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Effects of hyaluronan oligosaccharides on apoptosis ofhuman gingival fibroblasts
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作者 yuki tanne Kotaro Tanimoto +4 位作者 Satoru Okuma Ryo Kunimatsu Naoto Hirose Tomomi Mitsuyoshi Kazuo tanne 《Open Journal of Stomatology》 2013年第1期19-24,共6页
An?increased?content?of?low?molecular?weight (LMW)- HA?was?found?in?human?gingiva?during?the?initial phase of?periodontitis. A?number?of?functions?have?been?described?for LMW-HA?in?inflammatory?processes.?It?is?also?d... An?increased?content?of?low?molecular?weight (LMW)- HA?was?found?in?human?gingiva?during?the?initial phase of?periodontitis. A?number?of?functions?have?been?described?for LMW-HA?in?inflammatory?processes.?It?is?also?demonstrated?that?LMWHA?induces?apoptosis?in?chondrocytes?and?tumor?cells,?however, effects of?LMWHA on the apoptosis?of?gingival?fibroblasts?(GFs) remain?unclear. The?purpose?of?this?study?was?to?investigate?the effects?of HA?oligosaccharides?(HAoligo) on?the apoptosis of GFs. GFs were?isolated?from?palatal?gingival?tissues around?the upper?first?premolars of eight youth?(6 -10?years?old)?and eight adult?(26 -35?years?old) subjects.?Cultured?GFs?were?subjected?to?the?analyses,?ELISA?for?cell?proliferation,?quantitative real?time-PCR?for?gene?expression?of?Bcl-2,?and?caspase-3?assay?for?apoptotic potency. Proliferative?activity?of?cultured?GFs was increased? 展开更多
关键词 HYALURONAN OLIGOSACCHARIDE APOPTOSIS CASPASE-3
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Longitudinal changes in the height and location of bone bridge from autogenous iliac bone graft in patients with cleft lip and palate
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作者 Kotaro Tanimoto yuki tanne +4 位作者 Keisuke Sumi Naoto Hirose Nobuhiko Kawai Eiji Tanaka Kazuo tanne 《Open Journal of Stomatology》 2013年第1期58-62,共5页
Background: Secondary alveolar bone graft has been widely used for the closure of maxillary bone defects in patients with cleft lip and palate (CLP). However, the log-term stability of grafted bone in detail remains u... Background: Secondary alveolar bone graft has been widely used for the closure of maxillary bone defects in patients with cleft lip and palate (CLP). However, the log-term stability of grafted bone in detail remains unclear. Purpose: To evaluate the nature of longitudinal changes in the grafted bone from autogenous iliac bone in patients with CLP for more than 2 years after the surgery. Methods and Subjects: The subjects were 124 CLP patients treated with iliac bone graft in Hiroshima University Hospital from 1997 to 2007. The height and location of the bone bridge was evaluated radiographically before and after surgery. Results: 1) Bone graft was performed at a mean age of 12.5 (±4.2) years old. The canines on the cleft side were not erupted at the surgery in 60.0%. All canines erupted except for the congenital missing and impacted ones (11.3%) within 2 years after bone graft;2) The height of the bone bridge was more than 11 mm in 71.8% of all patients 1 month after surgery. In addition, the location of bone bridge in 66.1% of all patients were clinically satisfactory. However, these rates decreased time-dependently owing to bone resorption after the bone graft. Conclusions: In conclusion, it is shown that autogenous bone graft produce a sufficient bone bridge in the jaw cleft area by the long-term evaluation of the changes in height and location of bone bridge, although some amount of time-dependent bone resorption is inevitable. 展开更多
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