Objective： To study the mechanisms in gambogic acid （GA） -induced JeKo-1 human Mantle Cell Lymphoma cell apoptosis in vitro. Methods： The proliferation of GA-treated JeKo-1 cells was measured by CCK-8 assay and Ki...Objective： To study the mechanisms in gambogic acid （GA） -induced JeKo-1 human Mantle Cell Lymphoma cell apoptosis in vitro. Methods： The proliferation of GA-treated JeKo-1 cells was measured by CCK-8 assay and Ki-67 immunocytochemical detection. Apopt0sis, cell cycle and mitochondrial membrane potential were measured by flow cytometric analysis. Caspase-3, -8 and -9 were detected by colorimetric assay. Bcl-2 and Bax were analyzed by Western blotting. Results： GA inhibited cell growth in a time- and dose- dependent manner. GA induces apoptosis in JeKo- 1 cells but not in normal bone marrow cells, which was involved in reducing the membrane potential of mitochondria, activating caspases-3, -8 and -9 and decreasing the ratio of Bd-2 and Bax without cell cycle arresting. Conclusions： GA induced apoptosis in human MCL JeKo-1 cells by regulating Bcl-2/Bax and activating caspase-3, -8 and -9 via mitochondrial pathway without affecting cell cycle.展开更多
Two large landslides successively blocked the Jinsha River at the same location in Jiangda Village on October 10 and November 3,2018,respectively.The dynamic processes and possible interactions of the two landslides n...Two large landslides successively blocked the Jinsha River at the same location in Jiangda Village on October 10 and November 3,2018,respectively.The dynamic processes and possible interactions of the two landslides need to be studied to better understand the physical processes involved,and to provide information on future disaster mitigation.We investigated their force histories and sliding directions by inverting regional broadband seismograms.The scale of the October landslide was approximately three times that of the November event.The October event revealed a particularly strong deceleration force,which may have been caused by a collision between the sliding mass and ground surface.In contrast,the November event had a relatively weaker deceleration force,indicating that it may have been gradually stopped by the landslide dam formed during the October landslide.The sliding directions of the two landslides differed significantly in terms of both horizontal and vertical directions,indicating a change in their sliding surfaces.We conclude that unconsolidated materials at the top of the October landslide continued sliding along a curved slope during the November event.From our seismic models of dynamic processes,both the October landslide and local background may have affected and even changed a subsequent landslide’s mechanism.展开更多
基金supported by a grant from the Key Project supported by medical science and technology development Foundation of Nanjing Department of Health (No. ZKX09016)
文摘Objective： To study the mechanisms in gambogic acid （GA） -induced JeKo-1 human Mantle Cell Lymphoma cell apoptosis in vitro. Methods： The proliferation of GA-treated JeKo-1 cells was measured by CCK-8 assay and Ki-67 immunocytochemical detection. Apopt0sis, cell cycle and mitochondrial membrane potential were measured by flow cytometric analysis. Caspase-3, -8 and -9 were detected by colorimetric assay. Bcl-2 and Bax were analyzed by Western blotting. Results： GA inhibited cell growth in a time- and dose- dependent manner. GA induces apoptosis in JeKo- 1 cells but not in normal bone marrow cells, which was involved in reducing the membrane potential of mitochondria, activating caspases-3, -8 and -9 and decreasing the ratio of Bd-2 and Bax without cell cycle arresting. Conclusions： GA induced apoptosis in human MCL JeKo-1 cells by regulating Bcl-2/Bax and activating caspase-3, -8 and -9 via mitochondrial pathway without affecting cell cycle.
基金This work was supported by the National Natural Science Foundation of China(Nos.42074058,41822401,and 42021003)the National Key Research and Development Program of China(No.2018YFC1503705).
文摘Two large landslides successively blocked the Jinsha River at the same location in Jiangda Village on October 10 and November 3,2018,respectively.The dynamic processes and possible interactions of the two landslides need to be studied to better understand the physical processes involved,and to provide information on future disaster mitigation.We investigated their force histories and sliding directions by inverting regional broadband seismograms.The scale of the October landslide was approximately three times that of the November event.The October event revealed a particularly strong deceleration force,which may have been caused by a collision between the sliding mass and ground surface.In contrast,the November event had a relatively weaker deceleration force,indicating that it may have been gradually stopped by the landslide dam formed during the October landslide.The sliding directions of the two landslides differed significantly in terms of both horizontal and vertical directions,indicating a change in their sliding surfaces.We conclude that unconsolidated materials at the top of the October landslide continued sliding along a curved slope during the November event.From our seismic models of dynamic processes,both the October landslide and local background may have affected and even changed a subsequent landslide’s mechanism.
