Clean and efficient recycling of spent lithium-ion batteries(LIBs)has become an urgent need to promote sustainable and rapid development of human society.Therefore,we provide a critical and comprehensive overview of th...Clean and efficient recycling of spent lithium-ion batteries(LIBs)has become an urgent need to promote sustainable and rapid development of human society.Therefore,we provide a critical and comprehensive overview of the various technologies for recycling spent LIBs,starting with lithium-ion power batteries.Recent research on raw material collection,metallurgical recovery,separation and purification is highlighted,particularly in terms of all aspects of economic efficiency,energy consumption,technology transformation and policy management.Mechanisms and pathways for transformative full-component recovery of spent LIBs are explored,revealing a clean and efficient closed-loop recovery mechanism.Optimization methods are proposed for future recycling technologies,with a focus on how future research directions can be industrialized.Ultimately,based on life-cycle assessment,the challenges of future recycling are revealed from the LIBs supply chain and stability of the supply chain of the new energy battery industry to provide an outlook on clean and efficient short process recycling technologies.This work is designed to support the sustainable development of the new energy power industry,to help meet the needs of global decarbonization strategies and to respond to the major needs of industrialized recycling.展开更多
This study focuses on the analysis of the Chinese composition writing performance of fourth,fifth,and sixth grade students in 16 selected schools in Longhua District,Shenzhen during the spring semester of 2023.Using L...This study focuses on the analysis of the Chinese composition writing performance of fourth,fifth,and sixth grade students in 16 selected schools in Longhua District,Shenzhen during the spring semester of 2023.Using LIWC(Linguistic Inquiry and Word Count)as a text analysis tool,the study explores the impact of LIWC categories on writing performance which is scaled by score.The results show that the simple LIWC word categories have a significant positive influence on the composition scores of lower-grade students;while complex LIWC word categories have a significant negative influence on the composition scores of lower-grade students but a significant positive influence on the composition scores of higher-grade students.Process word categories have a positive influence on the composition scores of all three grades,but the impact of complex process word categories increases as the grade level rises.展开更多
Resulting from the development of electric vehicles,high energy-density Li-S batteries have recently attracted ever-increasing attentions worldwide.However,continuous dissolution of cathodic sulfur and followed shuttl...Resulting from the development of electric vehicles,high energy-density Li-S batteries have recently attracted ever-increasing attentions worldwide.However,continuous dissolution of cathodic sulfur and followed shuttle effect of polysulfides lead to very limited service lifetime for currently-applied Li-S batteries.Herein,a 3 D porous graphene aerogel(GA)decorated with high exposure of anatase TiO2(001)nanoplatelets is proposed as robust host to immobilize cathodic sulfur.Compared with commonly used TiO2(101)nanoparticles,the Ti O2(001)nanoplatelets have highly matched lattices with graphene(002)nanosheets,thus facilitating the electronic transfer.The in-site assembled TiO2@GA host exhibits superior sulfur-immobilized capability,which cannot only entrap sulfur by physical confinement,but also capture dissoluble sulfurous species by chemical bonding.The fabricated S@TiO2@GA cathode shows excellent electrochemical performance with high discharge capacity,superior rate capability,and durable cycling stability as well,supposed to be a promising cathode for high-performance Li-S battery applications.展开更多
