Development of the SCNT Embryo from Butyrolactone Ⅰ Prematured Bovine Oocytes

The somatic cell nuclear transfer （SCNT） technique has been applied successfully in a range of mammalian species giving rise to offspring, however, the efficiency of development to term has remained low. Oocyte meiotic arrest is an important option to make the SCNT more flexible and increase the number of cloned embryos produced. This paper showed that the use of butyrolactone I to arrest the meiotic division for 24 h prior to in vitro maturation （IVM） provided bovine oocytes capability of supporting development of blastocysts as efficiently as non arrested oocytes. In the SCNT group, cleavage rates were not affected by prematuration when adding butyrolactone I during IVM （57.9% vs. 62.4%; control vs. 10 μmol.L^-1 butyrolactone I）, developmental rates to biastocyst were unaffected （22.0% vs. 20.3%; control vs. 10 μmol.L^-1 butyrolactone I, p〉0.05） by the addition ofbutyrolactone I during IVM in cloned embryos. Moreover, the total cell numbers of blastocyst were not affected by butyrolactone I （total nucleus numbers, 132±16.5 vs. 128±19.4, p〉0.05）. In conclusion, the SCNT embryos from butyrolactone I- prematured bovine oocytes had the same developmental potential as the non treated one. The present study provided a method for laboratories of in vitro embryos production, mainly those working on the SCNT, where prematuration could be used to iiacrease the flexibility of the procedure.

肉碱对液态保存猪精子质量和受精能力的影响

探究肉碱对液态保存猪精子质量的影响,主要检测参数包括猪精子的活力、质膜完整性、抗氧化性(总抗氧化活力、活性氧含量和脂质过氧化水平)、ATP含量、线粒体活性和体外受精能力。结果显示,在保存液中添加肉碱可以有效维持猪精子的质量,如精子的活力和质膜完整性。同时发现,肉碱主要通过提高猪精子的抗氧化活力、提高ATP含量以及线粒体活性来维持猪精子质量。此外,本试验通过检测肉碱对精子受精能力和发育潜能的影响发现,添加肉碱可以增加精子结合到透明带的比例。结果表明,在精液保存液中适当添加肉碱可以改善在17℃液态保存的猪精子的质量。

精胺在猪精液液态保存过程中的作用

目前液态保存精液的人工授精技术被猪场广泛采用,因此廉价有效的保存液对生猪生产十分重要。本研究的目的是探究精胺对液态保存猪精子质量的影响。主要检测了精子的活力、质膜完整性、顶体完整性、活性氧含量、线粒体膜电位、细胞凋亡水平和体外受精能力。结果显示,精液保存液中添加精胺有助于提高猪精子保存质量,同时发现精胺可以减少活性氧的过多产生和抑制精子凋亡。此外,本研究发现精胺能够提高精子受精能力和囊胚发育能力。结果表明,在精液保存液中添加适当浓度的精胺有助于改善在液态保存的猪精子的质量。