Advanced brain organoids provide promising platforms for deciphering the cellular and molecular processes of human neural development and diseases.Although various studies and reviews have described developments and a...Advanced brain organoids provide promising platforms for deciphering the cellular and molecular processes of human neural development and diseases.Although various studies and reviews have described developments and advancements in brain organoids,few studies have comprehensively summarized and analyzed the global trends in this area of neuroscience.To identify and further facilitate the development of cerebral organoids,we utilized bibliometrics and visualization methods to analyze the global trends and evolution of brain organoids in the last 10 years.First,annual publications,countries/regions,organizations,journals,authors,co-citations,and keywords relating to brain organoids were identified.The hotspots in this field were also systematically identified.Subsequently,current applications for brain organoids in neuroscience,including human neural development,neural disorders,infectious diseases,regenerative medicine,drug discovery,and toxicity assessment studies,are comprehensively discussed.Towards that end,several considerations regarding the current challenges in brain organoid research and future strategies to advance neuroscience will be presented to further promote their application in neurological research.展开更多
Prunella vulgaris,a traditional Chinese medicine(TCM),exerts a significant hypotensive effect,particularly in managing various forms of hypertension.Nonetheless,the precise antihypertensive constituents and their resp...Prunella vulgaris,a traditional Chinese medicine(TCM),exerts a significant hypotensive effect,particularly in managing various forms of hypertension.Nonetheless,the precise antihypertensive constituents and their respective targets remain elusive.This study endeavored to identify the hypotensive components of P.vulgaris and elucidated its mode of action based on hypotensive targets.Utilizing the Systematic Pharmacology of Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),UniProt,GeneCards,STRING,RCSB,and the Human Genome Annotation and Analysis Database(Metascape),we conducted a screening of active ingredients and hypotensive targets.The Network Analyzer software Cytoscape 3.9.0 facilitated a comprehensive analysis of the active ingredients.Molecular docking was executed employing Sybyl-X 2.0 and Discovery Studio 2019.Predictions and analyses of the pharmacokinetics and toxicity of active ingredients were performed using the ADMETlab 2.0 online platform.Eight active compounds(1–8)and 11 hypotensive targets were identified,with IL1B and PPARG exhibiting a high degree of correlation.Dominant biological processes included negative regulation of apoptotic processes,positive regulation of gene expression,response to xenobiotic stimulus,and response to hypoxia.KEGG analysis unveiled core pharmacological mechanisms,notably fluid shear stress and atherosclerosis,lipid and atherosclerosis,P13K-Akt,MAPK,and relaxin signaling pathways.Compounds 5–8 demonstrated robust interactions with multiple targets through hydrogen bonds,van der Waals forces,and pi-alkyl interactions,which serve as primary stabilizers of docking complexes.Notably,compound 7 exhibited promising ADMET prediction results,suggesting its potential for drug molecule development.Our findings underscored the synergistic effects of P.vulgaris on multiple targets and pathways in hypertension treatment,reflecting the characteristic multi-component and multi-target effects of TCM.展开更多
基金supported by the National Natural Science Foundation of China,Nos.82204083(to ML)and 12372303(to BW)the Natural Science Foundation of Chongqing,No.cstc2021jcy-jmsxmX0171(to ML).
文摘Advanced brain organoids provide promising platforms for deciphering the cellular and molecular processes of human neural development and diseases.Although various studies and reviews have described developments and advancements in brain organoids,few studies have comprehensively summarized and analyzed the global trends in this area of neuroscience.To identify and further facilitate the development of cerebral organoids,we utilized bibliometrics and visualization methods to analyze the global trends and evolution of brain organoids in the last 10 years.First,annual publications,countries/regions,organizations,journals,authors,co-citations,and keywords relating to brain organoids were identified.The hotspots in this field were also systematically identified.Subsequently,current applications for brain organoids in neuroscience,including human neural development,neural disorders,infectious diseases,regenerative medicine,drug discovery,and toxicity assessment studies,are comprehensively discussed.Towards that end,several considerations regarding the current challenges in brain organoid research and future strategies to advance neuroscience will be presented to further promote their application in neurological research.
基金National Natural Science Foundation of China(Grant No.21702178)Project of Science and Technology Department of Henan Province(Grant No.242102310503)+6 种基金Postgraduate Education Reform and Quality(Grant No.YJS2023AL082)Graduate Education Reform Project of Henan Province(Grant No.2023SJGLX294Y)the Training Plan of Young Backbone Teachers in Universities of Henan Province(Grant No.2021GGJS144)the Key Scientific Research Program in Universities of Henan Province(Grant No.23A350012)the National Undergraduate Training Program for Innovation and Entrepreneurship(Grant No.202310480020 and 202410480007)School-land Cooperation Project(Grant No.2023HX181)Training Plan of Young Backbone Teachers in Secondary College of Xuchang University(2023)。
基金National Natural Science Foundation of China(Grant No.21702178)Training Plan of Young Backbone Teachers in Universities of Henan Province(Grant No.2021GGJS144)+5 种基金Key Scientific Research Program in Universities of Henan Province(Grant No.22A350009 and 23A350012)National Undergraduate Training Program for Innovation and Entrepreneurship(Grant No.202410480007 and 202310480020)Undergraduate Training Program for Innovation and Entrepreneurship of Henan Province(Grant No.202210480020 and 202210480021)Scientific Research Innovation Team of Xuchang University(Grant No.2022CXTD007)Horizontal Cooperation Project(Grant No.2023HX181)Undergraduate Training Program for Innovation and Entrepreneurship of Xuchang University(Grant No.X202210480016)。
文摘Prunella vulgaris,a traditional Chinese medicine(TCM),exerts a significant hypotensive effect,particularly in managing various forms of hypertension.Nonetheless,the precise antihypertensive constituents and their respective targets remain elusive.This study endeavored to identify the hypotensive components of P.vulgaris and elucidated its mode of action based on hypotensive targets.Utilizing the Systematic Pharmacology of Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),UniProt,GeneCards,STRING,RCSB,and the Human Genome Annotation and Analysis Database(Metascape),we conducted a screening of active ingredients and hypotensive targets.The Network Analyzer software Cytoscape 3.9.0 facilitated a comprehensive analysis of the active ingredients.Molecular docking was executed employing Sybyl-X 2.0 and Discovery Studio 2019.Predictions and analyses of the pharmacokinetics and toxicity of active ingredients were performed using the ADMETlab 2.0 online platform.Eight active compounds(1–8)and 11 hypotensive targets were identified,with IL1B and PPARG exhibiting a high degree of correlation.Dominant biological processes included negative regulation of apoptotic processes,positive regulation of gene expression,response to xenobiotic stimulus,and response to hypoxia.KEGG analysis unveiled core pharmacological mechanisms,notably fluid shear stress and atherosclerosis,lipid and atherosclerosis,P13K-Akt,MAPK,and relaxin signaling pathways.Compounds 5–8 demonstrated robust interactions with multiple targets through hydrogen bonds,van der Waals forces,and pi-alkyl interactions,which serve as primary stabilizers of docking complexes.Notably,compound 7 exhibited promising ADMET prediction results,suggesting its potential for drug molecule development.Our findings underscored the synergistic effects of P.vulgaris on multiple targets and pathways in hypertension treatment,reflecting the characteristic multi-component and multi-target effects of TCM.