Plant height is one of the most important agronomic traits that directly determines plant architecture,and compact or dwarf plants can allow for increased planting density and land utilization as well as increased lod...Plant height is one of the most important agronomic traits that directly determines plant architecture,and compact or dwarf plants can allow for increased planting density and land utilization as well as increased lodging resistance and economic yield.At least four dwarf/semidwarf genes have been identified in different melon varieties,but none of them have been cloned,and little is known about the molecular mechanisms underlying internode elongation in melon.Here,we report map-based cloning and functional characterization of the first semidwarf gene short internode(Cmsi)in melon,which encodes an ERECTA-like receptor kinase regulating internode elongation.Spatial-temporal expression analyses revealed that CmSI exhibited high expression in the vascular bundle of the main stem during internode elongation.The expression level of CmSI was positively correlated with stem length in the different melon varieties examined.Ectopic expression of CmSI in Arabidopsis and cucumber suggested CmSI as a positive regulator of internode elongation in both species.Phytohormone quantitation and transcriptome analysis showed that the auxin content and the expression levels of a number of genes involved in the auxin signaling pathway were altered in the semidwarf mutant,including several well-known auxin transporters,such as members of the ABCB family and PINFORMED genes.A melon polar auxin transport protein CmPIN2 was identified by protein–protein interaction assay as physically interacting with CmSI to modulate auxin signaling.Thus,CmSI functions in an auxin-dependent regulatory pathway to control internode elongation in melon.Our findings revealed that the ERECTA family gene CmSI regulates stem elongation in melon through auxin signaling,which can directly affect polar auxin transport.展开更多
Taking the rural left-behind elderly as the research object,this paper made an in-depth analysis of the living needs of the rural"empty-nest elderly".Combined with the current market development situation of...Taking the rural left-behind elderly as the research object,this paper made an in-depth analysis of the living needs of the rural"empty-nest elderly".Combined with the current market development situation of the elderly-friendly bathroom sliding door in China,it proposed the design scheme and industrial development strategy of the suspended bathroom sliding door,to provide a certain reference for the elderly-friendly development of home decoration basic materials for elderly homes in rural areas.展开更多
Citrus sinensis is the most cultivated and economically valuable Citrus species in the world,whose genome has been assembled by three generation sequencings.However,chromosome recognition remains a problem due to the ...Citrus sinensis is the most cultivated and economically valuable Citrus species in the world,whose genome has been assembled by three generation sequencings.However,chromosome recognition remains a problem due to the small size of chromosomes,and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome.Here,we employ fluorescence in situ hybridization(FISH)with 9 chromosome painting probes,30 oligo pools,and 8 repetitive sequences to visualize 18 chromosomes.Then,we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3(CMA)staining.By this approach,we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions,which enables us to visualize significant differences between homologous chromosomes.Based on the map,we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site.In particular,we discover the translocation regions of chromosomes 4 and 9 in C.sinensis“Valencia.”The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus.展开更多
Deep learning has been widely used for plant disease recognition in smart agriculture and has proven to be a powerful tool for image classification and pattern recognition.However,it has limited interpretability for d...Deep learning has been widely used for plant disease recognition in smart agriculture and has proven to be a powerful tool for image classification and pattern recognition.However,it has limited interpretability for deep features.With the transfer of expert knowledge,handcrafted features provide a new way for personalized diagnosis of plant diseases.However,irrelevant and redundant features lead to high dimensionality.In this study,we proposed a swarm intelligence algorithm for feature selection[salp swarm algorithm for feature selection(SSAFS)]in image-based plant disease detection.SSAFS is employed to determine the ideal combination of handcrafted features to maximize classification success while minimizing the number of features.To verify the effectiveness of the developed SSAFS algorithm,we conducted experimental studies using SSAFS and 5 metaheuristic algorithms.Several evaluation metrics were used to evaluate and analyze the performance of these methods on 4 datasets from the UCI machine learning repository and 6 plant phenomics datasets from PlantVillage.Experimental results and statistical analyses validated the outstanding performance of SSAFS compared to existing state-of-the-art algorithms,confirming the superiority of SSAFS in exploring the feature space and identifying the most valuable features for diseased plant image classification.This computational tool will allow us to explore an optimal combination of handcrafted features to improve plant disease recognition accuracy and processing time.展开更多
