What does 140 k versus 40 k reveal? It has been reported, using Scopus data, that the number of short papers(also known as ònon-source itemsó in the Web of Science) published in the United States in 2020 was...What does 140 k versus 40 k reveal? It has been reported, using Scopus data, that the number of short papers(also known as ònon-source itemsó in the Web of Science) published in the United States in 2020 was about 140,000(about 18.5% of its total publication).展开更多
Objective： The aim of our study was to investigate the effect of Pin 1 on the telomerase activity in human colorectal carcinoma HCT116 cells. Methods： Firstly, we transfected plasmid pGenesil-l-Pinl （p-shRNA） usin...Objective： The aim of our study was to investigate the effect of Pin 1 on the telomerase activity in human colorectal carcinoma HCT116 cells. Methods： Firstly, we transfected plasmid pGenesil-l-Pinl （p-shRNA） using liposome （Lipofectamine 2000） into colorectal cancer HCT-116 cells to down-regulate the expression of Pin1. To detect the apoptotic rate of HCT116 cells was by cytometry （FCM）. The expression of Pin1 and hTERT at RNA levels in human colorectal cancer HCT116 cells were determined by RT-PCR. To evaluate the activity of telomerase was by TRAP-silver staining. The subcellular localization and accumulative level of p-NF-κB/p65 protein at the nuclear was detected by Immunofluorescence and Western blotting. The DNA-binding activity of NF-κB/p65 was detected by electrophoretic mobility shift assay （EMSA）. Results： Using liposome into colorectal cancer HCT-116 cells, and down-regulate the expression of Pin1 （0.392 ± 0.072-fold; P = 0.001）, and the apoptotic rate was increased （11.40% ± 1.54%; P 〈 0.05）. Compared with transfected p-CON cell group, in transfected p-shRNA cell group, the transcription of hTERT was lower （0.171 ±0.060-fold; P = 0.001） by quantitative real-time RT-PCR, and the results of TRAP-silver staining analysis suggested that the telomerase activity was significantly declined （0.384 ± 0.015-fold; P 〈 0.05）. Furthermore, it was demonstrated by Immunofluorescence that p-NF-κB/p65 had a nuclear localization, and the level of p-NF-κB/p65 protein at the nuclear was reduced with silencing the expression of Pin1 by Western blotting. Using EMSA, it was suggested that NF-κB/p65 was able to bind to hTERT promoter, and the direct interaction was declined with silencing the expression of Pin1. Conclusion： Taken together, silencing Pin1 may suppress activity of telomerase and the expression of hTERT by inhibiting NF-κB/p65 activity and reducing the combination of NF-κB/p65 and hTERT gene promoter.展开更多
In this work,a good combination of strength and ductility is achieved in a Mg-13Gd-0.2Ni alloy by conventional extrusion and following aging treatment.The aged Mg-13Gd-0.2Ni alloy exhibits a yield strength(YS)of 363 M...In this work,a good combination of strength and ductility is achieved in a Mg-13Gd-0.2Ni alloy by conventional extrusion and following aging treatment.The aged Mg-13Gd-0.2Ni alloy exhibits a yield strength(YS)of 363 MPa,an ultimate tensile strength(UTS)of 433 MPa,and an elongation of 11.9%.The aged Mg-13Gd-0.2Ni alloy contains a microstructure with a 95%proportion of dynamically recrystallized(DRXed)grains with a micron size,weak texture,and a high number density of prismaticβ′precipitates within grains.The bulk compounds enriched with Ni element which are mainly formed during casting and stable in the extruded and aged samples.Few dynamic compounds are formed during extrusion,and a high density of prismaticβ′precipitates are formed during aging.The high density of prismaticβ′precipitates results in a significant increase in the strength of the Mg-13Gd-0.2Ni alloy,and the YS and UTS are increased by 153 and 136 MPa,respectively.The high proportion of DRXed grains with a weak texture contributes mainly to the high ductility and the fine compounds with a low density at grain boundaries formed during aging have no significant adverse effect on ductility of the aged Mg-13Gd-0.2Ni alloy.These findings for the novel Mg-Gd-Ni alloy can provide guidance for the design of wrought Mg alloys with superior mechanical properties.展开更多
文摘What does 140 k versus 40 k reveal? It has been reported, using Scopus data, that the number of short papers(also known as ònon-source itemsó in the Web of Science) published in the United States in 2020 was about 140,000(about 18.5% of its total publication).
