A MXene-functionalized paper-based electrochemical immunosensor for label-free detection of cardiac troponin I

Convenient,rapid,and accurate detection of cardiac troponin I(cTnI)is crucial in early diagnosis of acute myocardial infarction(AMI).A paper-based electrochemical immunosensor is a promising choice in this field,because of the flexibility,porosity,and cost-efficacy of the paper.However,paper is poor in electronic conductivity and surface functionality.Herein,we report a paper-based electrochemical immunosensor for the label-free detection of cTnI with the working electrode modified by MXene(Ti_(3)C_(2))nanosheets.In order to immobilize the bio-receptor(anti-cTnI)on the MXene-modified working electrode,the MXene nanosheets were functionalized by aminosilane,and the functionalized MXene was immobilized onto the surface of the working electrode through Nafion.The large surface area of the MXene nanosheets facilitates the immobilization of antibodies,and the excellent conductivity facilitates the electron transfer between the electrochemical species and the underlying electrode surface.As a result,the paper-based immunosensor could detect cTnI within a wide range of 5-100 ng/mL with a detection limit of 0.58 ng/mL.The immunosensor also shows outstanding selectivity and good repeatability.Our MXene-modified paper-based electrochemical immunosensor enables fast and sensitive detection of cTnI,which may be used in real-time and cost-efficient monitoring of AMI diseases in clinics.

Flat-plate hypersonic boundary-layer ?ow instability and transition prediction considering air dissociation

The effects of air dissociation on ?at-plate hypersonic boundary-layer ?ow instability and transition prediction are studied. The air dissociation reactions are assumed to be in the chemical equilibrium. Based on the ?at-plate boundary layer, the ?ow stability is analyzed for the Mach numbers from 8 to 15. The results reveal that the consideration of air dissociation leads to a decrease in the unstable region of the ?rst-mode wave and an increase in the maximum growth rate of the second mode. High frequencies appear earlier in the third mode than in the perfect gas model, and the unstable region moves to a lower frequency region. When the Mach number increases, the second-mode wave dominates the transition process, and the third-mode wave has little effect on the transition. Moreover, when the Mach number increases from 8 to 12, the N-factor envelope becomes higher, and the transition is promoted. However, when the Mach number exceeds 12, the N-factor envelope becomes lower, and the transition is delayed. The N-factor envelope decreases gradually with the increase in the altitude or Mach number.

Improvement for expansion of parabolized stability equation method in boundary layer stability analysis

An improved expansion of the parabolized stability equation(iEPSE) method is proposed for the accurate linear instability prediction in boundary layers. It is a local eigenvalue problem, and the streamwise wavenumber α and its streamwise gradient dα/dx are unknown variables. This eigenvalue problem is solved for the eigenvalue dα/dx with an initial α, and the correction of α is performed with the conservation relation used in the PSE. The i EPSE is validated in several compressible and incompressible boundary layers. The computational results show that the prediction accuracy of the i EPSE is significantly higher than that of the ESPE, and it is in excellent agreement with the PSE which is regarded as the baseline for comparison. In addition, the unphysical multiple eigenmode problem in the EPSE is solved by using the i EPSE. As a local non-parallel stability analysis tool, the i EPSE has great potential application in the eNtransition prediction in general three-dimensional boundary layers.

Effects of Light Quality on Carbon and Nitrogen Assimilation and Root Activity of Eggplant

[Objective] This study was conducted to provide theoretical and data reference for high-quality high-yield production of Shangqie 1 under controlled light quality environment and light quality control of other eggplant varieties. [Method] With Shangqie1 as an experiment material, the effects of red light, blue light, red-blue light, red-blue light and white light(control) on the carbon-nitrogen metabolism and root vitality of eggplant were investigated. [Result] Different light quality significantly affected sugar and starch contents in leaves of eggplant. Under the red-blue light(5∶1) treatment, the sucrose, total sugar and starch contents were the highest, and the values were higher than those under the white light treatment by 3.47%, 13.61% and 33.49%,respectively. The fructose content was the highest under white light. Under red-blue light(3∶1) and red-blue light(5∶1), the nitrate nitrogen contents were higher than that under white light, indicating that compound light affects nitrate content not only by simple superposition of light quality, but also by other type of interaction effect. The free amino acid and soluble protein contents under blue, red-blue(3∶1), red-blue(5∶1) and red light treatments were all higher than those under the white light treatment, and the blue light treatment exhibited the highest values, which were higher than those under white light by 24.89% and 46.62%, respectively, with significant differences. The red-blue light(5 ∶1) treatment exhibited the highest root vitality of eggplant, which was higher than that under the white light treatment by 31.85%. The red light treatment had the second highest root vitality, which was higher than that under the white light treatment by 19.30%, followed by the red-blue light(5∶1) treatment, and the blue light treatment showed the lowest value. [Conclusion] Under red-blue light(5 ∶1) treatment, eggplant had vigorous carbon-nitrogen metabolism and the highest root vitality.