Effect of local ozone treatment on inflammatory cytokine, growth cytokine and apoptosis molecule expression in anal fistula wound

Objective:To study the effect of local ozone therapy on inflammatory cytokine, growth cytokine and apoptosis molecule expression in anal fistula wound.Methods: Patients with low simple anal fistula who received anal fistulectomy in Eighth Hospital of Wuhan between June 2014 and December 2016 were selected and randomly divided into ozone group and control group who received ozone combined with routine treatment and routine treatment respectively. 5 d, 10 d and 15 d after treatment, proper amount of wound granulation tissue was clamped to determine the expression of inflammatory cytokines IFN-γ, TNF-α, IL-1, IL-8 and IL-12, growth cytokines VEGF, FGF-1, FGF-2 and EGF as well as apoptosis molecules Bax, P53 and caspase-3.Results:5 d, 10 d and 15 d after treatment, IFN-γ, TNF-α, IL-1, IL-8, IL-12, VEGF, FGF-1, FGF-2 and EGF protein expression in anal fistula wound of ozone group were significantly higher than those of control group while Bax, P53 and caspase-3 protein expression were significantly lower than those of control group.Conclusion: Local ozone treatment can increase the expression of inflammatory cytokines and growth cytokines and inhibit the expression of apoptosis molecules in anal fistula wound, and it is conducive to wound healing.

Effect of calcium alginate dressing on the cytokine contents,collagen synthesis-degradation balance and apoptosis gene expression in the wound after perianal abscess surgery

Objective: To study the effect of calcium alginate dressing on the cytokine contents, collagen synthesis - degradation balance and apoptosis gene expression in the wound after perianal abscess surgery. Methods: Patients with perianal abscess who received surgical resection in the Eighth Hospital of Wuhan between May 2014 and February 2017 were selected and randomly divided into the group A who received calcium alginate dressing combined with kangfuxin solution and recombinant human epidermal growth factor for dressing change and the group B who received kangfuxin solution and recombinant human epidermal growth factor for dressing change. 3 d, 6 d and 9 d after dressing change, appropriate amount of wound tissue was collected to determine the expression of cytokines, collagen metabolites and apoptosis genes. Results: 3 d, 6 d and 9 d after dressing change, TGF-β1, Smad3, EGF and bFGF protein expression as well as Col-I, Col-II, Col-III, TIMP1 and TIMP2 protein expression in wounds of both groups of patients were increasing while Fas, FasL, Bax and Caspase-3 protein expression were decreasing, and TGF-β1, Smad3, EGF and bFGF protein expression as well as Col-I, Col-II, Col-III, TIMP1 and TIMP2 protein expression in wounds of group A were significantly higher than those of group B while Fas, FasL, Bax and Caspase-3 protein expression were significantly lower than those of group B. Conclusion: Calcium alginate dressing for wound dressing after perianal abscess surgery an increase the pro-proliferation cytokine expression, adjust the collagen synthesis - degradation balance and inhibit apoptosis, and it is conducive to wound healing.