Review of Factors Influencing Active Components of Lonicera japonica Thunb.

Lonicera japonica Thunb. is a typical medicinal and edible plant in China. It has antibacterial, anti-oxidant, anti-endotoxin, and anti-inflammatory effects, etc. Its pharmacological action is closely related to its active components. Based on the results of previous studies, this paper summarized the effects of variety, origin, cultivation conditions, pruning, harvesting period and processing methods on the active components of L. japonica , in the hope of providing theoretical guidance for high-yield and high-quality cultivation of L. japonica.

Content Determination of Chlorogenic Acid and Luteoloside in Flos Lonicerae

The contents of chlorogenic acid and luteoloside in Flos Lonicerae from Binhai New Area and Jinnan District of Tianjin were determined to provide basis for the quality identification of this medicinal material. The content of chlorogenic acid was determined by HPLC. In Flos Lonicerae from Binhai New Area and in Flos Lonicerae harvested at different stages from Jinnan District,the contents of chlorogenic acid were 3. 804%,5. 507%( three green stage),4. 855%( silver flower stage) and 4. 220%( golden flower stage),respectively,and the contents of luteoloside were 5. 53%,12. 405%( three green stage),14. 370%( silver flower stage) and 0. 917%( golden flower stage),respectively. The contents of chlorogenic acid in Flos Lonicerae from Jinnan District were higher than that from Binhai New Area. Among different stages,the content of chlorogenic acid was highest in three green stage,followed by that in silver flower stage. As the flowers bloomed,the content of chlorogenic acid in the medicinal material showed a significant downward trend. In Flos Lonicerae from Jinnan District,the content of luteoloside was highest in silver flower stage and lowest in golden flower stage.

Cinnamaldehyde targets SarA to enhance β-lactam antibiotic activity against methicillin-resistant Staphylococcus aureus

Methicillin-resistant Staphylococcus aureus(MRSA)is a current global public health problem due to its increasing resistance to the most recent antibiotic therapies.One critical approach is to develop ways to revitalize existing antibiotics.Here,we show that the phytogenic compound cinnamaldehyde(CIN)and β-lactam antibiotic combinations can functionally synergize and resensitize clinical MRSA isolates to β-lactam therapy and inhibit MRSA biofilm formation.Mechanistic studies indicated that the CIN potentiation effect on β-lactams was primarily the result of inhibition of the mecA expression by targeting the staphylococcal accessory regulator sarA.CIN alone or in combination with β-lactams decreased sarA gene expression and increased SarA protein phosphorylation that impaired SarA binding to the mecA promoter element and downregulated virulence genes such as those encoding biofilm,α-hemolysin,and adhesin.Perturbation of SarA-mecA binding thus interfered with PBP2a biosynthesis and this decreased MRSA resistance to β-lactams.Furthermore,CIN fully restored the anti-MRSA activities of β-lactam antibiotics in vivo in murine models of bacteremia and biofilm infections.Together,our results indicated that CIN acts as a β-lactam adjuvant and can be applied as an alternative therapy to combat multidrug-resistant MRSA infections.

Triboelectric-electromagnetic hybrid generator with swing-blade structures for effectively harvesting distributed wind energy in urban environments

The wind energy in cities cannot be exploited effectively because natural wind is unstable and complex.Therefore,a triboelectricelectromagnetic hybrid generator with swing-blade structures(SBS-TEHG)was designed to effectively harvest intermittent and continuous wind energy in an urban environment.First,the spring structure and base were considered to realize the maximum output performance of triboelectric nanogenerators.Then,the computational fluid dynamics method was applied to optimize the structure of the SBS-TEHG to improve its aerodynamic performance.The starting wind speed of the SBS-TEHG was 2 m/s,and its energy conversion efficiency was 9.04%,159%higher than that of the SBS-TEHG without guide plates at 4 m/s.The results demonstrated that the SBS-TEHG lit 105 light-emitting diodes(LEDs)under the intermittent-wind harvesting mode at a wind frequency of 1 Hz when the single swing blade operated,while a wireless PM_(2.5)&PM_(10)sensor was powered by the SBS-TEHG after a period of operation under the continuous-wind harvesting mode.The findings of this study provide a novel solution for lowspeed wind energy harvesting in cities and demonstrate the potential of SBS-TEHG as a distributed energy source.

An isolated SNM model for high-stability multi-port register file in 65 nm CMOS

In modern microprocessors, the multi-port register file is one of the key modules which provides fast and multiple data access for instructions. As the number of access ports in register files increases, stability becomes a key issue due to the voltage fluctuation on bit lines. We propose to apply an isolated inverter to address the voltage fluctuation. To assess the register stability, we derive a closed-form expression of static noise margin （SNM） for our register file. The proposed SNM model can be used as a guideline to predict the impact of several register parameters on the stability and optimize register file designs. To validate the proposed SNM model, we fabricated a test chip of two-write-four-read （2W4R） 1024 bits register file in a TSMC 65 nm low-power CMOS technology. The experimental result shows that the stability of our register file cells with an isolated inverter improve the conventional cells by approximately 2.4 times. Also, the supply voltage causes a fluctuation of SNM of about 65%, while temperature and transistor mismatch cause a fluctuation of SNM of about 20%.

A low standby-power fast carbon nanotube ternary SRAM cell with improved stability

Power dissipation, speed and stability are the most important parameters for multiple-valued SRAM design. To reduce the power consumption and further improve the performance of the ternary SRAM cell, we propose a low standby-power fast ternary SRAM cell based on carbon nanotube field effect transistors(CNFETs).The performance is simulated in terms of three criteria including standby-power, delay(write and read) and stability(RSNM). Compared to the novel ternary SRAM cell, our results show that the average standby-power, write and read delay of the proposed cell are reduced by 78.1%, 39.6% and 58.2%, respectively. In addition, the RSNM under process variations is 2.01× and 1.95× of the conventional and novel ternary SRAM cells, respectively.