We collected blood from coronavirus disease 2019(COVID-19)convalescent individuals and investigated SARS-CoV-2-specific humoral and cellular immunity in these discharged patients.Follow-up analysis in a cohort of 171 ...We collected blood from coronavirus disease 2019(COVID-19)convalescent individuals and investigated SARS-CoV-2-specific humoral and cellular immunity in these discharged patients.Follow-up analysis in a cohort of 171 patients at 4-11 months after the onset revealed high levels of IgG antibodies.A total of 78.1%(164/210)of the specimens tested positive for neutralizing antibody(NAb).SARS-CoV-2 antigen peptide pools-stimulated-IL-2 and-IFN-y response can distinguish COVID-19 convalescent individuals from healthy donors.Interestingly,NAb survival was significantly affected by the antigen peptide pools-stimulated-IL-2 response,-IL-8 response,and-IFN-y response.The antigen peptide pools-activated CD8+T cell counts were correlated with NAb.The antigen peptide pools-activated natural killer(NK)cell counts in convalescent individuals were correlated with NAb and disease severity.Our data suggested that the development of NAb is associated with the activation of T cells and NK cells.Our work provides a basis for further analysis of the protective immunity to SARS-CoV-2 and for understanding the pathogenesis of COVID-19.It also has implications for the development of an effective vaccine for SARS-CoV-2 infection.展开更多
After online publication of the article1 the authors noticed an error in the figure legends of Fig.1 and Supplementary Materials Fig.S2,where the"*P<0.05,0.05<**P<0.001,***P<0.001W.should be replaced ...After online publication of the article1 the authors noticed an error in the figure legends of Fig.1 and Supplementary Materials Fig.S2,where the"*P<0.05,0.05<**P<0.001,***P<0.001W.should be replaced by n*P<0.05,**P<0.01,***P<0.001M.The original article has been corrected.展开更多
基金This work was supported by the National Key Research and Development Program of China(2018YFE0204500)Centrally Guided Hubei Provincial Science and Technology Development Fund Project(2020ZYYD012)the Key Project for Anti-2019 Novel Coronavirus Pneumonia from the National Key Research and Development Program of China(2020YFC0845500).
文摘We collected blood from coronavirus disease 2019(COVID-19)convalescent individuals and investigated SARS-CoV-2-specific humoral and cellular immunity in these discharged patients.Follow-up analysis in a cohort of 171 patients at 4-11 months after the onset revealed high levels of IgG antibodies.A total of 78.1%(164/210)of the specimens tested positive for neutralizing antibody(NAb).SARS-CoV-2 antigen peptide pools-stimulated-IL-2 and-IFN-y response can distinguish COVID-19 convalescent individuals from healthy donors.Interestingly,NAb survival was significantly affected by the antigen peptide pools-stimulated-IL-2 response,-IL-8 response,and-IFN-y response.The antigen peptide pools-activated CD8+T cell counts were correlated with NAb.The antigen peptide pools-activated natural killer(NK)cell counts in convalescent individuals were correlated with NAb and disease severity.Our data suggested that the development of NAb is associated with the activation of T cells and NK cells.Our work provides a basis for further analysis of the protective immunity to SARS-CoV-2 and for understanding the pathogenesis of COVID-19.It also has implications for the development of an effective vaccine for SARS-CoV-2 infection.
文摘After online publication of the article1 the authors noticed an error in the figure legends of Fig.1 and Supplementary Materials Fig.S2,where the"*P<0.05,0.05<**P<0.001,***P<0.001W.should be replaced by n*P<0.05,**P<0.01,***P<0.001M.The original article has been corrected.
