Fluorescence probes with aggregation-induced emission(AIE)characteristics are of great importance in biomedical imaging with superior spatial and temporal resolution.However,the lack of toxicity studies and deep tissu...Fluorescence probes with aggregation-induced emission(AIE)characteristics are of great importance in biomedical imaging with superior spatial and temporal resolution.However,the lack of toxicity studies and deep tissue imaging in nonhuman primates hinders their clinical translation.Here,we report the blood chemistry and histological analysis in nonhuman primates treated with AIE probes over tenfold of an intravenous dose of clinically used indocyanine green(ICG)during a study period of 36 days to demonstrate AIE probes are nontoxic.Furthermore,through bright and nontoxic AIE probes and fluorescence imaging in the second window(NIR-II,1,000-1,700 nm),we achieve an unprecedented 1.5-centimeter-deep vascular imaging in nonhuman primates,breaking the current limitation of millimeter-deep NIR-II fluorescence imaging.Our important findings,i.e.,nontoxic features of AIE probes and centimeter-deep NIR-II vascular imaging in nonhuman primates,may facilitate successful translation of AIE probes in clinical trials.展开更多
基金This work is supported by the National Key Research and Development Program of China(Scientific and Technological Innovation Cooperation of Mainland and Macao)(2017YFE0120000)the Natural Science Foundation of China(91859117,81771906,81901812,81527901,and 31870991)+4 种基金the CAS Key Laboratory of Health Informatics(2011DP173015)the Guangdong Innovative and Entrepreneurial Research Team Program(2019ZT08Y191)the Science and Technology Innovation Fund of Shenzhen(JCYJ20170818161918918 and JCYJ20190812163614809)the Shenzhen Key Laboratory of Ultrasound Imaging and Therapy(ZDSYS201802061806314)the China Postdoctoral Science Foundation(2019M653129).
文摘Fluorescence probes with aggregation-induced emission(AIE)characteristics are of great importance in biomedical imaging with superior spatial and temporal resolution.However,the lack of toxicity studies and deep tissue imaging in nonhuman primates hinders their clinical translation.Here,we report the blood chemistry and histological analysis in nonhuman primates treated with AIE probes over tenfold of an intravenous dose of clinically used indocyanine green(ICG)during a study period of 36 days to demonstrate AIE probes are nontoxic.Furthermore,through bright and nontoxic AIE probes and fluorescence imaging in the second window(NIR-II,1,000-1,700 nm),we achieve an unprecedented 1.5-centimeter-deep vascular imaging in nonhuman primates,breaking the current limitation of millimeter-deep NIR-II fluorescence imaging.Our important findings,i.e.,nontoxic features of AIE probes and centimeter-deep NIR-II vascular imaging in nonhuman primates,may facilitate successful translation of AIE probes in clinical trials.
