Background： Ventilator-induced lung injury （VILI） is commonly associated with barrier dysfunction and inflammation reaction. Glutamine could ameliorate VILI, but its role has not been fully elucidated, This study e...Background： Ventilator-induced lung injury （VILI） is commonly associated with barrier dysfunction and inflammation reaction. Glutamine could ameliorate VILI, but its role has not been fully elucidated, This study examined the relationship between inflammatory cytokines （interleukin JILl-6, tumor necrosis factor [TNF]-α, and IL-10） and adherens junctions （E-cadherin, p 120-catenin）, which were ameliorated by glutamine in VILI, both in vitro and in vivo. Methods： For the in vivo study, 30 healthy C57BL/6 mice weighing 25-30 g were randomly divided into five groups with random number table （n = 6 in each group）： control （Group C）; low tidal volume （Group L）; low tidal volume ＋ glutamine （Group L ＋ G）; high tidal volume （Group H）; and high tidal volume ＋ glutamine （Group H ＋ G）. Mice in all groups, except Group C, underwent mechanical ventilation for 4 h. For the in vitro study, mouse lung epithelial 12 （MLE- 12） cells pretreated with glutamine underwent cyclic stretching at 20% for 4 h. Cell lysate and lung tissue were obtained to detect the junction proteins, inflammatory cytokines, and lung pathological changes by the Western blotting, cytokine assay, hematoxylin and eosin staining, and immunofluorescence. Results： In vivo, compared with Group C, total cell counts （t= -28.182, P 〈 0.01）, the percentage of neutrophils （t = -28.095, P 〈 0.01）, IL-6 （t = -28.296, P 〈 0.01 ）, and TNF-α（t = - 19.812, P 〈 0.01 ） in bronchoalveolar lavage （BAL） fluid, lung injury scores （t = -6.708, P 〈 0.01）, and the wet-to-dry ratio （t = - 15.595, P 〈 0.01 ） were increased in Group H; IL- 10 in BAL fluid （t = 9.093, P 〈 0.01 ） and the expression of E-cadherin （t= 10.044, P 〈 0.01） and p120-catenin （t = 13.218, P 〈 0.01） were decreased in Group H. Compared with Group H, total cell counts （t - 14.844, P 〈 0.01 ）, the percentage of neutrophils （t = 18.077, P 〈 0.0 l ）, IL-6 （t - 18.007, P 〈 0.01 ）, and TNF-α （t =1 0.171, P 〈 0.01 ） in BAL fluid were decreased in Group H ＋ G; IL-10 in BAL fluid （t - -7.531, P 〈 0.01 ） and the expression of E-cadherin （t = - 14.814, P 〈 0.01 ） and p 120-catenin （t = -9.114, P 〈 0.01 ） were increased in Group H ＋ G. In vitro, compared with the nonstretching group, the levels of IL-6 （t = 21.111, P 〈 0.01 ） and TNF-α （t - 15.270, P 〈 0.01 ） were increased in the 20% cyclic stretching group; the levels of IL- 10 （t = 5.450, P 〈 0.01 ） and the expression of E-cadherin （t = 17.736, P 〈 0.01 ） and p 120-catenin （t = 16.136, P 〈 0.01 ） were decreased in the 20% cyclic stretching group. Compared with the stretching group, the levels of IL-6 （t = 11.818, P 〈 0.01） and TNF-α （t = 8.631, P 〈 0.01 ） decreased in the glutamine group; the levels of IL- 10 （t = 3.203, P 〈 0.05） and the expression of E-cadherin （t= 13.567, P 〈 0.01） and p 120-catenin （t = -10.013, P 〈 0.01） were increased in the glutamine group. Conclusions： High tidal volume mechanical ventilation and 20% cyclic stretching could cause VIM. Glutamine regulates VIM by improving cytokines and increasing the adherens junctions, protein E-cadherin and p 120-catenin, to enhance the epithelial barrier function.展开更多
基金This work was supported by grants from the National Natural Science Foundation of China （Nos. 81570074, 81770076）.
