Microplastics often co-occur with a variety of organic contaminants in aquatic environment and pose combined risks to aquatic wildlife. Here, we investigated joint effects of microsized polystyrene(mPS, 5 μm) and an ...Microplastics often co-occur with a variety of organic contaminants in aquatic environment and pose combined risks to aquatic wildlife. Here, we investigated joint effects of microsized polystyrene(mPS, 5 μm) and an organophosphate pesticide chlorpyrifos on zebrafish,using multiple endpoints at both fish individual and gut microbiota levels. It was revealed that mPS ingested by zebrafish accumulated in gut and liver, and caused oxidative stress, hyperactive swimming performance and histological damages in fish, and induced disorders and diversity alterations of the gut microbial community. More importantly, mPS exhibited considerable adsorption capacity against chlorpyrifos, and those adsorbing chlorpyrifos presented greater effects on fish individuals but no different effects on gut microbiota compared to single mPS exposure. Together with body residues of chlorpyrifos in zebrafish,it was proposed that the joint effects between mPS and chlorpyrifos were attributed to the chlorpyrifos released from mPS within zebrafish. The present results provided a comprehensive understanding of joint effects of mPS and contaminants co-occurring in the environment and emphasized the importance of considering the adsorbed chemicals in toxicological studies of microplastics.展开更多
基金supported by the National Natural Science Foundation of China (Nos. 21936004, 21806055)the Joint Funds of the National Natural Science Foundation of China (No. U1901220)+2 种基金the Guangdong Provincial Department of Science and Technology (No. 2019A1515011583)Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (No. SML2021SP208)Innovative Research Team of Department of Education of Guangdong Province (No. 2020KCXTD005)。
文摘Microplastics often co-occur with a variety of organic contaminants in aquatic environment and pose combined risks to aquatic wildlife. Here, we investigated joint effects of microsized polystyrene(mPS, 5 μm) and an organophosphate pesticide chlorpyrifos on zebrafish,using multiple endpoints at both fish individual and gut microbiota levels. It was revealed that mPS ingested by zebrafish accumulated in gut and liver, and caused oxidative stress, hyperactive swimming performance and histological damages in fish, and induced disorders and diversity alterations of the gut microbial community. More importantly, mPS exhibited considerable adsorption capacity against chlorpyrifos, and those adsorbing chlorpyrifos presented greater effects on fish individuals but no different effects on gut microbiota compared to single mPS exposure. Together with body residues of chlorpyrifos in zebrafish,it was proposed that the joint effects between mPS and chlorpyrifos were attributed to the chlorpyrifos released from mPS within zebrafish. The present results provided a comprehensive understanding of joint effects of mPS and contaminants co-occurring in the environment and emphasized the importance of considering the adsorbed chemicals in toxicological studies of microplastics.
