BACKGROUND Single nucleotide polymorphisms(SNPs)are universally present in nucleotide excision repair(NER)pathway genes,which could make impacts on colorectal carcinogenesis and prognosis.AIM To explore the associatio...BACKGROUND Single nucleotide polymorphisms(SNPs)are universally present in nucleotide excision repair(NER)pathway genes,which could make impacts on colorectal carcinogenesis and prognosis.AIM To explore the association of all tagSNPs in NER pathway genes with colorectal cancer(CRC)risk and prognosis in a northern Chinese population by a two-stage case-control design composed of a discovery and validation stage.METHODS Genotyping for NER SNPs was performed using kompetitive allele specific PCR.In the discovery stage,39 tagSNPs in eight genes were genotyped in 368 subjects,including 184 CRC cases and 184 individual-matched controls.In the validation stage,13 SNPs in six genes were analyzed in a total of 1712 subjects,including 854 CRC cases and 858 CRC-free controls.RESULTS Two SNPs(XPA rs10817938 and XPC rs2607775)were associated with an increased CRC risk in overall and stratification analyses.Significant cumulative and interaction effects were also demonstrated in the studied SNPs on CRC risk.Another two SNPs(ERCC2 rs1052555 and ERCC5 rs2228959)were newly found to be associated with a poor overall survival of CRC patients.CONCLUSION Our findings suggest novel SNPs in NER pathway genes that can be predictive for CRC risk and prognosis in a large-scale Chinese population.The present study has referential values for the identification of all-round NER-based genetic biomarkers in predicting the susceptibility and clinical outcome of CRC.展开更多
Bottom simulating reflector(BSR)has been recognized as one of the indicators of gas hydrates.However,BSR and hydrate are not one-to-one correspondence.In the Xisha area of South China Sea(SCS),carbonate rocks wildly d...Bottom simulating reflector(BSR)has been recognized as one of the indicators of gas hydrates.However,BSR and hydrate are not one-to-one correspondence.In the Xisha area of South China Sea(SCS),carbonate rocks wildly develop,which continuously distribute parallel to the seafloor with high amplitude on seismic sections,exhibiting reflections similar to BSRs in the Shenhu area nearby.This phenomenon causes some interference to hydrates identification.In this paper,the authors discussed the typical geophysical differences between carbonate rocks and hydrates,indicating that the main difference exists in relationship between porosity and velocity,causing different amplitude versus offset(AVO)characters.Then the authors proposed a new model assuming that the carbonates form the matrix and the hydrate fill the pore as a part of the matrix.The key modeling parameters have been optimized constrained by Pvelocities and S-velocities simultaneously,and the model works well both for carbonate rock and gas hydrate bearing sediments.For quantitative identification,the authors calculated the velocities when carbonates and hydrates form the matrix together in different proportions.Then they proposed a carbonate and hydrate identification template(CHIT),in which the possible hydrate saturation(PHS)and possible carbonate content(PCC)can be both scaled out for a group of sample composed by P-velocity and S-velocity.If PHS is far larger than PCC,it is more likely to be a hydrate sample because carbonates and hydrates do not coexist normally.The real data application shows that the template can effectively distinguish between hydrates and carbonate rocks,consequently reducing the risk of hydrate exploration.展开更多
Salmonella Aberdeen is a ubiquitous pathogen that can cause severe gastroenteritis and plays an important role in food-borne illness and international food-trade dispute.[1]However,the molecular biological characteris...Salmonella Aberdeen is a ubiquitous pathogen that can cause severe gastroenteritis and plays an important role in food-borne illness and international food-trade dispute.[1]However,the molecular biological characteristics of this organism have been rarely studied.This study was conducted in accordance with the Declaration of Helsinki and aimed to assess the epidemiological and clinical characteristics of a food-borne outbreak caused by Salmonella Aberdeen.Furthermore,the molecular biological characteristics of the outbreak isolates were detected and analyzed.From June 10 to 12,2018,an enteritis outbreak affected 75 employees in the Fushan district of Yantai,Shandong province,China,including 33 males and 42 females.The mean age of the patients was 34.2±5.3 years.展开更多
Dear Editor, Trichomonas vaginalis ( T. vaginalis), a flagellated protozoan parasite emerged as one of the most common nonviral sexually transmitted infections worldwide, often inhabits the vagina, urethra, prostat...Dear Editor, Trichomonas vaginalis ( T. vaginalis), a flagellated protozoan parasite emerged as one of the most common nonviral sexually transmitted infections worldwide, often inhabits the vagina, urethra, prostate, and epididymis.X It has been estimated that there are more than 170 million new cases of T. vaginalis infections per year worldwide. However, current knowledge of T. vaginalis and trichomoniasis is based mainly on studies in female vaginal infections. The prevalence of trichomoniasis in males is far less well characterized than that in females, probably because the infection seems to be asymptomatic in most men and can be resolved after treatment with one dose of metronidazole.展开更多