文摘Background： Ventilator-induced lung injury （VILI） is commonly associated with barrier dysfunction and inflammation reaction. Glutamine could ameliorate VILI, but its role has not been fully elucidated, This study examined the relationship between inflammatory cytokines （interleukin JILl-6, tumor necrosis factor [TNF]-α, and IL-10） and adherens junctions （E-cadherin, p 120-catenin）, which were ameliorated by glutamine in VILI, both in vitro and in vivo. Methods： For the in vivo study, 30 healthy C57BL/6 mice weighing 25-30 g were randomly divided into five groups with random number table （n = 6 in each group）： control （Group C）; low tidal volume （Group L）; low tidal volume ＋ glutamine （Group L ＋ G）; high tidal volume （Group H）; and high tidal volume ＋ glutamine （Group H ＋ G）. Mice in all groups, except Group C, underwent mechanical ventilation for 4 h. For the in vitro study, mouse lung epithelial 12 （MLE- 12） cells pretreated with glutamine underwent cyclic stretching at 20% for 4 h. Cell lysate and lung tissue were obtained to detect the junction proteins, inflammatory cytokines, and lung pathological changes by the Western blotting, cytokine assay, hematoxylin and eosin staining, and immunofluorescence. Results： In vivo, compared with Group C, total cell counts （t= -28.182, P 〈 0.01）, the percentage of neutrophils （t = -28.095, P 〈 0.01）, IL-6 （t = -28.296, P 〈 0.01 ）, and TNF-α（t = - 19.812, P 〈 0.01 ） in bronchoalveolar lavage （BAL） fluid, lung injury scores （t = -6.708, P 〈 0.01）, and the wet-to-dry ratio （t = - 15.595, P 〈 0.01 ） were increased in Group H; IL- 10 in BAL fluid （t = 9.093, P 〈 0.01 ） and the expression of E-cadherin （t= 10.044, P 〈 0.01） and p120-catenin （t = 13.218, P 〈 0.01） were decreased in Group H. Compared with Group H, total cell counts （t - 14.844, P 〈 0.01 ）, the percentage of neutrophils （t = 18.077, P 〈 0.0 l ）, IL-6 （t - 18.007, P 〈 0.01 ）, and TNF-α （t =1 0.171, P 〈 0.01 ） in BAL fluid were decreased in Group H ＋ G; IL-10 in BAL fluid （t - -7.531, P 〈 0.01 ） and the expression of E-cadherin （t = - 14.814, P 〈 0.01 ） and p 120-catenin （t = -9.114, P 〈 0.01 ） were increased in Group H ＋ G. In vitro, compared with the nonstretching group, the levels of IL-6 （t = 21.111, P 〈 0.01 ） and TNF-α （t - 15.270, P 〈 0.01 ） were increased in the 20% cyclic stretching group; the levels of IL- 10 （t = 5.450, P 〈 0.01 ） and the expression of E-cadherin （t = 17.736, P 〈 0.01 ） and p 120-catenin （t = 16.136, P 〈 0.01 ） were decreased in the 20% cyclic stretching group. Compared with the stretching group, the levels of IL-6 （t = 11.818, P 〈 0.01） and TNF-α （t = 8.631, P 〈 0.01 ） decreased in the glutamine group; the levels of IL- 10 （t = 3.203, P 〈 0.05） and the expression of E-cadherin （t= 13.567, P 〈 0.01） and p 120-catenin （t = -10.013, P 〈 0.01） were increased in the glutamine group. Conclusions： High tidal volume mechanical ventilation and 20% cyclic stretching could cause VIM. Glutamine regulates VIM by improving cytokines and increasing the adherens junctions, protein E-cadherin and p 120-catenin, to enhance the epithelial barrier function.
