碎、脱落,增加电解液和电极材料的有效接触面... 多级中空纳米纤维材料具有结构可控、成分可调的优点,在二次电池电极材料领域应用广泛.在结构方面:多级中空结构可以有效缓冲电极材料在电化学反应离子嵌/脱过程中的体积变化,阻止电极材料粉碎、脱落,增加电解液和电极材料的有效接触面积,缩短离子/电子传输路径;在成分方面:可以实现不同特性材料的合理耦合,提升电极材料电导率,加速氧化还原反应动力学.多级中空纳米纤维结构和成分的协同增强作用在提升二次电池容量、倍率、循环性能方面效果显著.本文归纳了现阶段制备多级中空结构纳米纤维的几类方法,包括单针头静电纺丝、多流体静电纺丝和其他合成方法(模板法、水热法、自组装法等).随后,总结了不同结构、成分的纤维在二次电池(如锂、钠、钾离子电池,锂/钠-硫电池,锂金属-空气电池,超级电容器等)中的应用进展.最后,探讨了多级中空结构纳米纤维材料在电化学储能领域的应用潜力. 展开更多
关键词 多级中空结构 纳米纤维 异质结构 二次电池 电极材料
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Analyzing Nitrogen Effects on Rice Panicle Development by Panicle Detection and Time-Series Tracking 被引量:2
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作者 Qinyang Zhou Wei Guo +5 位作者 Na Chen Ze Wang Ganghua Li Yanfeng Ding Seishi Ninomiya yue mu 《Plant Phenomics》 SCIE EI CSCD 2023年第2期253-267,共15页
Detailed observation of the phenotypic changes in rice panicle substantially helps us to understand the yield formation.In recent studies,phenotyping of rice panicles during the heading–flowering stage still lacks co... Detailed observation of the phenotypic changes in rice panicle substantially helps us to understand the yield formation.In recent studies,phenotyping of rice panicles during the heading–flowering stage still lacks comprehensive analysis,especially of panicle development under different nitrogen treatments.In this work,we proposed a pipeline to automatically acquire the detailed panicle traits based on time-series images by using the YOLO v5,ResNet50,and DeepSORT models.Combined with field observation data,the proposed method was used to test whether it has an ability to identify subtle differences in panicle developments under different nitrogen treatments.The result shows that panicle counting throughout the heading–flowering stage achieved high accuracy(R^(2)=0.96 and RMSE=1.73),and heading date was estimated with an absolute error of 0.25 days.In addition,by identical panicle tracking based on the time-series images,we analyzed detailed flowering phenotypic changes of a single panicle,such as flowering duration and individual panicle flowering time.For rice population,with an increase in the nitrogen application:panicle number increased,heading date changed little,but the duration was slightly extended;cumulative flowering panicle number increased,rice flowering initiation date arrived earlier while the ending date was later;thus,the flowering duration became longer.For a single panicle,identical panicle tracking revealed that higher nitrogen application led to earlier flowering initiation date,significantly longer flowering days,and significantly longer total duration from vigorous flowering beginning to the end(total DBE).However,the vigorous flowering beginning time showed no significant differences and there was a slight decrease in daily DBE. 展开更多
关键词 HEADING identical FLOWERING
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Global sources, emissions, transport and deposition of dust and sand and their effects on the climate and environment: a review 被引量:6
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作者 Feng Wang Xueqiu Zhao +3 位作者 Cynthia Gerlein-Safdi yue mu Dongfang Wang Qi Lu 《Frontiers of Environmental Science & Engineering》 SCIE EI CAS CSCD 2017年第1期35-43,共9页
Dust and Sand Storms (DSS) originating in deserts in arid and semi-arid regions are events raising global public concern. An important component of atmospheric aerosols, dust aerosols play a key role in climatic and... Dust and Sand Storms (DSS) originating in deserts in arid and semi-arid regions are events raising global public concern. An important component of atmospheric aerosols, dust aerosols play a key role in climatic and environmental changes at the regional and the global scale. Deserts and semi-deserts are the main source of dust and sand, but regions that undergo vegetation deterioration and desertification due to climate change and human activities also contribute significantly to DSS. Dust aerosols are mainly composed of dust particles with an average diameter of 2 l.tm, which can be transported over thousands of kilometers. Dust aerosols influence the radiation budget of the earth- atmosphere system by scattering solar short-wave radiation and absorbing surface long-wave radiation. They can also change albedo and rainfall patterns because they can act as cloud condensation nuclei (CCN) or ice nuclei (IN). Dust deposition is an important source of both marine nutrients and contaminants. Dust aerosols that enter marine ecosystems after long-distance transport influence phytoplankton biomass in the oceans, and thus global climate by altering the amount of CO2 absorbed by phytoplankton. In addition, the carbonates carried by dust aerosols are an important source of carbon for the alkaline carbon pool, which can buffer atmospheric acidity and increase the alkalinity of seawater. DSS have both positive and negative impacts on human society: they can exert adverse impacts on human's living environment, but can also contribute to the mitigation of global warming and the reduction of atmospheric acidity. 展开更多
关键词 Dust and sand storm Climate effects Radiative forcing Cloud condensation nuclei PRECIPITATION Iron fertilizer
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Easy MPE:Extraction of Quality Microplot Images for UAV-Based High-Throughput Field Phenotyping 被引量:3
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作者 Léa Tresch yue mu +5 位作者 Atsushi Itoh Akito Kaga Kazunori Taguchi Masayuki Hirafuji Seishi Ninomiya Wei Guo 《Plant Phenomics》 2019年第1期30-38,共9页
Microplot extraction(PE)is a necessary image processing step in unmanned aerial vehicle-(UAV-)based research on breeding fields.At present,it is manually using ArcGIS,QGIS,or other GIS-based software,but achieving the... Microplot extraction(PE)is a necessary image processing step in unmanned aerial vehicle-(UAV-)based research on breeding fields.At present,it is manually using ArcGIS,QGIS,or other GIS-based software,but achieving the desired accuracy is timeconsuming.We therefore developed an intuitive,easy-to-use semiautomatic program for MPE called Easy MPE to enable researchers and others to access reliable plot data UAV images of whole fields under variable field conditions.The program uses four major steps:(1)binary segmentation,(2)microplot extraction,(3)production of∗.shp files to enable further file manipulation,and(4)projection of individual microplots generated from the orthomosaic back onto the raw aerial UAV images to preserve the image quality.Crop rows were successfully identified in all trial fields.The performance of the proposed method was evaluated by calculating the intersection-over-union(IOU)ratio between microplots determined manually and by Easy MPE:the average IOU(±SD)of all trials was 91%(±3). 展开更多
关键词 enable image UNION
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