Dear Editor Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease of wheat throughout the world. One of the most important environmental-friendly and economical methods to ...Dear Editor Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease of wheat throughout the world. One of the most important environmental-friendly and economical methods to reduce wheat loss caused by Bgt is to develop highly resistant varieties (Kuraparthy et al., 2007). Pm21 from the wild species Haynaldia villosa (also known as Dasypyrum villosum) confers high resistance to Bgt in wheat throughout all growth stages. It has now become one of the most highly effective genetic loci introgressed into wheat from wild species, and the commercial varieties harboring Pm21 have been widely used in wheat production with more than 4 million hectares in China.展开更多
Environmental friendly recycling process for Nd-Fe-B sintered magnet sludges generated in the manufacturing process, which contain large amount of rare earth, including Nd, Pr and Dy, is badly needed so far. In presen...Environmental friendly recycling process for Nd-Fe-B sintered magnet sludges generated in the manufacturing process, which contain large amount of rare earth, including Nd, Pr and Dy, is badly needed so far. In present study, we have developed an effective route to obtain recycled sintered magnets from Nd-Fe-B sintered magnet sludges by calcium reduction-diffusion(RD) process. Compared to conventional recycling process, our research is focused on recovering most of the useful elements, including Nd, Pr, Dy, Co, and Fe together instead of just rare earth elements. To improve the recycling efficiency and reduce pollution, the co-precipitating parameters were simulated and calculated using MATLAB software. Most of useful elements were recovered by a co-precipitation method, and the obtained composite powders were then directly fabricated as recycled Nd-Fe-B powders by a calcium reduction-diffusion(RD) method. The recovery rates are 98%, 99%, 99%, 93%, and 99%, for Nd, Pr, Dy, Co, and Fe, respectively. The amount of useful elements contained in the recovered composite powders is greater than99.71 wt%. The process of RD for synthesizing NdFeB and subsequently removing CaO was thoroughly investigated. Furthermore, the recycled Nd-Fe-B magnet exhibits a remanence of 1.1 T, a coercivity of1053 kA/m, and an energy product of 235.6 kJ/m~3, respectively, indicating that recycled Nd-Fe-B sintered magnet was successfully recovered from the severely contaminated sludges via an effective recycling route.展开更多
Precursor mRNA(pre-mRNA)splicing is essential for gene expression in most eukaryotic organisms.Previous studies from mammals,Drosophila,and yeast show that the majority of splicing events occurs co-transcriptionally.I...Precursor mRNA(pre-mRNA)splicing is essential for gene expression in most eukaryotic organisms.Previous studies from mammals,Drosophila,and yeast show that the majority of splicing events occurs co-transcriptionally.In plants,however,the features of co-transcriptional splicing(CTS)and its regulation still remain largely unknown.Here,we used chromatin-bound RNA sequencing to study CTS in Arabidopsis thaliana.We found that CTS is widespread in Arabidopsis seedlings,with a large proportion of alternative splicing events determined co-transcriptionally.CTS efficiency correlated with gene expression level,the chromatin landscape and,most surprisingly,the number of introns and exons of individual genes,but is independent of gene length.In combination with enhanced crosslinking and immunoprecipitation sequencing analysis,we further showed that the hnRNP-like proteins RZ-1B and RZ-1C promote efficient CTS globally through direct binding,frequently to exonic sequences.Notably,this general effect of RZ-1B/1C on splicing promotion is mainly observed at the chromatin level,not at the mRNA level.RZ-1C promotes CTS of multiple-exon genes in association with its binding to regions both proximal and distal to the regulated introns.We propose that RZ-1C promotes efficient CTS of genes with multiple exons through cooperative interactions with many exons,introns,and splicing factors.Our work thus reveals important features of CTS in plants and provides methodologies for the investigation of CTS and RNA-binding proteins in plants.展开更多