基金Supported by a grant from the Natural Science Foundation of Shanxi Province(No.2010011047-1,2011021035-1)
文摘Objective： The aim of our study was to investigate the effect of Pin 1 on the telomerase activity in human colorectal carcinoma HCT116 cells. Methods： Firstly, we transfected plasmid pGenesil-l-Pinl （p-shRNA） using liposome （Lipofectamine 2000） into colorectal cancer HCT-116 cells to down-regulate the expression of Pin1. To detect the apoptotic rate of HCT116 cells was by cytometry （FCM）. The expression of Pin1 and hTERT at RNA levels in human colorectal cancer HCT116 cells were determined by RT-PCR. To evaluate the activity of telomerase was by TRAP-silver staining. The subcellular localization and accumulative level of p-NF-κB/p65 protein at the nuclear was detected by Immunofluorescence and Western blotting. The DNA-binding activity of NF-κB/p65 was detected by electrophoretic mobility shift assay （EMSA）. Results： Using liposome into colorectal cancer HCT-116 cells, and down-regulate the expression of Pin1 （0.392 ± 0.072-fold; P = 0.001）, and the apoptotic rate was increased （11.40% ± 1.54%; P 〈 0.05）. Compared with transfected p-CON cell group, in transfected p-shRNA cell group, the transcription of hTERT was lower （0.171 ±0.060-fold; P = 0.001） by quantitative real-time RT-PCR, and the results of TRAP-silver staining analysis suggested that the telomerase activity was significantly declined （0.384 ± 0.015-fold; P 〈 0.05）. Furthermore, it was demonstrated by Immunofluorescence that p-NF-κB/p65 had a nuclear localization, and the level of p-NF-κB/p65 protein at the nuclear was reduced with silencing the expression of Pin1 by Western blotting. Using EMSA, it was suggested that NF-κB/p65 was able to bind to hTERT promoter, and the direct interaction was declined with silencing the expression of Pin1. Conclusion： Taken together, silencing Pin1 may suppress activity of telomerase and the expression of hTERT by inhibiting NF-κB/p65 activity and reducing the combination of NF-κB/p65 and hTERT gene promoter.
基金supported by the National Natural Science Foundation of China(Grant Nos.52171121,51971151,52201132,and 52201131)the Natural Science Foundation of Liaoning Province of China(2022-NLTS-18-01).
文摘In this work,a good combination of strength and ductility is achieved in a Mg-13Gd-0.2Ni alloy by conventional extrusion and following aging treatment.The aged Mg-13Gd-0.2Ni alloy exhibits a yield strength(YS)of 363 MPa,an ultimate tensile strength(UTS)of 433 MPa,and an elongation of 11.9%.The aged Mg-13Gd-0.2Ni alloy contains a microstructure with a 95%proportion of dynamically recrystallized(DRXed)grains with a micron size,weak texture,and a high number density of prismaticβ′precipitates within grains.The bulk compounds enriched with Ni element which are mainly formed during casting and stable in the extruded and aged samples.Few dynamic compounds are formed during extrusion,and a high density of prismaticβ′precipitates are formed during aging.The high density of prismaticβ′precipitates results in a significant increase in the strength of the Mg-13Gd-0.2Ni alloy,and the YS and UTS are increased by 153 and 136 MPa,respectively.The high proportion of DRXed grains with a weak texture contributes mainly to the high ductility and the fine compounds with a low density at grain boundaries formed during aging have no significant adverse effect on ductility of the aged Mg-13Gd-0.2Ni alloy.These findings for the novel Mg-Gd-Ni alloy can provide guidance for the design of wrought Mg alloys with superior mechanical properties.
