Resolving three-dimensional morphological features in thick specimens remains a significant challenge for label-free imaging.We report a new speckle diffraction tomography(SDT)approach that can image thick biological ...Resolving three-dimensional morphological features in thick specimens remains a significant challenge for label-free imaging.We report a new speckle diffraction tomography(SDT)approach that can image thick biological specimens with~500 nm lateral resolution and~1μm axial resolution in a reflection geometry.In SDT,multiple-scattering background is rejected through spatiotemporal gating provided by dynamic speckle-field interferometry,while depth-resolved refractive index maps are reconstructed by developing a comprehensive inverse-scattering model that also considers specimen-induced aberrations.Benefiting from the high-resolution and full-field quantitative imaging capabilities of SDT,we successfully imaged red blood cells and quantified their membrane fluctuations behind a turbid medium with a thickness of 2.8 scattering mean-free paths.Most importantly,we performed volumetric imaging of cornea inside an ex vivo rat eye and quantified its optical properties,including the mapping of nanoscale topographic features of Dua’s and Descemet’s membranes that had not been previously visualized.展开更多
基金S.K.,Y.L.,P.T.C.S.,and Z.Y.acknowledge support from National Institutes of Health(NIH)funding 5-P41-EB015871-27 and Hamamatsu CorporationP.T.C.S.further acknowledges support from the Singapore-Massachusetts Institute of Technology Alliance for Research and Technology(SMART)Center,Critical Analytics for Manufacturing Personalized-Medicine IRG.P.T.C.S.and Z.Y.further acknowledge support from NIH R01DA045549,R21GM140613-02,R01HL158102.R.Z.acknowledges support from Croucher Innovation Awards 2019(Grant No.CM/CT/CF/CIA/0688/19ay)+2 种基金Hong Kong Innovation and Technology Commission(ITS/148/20 and ITS/178/20FP)Hong Kong General Research Fund(14209521)The Chinese University of Hong Kong Research Sustainability of Major RGC Funding Schemes-Strategic Areas.
文摘Resolving three-dimensional morphological features in thick specimens remains a significant challenge for label-free imaging.We report a new speckle diffraction tomography(SDT)approach that can image thick biological specimens with~500 nm lateral resolution and~1μm axial resolution in a reflection geometry.In SDT,multiple-scattering background is rejected through spatiotemporal gating provided by dynamic speckle-field interferometry,while depth-resolved refractive index maps are reconstructed by developing a comprehensive inverse-scattering model that also considers specimen-induced aberrations.Benefiting from the high-resolution and full-field quantitative imaging capabilities of SDT,we successfully imaged red blood cells and quantified their membrane fluctuations behind a turbid medium with a thickness of 2.8 scattering mean-free paths.Most importantly,we performed volumetric imaging of cornea inside an ex vivo rat eye and quantified its optical properties,including the mapping of nanoscale topographic features of Dua’s and Descemet’s membranes that had not been previously visualized.