New-generation human body motion sensors for wearable electronics and intelligent medicine are required to comply with stringent requirements in terms of ultralight weight,flexibility,stability,biocompatibility,and ex...New-generation human body motion sensors for wearable electronics and intelligent medicine are required to comply with stringent requirements in terms of ultralight weight,flexibility,stability,biocompatibility,and extreme precision.However,conventional sensors are hard to fulfill all these criteria due to their rigid structure,high-density sensing materials used as the constituents,as well as hermetical and compact assembly strategy.Here,we report an ultralight sensing material based on radial anisotropic porous silver fiber(RAPSF),which has been manufactured by phase separation and temperature-controlled grain growth strategy on a modified blow-spinning system.The resistance of RAPSF could be dynamically adjusted depending on the deflected shape.Furthermore,an all-fiber motion sensor(AFMS)with an ultra-low density of 68.70 mg cm^(−3) and an overall weigh of 7.95 mg was fabricated via layer-by-layer assembly.The sensor exhibited outstanding flexibility,breathability,biocompatibility,and remarkable body motion recognition ability.Moreover,the AFMS was shown to have great potential as an artificial intelligence throat sensor for throat state identification at the accuracy above 85%,allowing one to spot the early onset of the viral throat illness.展开更多
To the Editor:The answer to the"Riddle of Sphinx"is"human",whose image is both negative and positive.It is often used as a metaphor for complex,mysterious,and incomprehensible problems.With the det...To the Editor:The answer to the"Riddle of Sphinx"is"human",whose image is both negative and positive.It is often used as a metaphor for complex,mysterious,and incomprehensible problems.With the deterioration of modern living environment and the aggravation of stress,the incidence of the tumor is on the rise.Excessive or prolonged stress can lead to acute or chronic organ dysfunction and metabolic disorders.展开更多
基金supported by the National Key R&D Program of China,China(2022YFC3902600)CAS Project for Young Scientists in Basic Research,China(YSBR-044)+1 种基金Guangdong Basic and Applied Basic Research Foundation,China(2021B1515020068)China Postdoctoral Science Foundation,China(2023M733510).
文摘Clean and efficient recycling of spent lithium-ion batteries(LIBs)has become an urgent need to promote sustainable and rapid development of human society.Therefore,we provide a critical and comprehensive overview of the various technologies for recycling spent LIBs,starting with lithium-ion power batteries.Recent research on raw material collection,metallurgical recovery,separation and purification is highlighted,particularly in terms of all aspects of economic efficiency,energy consumption,technology transformation and policy management.Mechanisms and pathways for transformative full-component recovery of spent LIBs are explored,revealing a clean and efficient closed-loop recovery mechanism.Optimization methods are proposed for future recycling technologies,with a focus on how future research directions can be industrialized.Ultimately,based on life-cycle assessment,the challenges of future recycling are revealed from the LIBs supply chain and stability of the supply chain of the new energy battery industry to provide an outlook on clean and efficient short process recycling technologies.This work is designed to support the sustainable development of the new energy power industry,to help meet the needs of global decarbonization strategies and to respond to the major needs of industrialized recycling.
文摘This study focuses on the analysis of the Chinese composition writing performance of fourth,fifth,and sixth grade students in 16 selected schools in Longhua District,Shenzhen during the spring semester of 2023.Using LIWC(Linguistic Inquiry and Word Count)as a text analysis tool,the study explores the impact of LIWC categories on writing performance which is scaled by score.The results show that the simple LIWC word categories have a significant positive influence on the composition scores of lower-grade students;while complex LIWC word categories have a significant negative influence on the composition scores of lower-grade students but a significant positive influence on the composition scores of higher-grade students.Process word categories have a positive influence on the composition scores of all three grades,but the impact of complex process word categories increases as the grade level rises.