基金Supported by the National Key R&D Program of China,No.2018YFC1311600
文摘BACKGROUND Single nucleotide polymorphisms(SNPs)are universally present in nucleotide excision repair(NER)pathway genes,which could make impacts on colorectal carcinogenesis and prognosis.AIM To explore the association of all tagSNPs in NER pathway genes with colorectal cancer(CRC)risk and prognosis in a northern Chinese population by a two-stage case-control design composed of a discovery and validation stage.METHODS Genotyping for NER SNPs was performed using kompetitive allele specific PCR.In the discovery stage,39 tagSNPs in eight genes were genotyped in 368 subjects,including 184 CRC cases and 184 individual-matched controls.In the validation stage,13 SNPs in six genes were analyzed in a total of 1712 subjects,including 854 CRC cases and 858 CRC-free controls.RESULTS Two SNPs(XPA rs10817938 and XPC rs2607775)were associated with an increased CRC risk in overall and stratification analyses.Significant cumulative and interaction effects were also demonstrated in the studied SNPs on CRC risk.Another two SNPs(ERCC2 rs1052555 and ERCC5 rs2228959)were newly found to be associated with a poor overall survival of CRC patients.CONCLUSION Our findings suggest novel SNPs in NER pathway genes that can be predictive for CRC risk and prognosis in a large-scale Chinese population.The present study has referential values for the identification of all-round NER-based genetic biomarkers in predicting the susceptibility and clinical outcome of CRC.
基金the China Geological Survey Program(DD20190217)2018 Open Fund Project of Key Laboratory of Submarine Mineral Resources,Ministry of Natural Resources(KLMMR-2018-A-04)Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory(GML2019ZD0102)
文摘Bottom simulating reflector(BSR)has been recognized as one of the indicators of gas hydrates.However,BSR and hydrate are not one-to-one correspondence.In the Xisha area of South China Sea(SCS),carbonate rocks wildly develop,which continuously distribute parallel to the seafloor with high amplitude on seismic sections,exhibiting reflections similar to BSRs in the Shenhu area nearby.This phenomenon causes some interference to hydrates identification.In this paper,the authors discussed the typical geophysical differences between carbonate rocks and hydrates,indicating that the main difference exists in relationship between porosity and velocity,causing different amplitude versus offset(AVO)characters.Then the authors proposed a new model assuming that the carbonates form the matrix and the hydrate fill the pore as a part of the matrix.The key modeling parameters have been optimized constrained by Pvelocities and S-velocities simultaneously,and the model works well both for carbonate rock and gas hydrate bearing sediments.For quantitative identification,the authors calculated the velocities when carbonates and hydrates form the matrix together in different proportions.Then they proposed a carbonate and hydrate identification template(CHIT),in which the possible hydrate saturation(PHS)and possible carbonate content(PCC)can be both scaled out for a group of sample composed by P-velocity and S-velocity.If PHS is far larger than PCC,it is more likely to be a hydrate sample because carbonates and hydrates do not coexist normally.The real data application shows that the template can effectively distinguish between hydrates and carbonate rocks,consequently reducing the risk of hydrate exploration.
基金Project supported by the National Natural Science Foundation of China(Nos.81701524,81671512,and 81701428)the Frontier Technology Project of Shanghai(No.SHDC12015122),China
文摘Salmonella Aberdeen is a ubiquitous pathogen that can cause severe gastroenteritis and plays an important role in food-borne illness and international food-trade dispute.[1]However,the molecular biological characteristics of this organism have been rarely studied.This study was conducted in accordance with the Declaration of Helsinki and aimed to assess the epidemiological and clinical characteristics of a food-borne outbreak caused by Salmonella Aberdeen.Furthermore,the molecular biological characteristics of the outbreak isolates were detected and analyzed.From June 10 to 12,2018,an enteritis outbreak affected 75 employees in the Fushan district of Yantai,Shandong province,China,including 33 males and 42 females.The mean age of the patients was 34.2±5.3 years.
基金This study was supported by a key grant from the Joint Research Project for Emerging Frontier (SHDC 12015122), Shanghai Shenkang Hospital Applicable Technology Project, the National Key Basic Research Program of China (2015AA020404) and the National Nature Science Foundation of China (81671512).
文摘Dear Editor, Trichomonas vaginalis ( T. vaginalis), a flagellated protozoan parasite emerged as one of the most common nonviral sexually transmitted infections worldwide, often inhabits the vagina, urethra, prostate, and epididymis.X It has been estimated that there are more than 170 million new cases of T. vaginalis infections per year worldwide. However, current knowledge of T. vaginalis and trichomoniasis is based mainly on studies in female vaginal infections. The prevalence of trichomoniasis in males is far less well characterized than that in females, probably because the infection seems to be asymptomatic in most men and can be resolved after treatment with one dose of metronidazole.