MicroRNAs (miRNAs) are a functional small non-coding RNA and play essential roles in gene regulation indevelopment, differentiation and proliferation. In order to investigate the miRNAs function in Lewis rat hippocamp...MicroRNAs (miRNAs) are a functional small non-coding RNA and play essential roles in gene regulation indevelopment, differentiation and proliferation. In order to investigate the miRNAs function in Lewis rat hippocampal development, in this study, newborn and adult hippocampi were deliberately selected to analyze the miRNA expression profiles by microarray. Microarray analyses identified 22 differentially expressed miRNAs (>1.5 fold, intersection of two sets). Of these, 12 were down-regulated and 10 were up-regulated during hippocampus development. DAVID Functional Annotation Cluster (FAC) analysis of the 317 predicted target genes of down-regulated miRNAs revealed confident enrichment scores for cell adhesion and neuron development etc., indicating the functional significance and importance of these miRNAs during hippocampal development. Bioinformatic analyses of the differentially expressed miRNAs have identified a number of miRNAs with putative involvement in the hippocampus developing process. This study lays a solid foundation for further studies to clarify the important regulation function of miRNAs in brain tissue.展开更多
MicroRNAs are involved in regulation of the central nervous system (CNS) development, and miR-181d highly expressed in mature neurons. CREB is many signal pathways converged point in hippocampal neurons, and it plays ...MicroRNAs are involved in regulation of the central nervous system (CNS) development, and miR-181d highly expressed in mature neurons. CREB is many signal pathways converged point in hippocampal neurons, and it plays a crucial role in learning and memory. In this study, we detected the negative relationship between CREB1 protein and miR-181d expression in lewis rat hippocampus development. And the bioinformatic analysis showed that, CREB1 mRNA contains complementary sequence to the miR-181d seed region. Then we further demonstrated that miR-181d controls the expression level of CREB1 gene in PC12 cells by luciferase assay and western blot. Taken together, our data demonstrated that CREB1 mRNA is the target gene of miR-181d, and conformed CREB1 protein expression was regulated by miR-181d in PC12 cells.展开更多
文摘MicroRNAs (miRNAs) are a functional small non-coding RNA and play essential roles in gene regulation indevelopment, differentiation and proliferation. In order to investigate the miRNAs function in Lewis rat hippocampal development, in this study, newborn and adult hippocampi were deliberately selected to analyze the miRNA expression profiles by microarray. Microarray analyses identified 22 differentially expressed miRNAs (>1.5 fold, intersection of two sets). Of these, 12 were down-regulated and 10 were up-regulated during hippocampus development. DAVID Functional Annotation Cluster (FAC) analysis of the 317 predicted target genes of down-regulated miRNAs revealed confident enrichment scores for cell adhesion and neuron development etc., indicating the functional significance and importance of these miRNAs during hippocampal development. Bioinformatic analyses of the differentially expressed miRNAs have identified a number of miRNAs with putative involvement in the hippocampus developing process. This study lays a solid foundation for further studies to clarify the important regulation function of miRNAs in brain tissue.
文摘MicroRNAs are involved in regulation of the central nervous system (CNS) development, and miR-181d highly expressed in mature neurons. CREB is many signal pathways converged point in hippocampal neurons, and it plays a crucial role in learning and memory. In this study, we detected the negative relationship between CREB1 protein and miR-181d expression in lewis rat hippocampus development. And the bioinformatic analysis showed that, CREB1 mRNA contains complementary sequence to the miR-181d seed region. Then we further demonstrated that miR-181d controls the expression level of CREB1 gene in PC12 cells by luciferase assay and western blot. Taken together, our data demonstrated that CREB1 mRNA is the target gene of miR-181d, and conformed CREB1 protein expression was regulated by miR-181d in PC12 cells.