基金financially supported by the National Key R and D Program of China(No.2019YFA0210300)the Hunan Provincial Natural Science Foundation of China(No.2019JJ40359)+1 种基金the Hunan Provincial S and T Plan of China(Nos.2017TP1001,2016TP1007)the Open-End Fund for the Valuable and Precision Instruments of Central South University(CSUZC2020016)。
文摘Resulting from the development of electric vehicles,high energy-density Li-S batteries have recently attracted ever-increasing attentions worldwide.However,continuous dissolution of cathodic sulfur and followed shuttle effect of polysulfides lead to very limited service lifetime for currently-applied Li-S batteries.Herein,a 3 D porous graphene aerogel(GA)decorated with high exposure of anatase TiO2(001)nanoplatelets is proposed as robust host to immobilize cathodic sulfur.Compared with commonly used TiO2(101)nanoparticles,the Ti O2(001)nanoplatelets have highly matched lattices with graphene(002)nanosheets,thus facilitating the electronic transfer.The in-site assembled TiO2@GA host exhibits superior sulfur-immobilized capability,which cannot only entrap sulfur by physical confinement,but also capture dissoluble sulfurous species by chemical bonding.The fabricated S@TiO2@GA cathode shows excellent electrochemical performance with high discharge capacity,superior rate capability,and durable cycling stability as well,supposed to be a promising cathode for high-performance Li-S battery applications.
基金supported by grants from the National Natural Science Foundation of China(31872133)the Project for Scientific and Technological Activities of Overseas Students of Henan Province,the Zhongyuan Youth Talent Support Program(ZYQR201912161)the Program for Science&Technology Innovation Talents in Universities of Henan Province(20HASTIT035).
文摘Plant height is one of the most important agronomic traits that directly determines plant architecture,and compact or dwarf plants can allow for increased planting density and land utilization as well as increased lodging resistance and economic yield.At least four dwarf/semidwarf genes have been identified in different melon varieties,but none of them have been cloned,and little is known about the molecular mechanisms underlying internode elongation in melon.Here,we report map-based cloning and functional characterization of the first semidwarf gene short internode(Cmsi)in melon,which encodes an ERECTA-like receptor kinase regulating internode elongation.Spatial-temporal expression analyses revealed that CmSI exhibited high expression in the vascular bundle of the main stem during internode elongation.The expression level of CmSI was positively correlated with stem length in the different melon varieties examined.Ectopic expression of CmSI in Arabidopsis and cucumber suggested CmSI as a positive regulator of internode elongation in both species.Phytohormone quantitation and transcriptome analysis showed that the auxin content and the expression levels of a number of genes involved in the auxin signaling pathway were altered in the semidwarf mutant,including several well-known auxin transporters,such as members of the ABCB family and PINFORMED genes.A melon polar auxin transport protein CmPIN2 was identified by protein–protein interaction assay as physically interacting with CmSI to modulate auxin signaling.Thus,CmSI functions in an auxin-dependent regulatory pathway to control internode elongation in melon.Our findings revealed that the ERECTA family gene CmSI regulates stem elongation in melon through auxin signaling,which can directly affect polar auxin transport.
基金Supported by National Innovation and Entrepreneurship Training Program for College Students in 2021 (202110414018)
文摘Taking the rural left-behind elderly as the research object,this paper made an in-depth analysis of the living needs of the rural"empty-nest elderly".Combined with the current market development situation of the elderly-friendly bathroom sliding door in China,it proposed the design scheme and industrial development strategy of the suspended bathroom sliding door,to provide a certain reference for the elderly-friendly development of home decoration basic materials for elderly homes in rural areas.
基金supported by the National Natural Science Foundation of China(31970525,31672112)the National Key Research and Development Project of China(2019YFD1001401-GJ03)+4 种基金Advanced Foreign Experts Project(G2021157012L,G20200017071)Cultivating Fund Project of Hubei Hongshan Laboratory(2022hspy002)Fundamental Research Funds for the Central Universities(2662018PY099)from Chinese governmentBreeding of breakthrough new citrus varieties and creation of new germplasms,Science and Technology Planning Project of Sichuan Province(2021YFYZ0023-02)Comprehensive experimental station of middle and late maturing citrus in Chengdu,China Agriculture Research System(CARS-26)。
文摘Citrus sinensis is the most cultivated and economically valuable Citrus species in the world,whose genome has been assembled by three generation sequencings.However,chromosome recognition remains a problem due to the small size of chromosomes,and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome.Here,we employ fluorescence in situ hybridization(FISH)with 9 chromosome painting probes,30 oligo pools,and 8 repetitive sequences to visualize 18 chromosomes.Then,we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3(CMA)staining.By this approach,we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions,which enables us to visualize significant differences between homologous chromosomes.Based on the map,we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site.In particular,we discover the translocation regions of chromosomes 4 and 9 in C.sinensis“Valencia.”The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus.
基金supported by the Nat-ural Science Foundation of Jiangsu Province(No.BK20211210)the Fundamental Research Funds for the Central Universities(KYCXJC2022005)+1 种基金the startup award of new professors at Nanjing Agricultural University(No.106/804001)supported by the Natural Science Foundation of Zhejiang province(No.LY20F020003).
文摘Deep learning has been widely used for plant disease recognition in smart agriculture and has proven to be a powerful tool for image classification and pattern recognition.However,it has limited interpretability for deep features.With the transfer of expert knowledge,handcrafted features provide a new way for personalized diagnosis of plant diseases.However,irrelevant and redundant features lead to high dimensionality.In this study,we proposed a swarm intelligence algorithm for feature selection[salp swarm algorithm for feature selection(SSAFS)]in image-based plant disease detection.SSAFS is employed to determine the ideal combination of handcrafted features to maximize classification success while minimizing the number of features.To verify the effectiveness of the developed SSAFS algorithm,we conducted experimental studies using SSAFS and 5 metaheuristic algorithms.Several evaluation metrics were used to evaluate and analyze the performance of these methods on 4 datasets from the UCI machine learning repository and 6 plant phenomics datasets from PlantVillage.Experimental results and statistical analyses validated the outstanding performance of SSAFS compared to existing state-of-the-art algorithms,confirming the superiority of SSAFS in exploring the feature space and identifying the most valuable features for diseased plant image classification.This computational tool will allow us to explore an optimal combination of handcrafted features to improve plant disease recognition accuracy and processing time.
基金This work was supported by the Important National Science and Technology Specific Projects of Transgenic Research (Grant No. 2018ZX0800963B), the Natural Science Foundation of China (Grant No. 31671685, 31771779), the Fundamental Research Funds for the National Central Universities (Grant No. KYZ201601), the Czech Science Foundation (Grant No. P501/12/G090), and the Ministry of Education, Youth and Sports of the Czech Republic (Grant No. LO1204 from the National Program of Sustainability I).
文摘Dear Editor Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive disease of wheat throughout the world. One of the most important environmental-friendly and economical methods to reduce wheat loss caused by Bgt is to develop highly resistant varieties (Kuraparthy et al., 2007). Pm21 from the wild species Haynaldia villosa (also known as Dasypyrum villosum) confers high resistance to Bgt in wheat throughout all growth stages. It has now become one of the most highly effective genetic loci introgressed into wheat from wild species, and the commercial varieties harboring Pm21 have been widely used in wheat production with more than 4 million hectares in China.
基金Project supported by the Beijing Municipal Natural Science Foundation(2172012)the International S&T Cooperation Program of China(2015DFG52020)the National High Technology Research and Development Program of China(2012AA063201)
文摘Environmental friendly recycling process for Nd-Fe-B sintered magnet sludges generated in the manufacturing process, which contain large amount of rare earth, including Nd, Pr and Dy, is badly needed so far. In present study, we have developed an effective route to obtain recycled sintered magnets from Nd-Fe-B sintered magnet sludges by calcium reduction-diffusion(RD) process. Compared to conventional recycling process, our research is focused on recovering most of the useful elements, including Nd, Pr, Dy, Co, and Fe together instead of just rare earth elements. To improve the recycling efficiency and reduce pollution, the co-precipitating parameters were simulated and calculated using MATLAB software. Most of useful elements were recovered by a co-precipitation method, and the obtained composite powders were then directly fabricated as recycled Nd-Fe-B powders by a calcium reduction-diffusion(RD) method. The recovery rates are 98%, 99%, 99%, 93%, and 99%, for Nd, Pr, Dy, Co, and Fe, respectively. The amount of useful elements contained in the recovered composite powders is greater than99.71 wt%. The process of RD for synthesizing NdFeB and subsequently removing CaO was thoroughly investigated. Furthermore, the recycled Nd-Fe-B magnet exhibits a remanence of 1.1 T, a coercivity of1053 kA/m, and an energy product of 235.6 kJ/m~3, respectively, indicating that recycled Nd-Fe-B sintered magnet was successfully recovered from the severely contaminated sludges via an effective recycling route.
基金supported by Guangdong Innovative and Entrepreneurial Research Team Program(2016ZT06S172)the Shenzhen Sci-Tech Fund No.KYTDPT20181011104005the National Natural Science Foundation of China(31771365 to Z.W.and 31800268 to D.Z.).
文摘Precursor mRNA(pre-mRNA)splicing is essential for gene expression in most eukaryotic organisms.Previous studies from mammals,Drosophila,and yeast show that the majority of splicing events occurs co-transcriptionally.In plants,however,the features of co-transcriptional splicing(CTS)and its regulation still remain largely unknown.Here,we used chromatin-bound RNA sequencing to study CTS in Arabidopsis thaliana.We found that CTS is widespread in Arabidopsis seedlings,with a large proportion of alternative splicing events determined co-transcriptionally.CTS efficiency correlated with gene expression level,the chromatin landscape and,most surprisingly,the number of introns and exons of individual genes,but is independent of gene length.In combination with enhanced crosslinking and immunoprecipitation sequencing analysis,we further showed that the hnRNP-like proteins RZ-1B and RZ-1C promote efficient CTS globally through direct binding,frequently to exonic sequences.Notably,this general effect of RZ-1B/1C on splicing promotion is mainly observed at the chromatin level,not at the mRNA level.RZ-1C promotes CTS of multiple-exon genes in association with its binding to regions both proximal and distal to the regulated introns.We propose that RZ-1C promotes efficient CTS of genes with multiple exons through cooperative interactions with many exons,introns,and splicing factors.Our work thus reveals important features of CTS in plants and provides methodologies for the investigation of CTS and RNA-binding proteins in plants.
基金supported by the Fundamental Research Funds for the Central Universities (2021XD-A04-1)National Natural Science Foundations of China (Grant 62104051,51661135025,51706117 and U1564205)National Basic Research of China (Grants 2018YFB0104404).
文摘New-generation human body motion sensors for wearable electronics and intelligent medicine are required to comply with stringent requirements in terms of ultralight weight,flexibility,stability,biocompatibility,and extreme precision.However,conventional sensors are hard to fulfill all these criteria due to their rigid structure,high-density sensing materials used as the constituents,as well as hermetical and compact assembly strategy.Here,we report an ultralight sensing material based on radial anisotropic porous silver fiber(RAPSF),which has been manufactured by phase separation and temperature-controlled grain growth strategy on a modified blow-spinning system.The resistance of RAPSF could be dynamically adjusted depending on the deflected shape.Furthermore,an all-fiber motion sensor(AFMS)with an ultra-low density of 68.70 mg cm^(−3) and an overall weigh of 7.95 mg was fabricated via layer-by-layer assembly.The sensor exhibited outstanding flexibility,breathability,biocompatibility,and remarkable body motion recognition ability.Moreover,the AFMS was shown to have great potential as an artificial intelligence throat sensor for throat state identification at the accuracy above 85%,allowing one to spot the early onset of the viral throat illness.
文摘To the Editor:The answer to the"Riddle of Sphinx"is"human",whose image is both negative and positive.It is often used as a metaphor for complex,mysterious,and incomprehensible problems.With the deterioration of modern living environment and the aggravation of stress,the incidence of the tumor is on the rise.Excessive or prolonged stress can lead to acute or chronic organ dysfunction and metabolic disorders.