Biliary atresia is a rare infant disease that predisposes patients to liver transplantation and death if not treated in time.However,early diagnosis is challenging because the clinical manifestations and laboratory te...Biliary atresia is a rare infant disease that predisposes patients to liver transplantation and death if not treated in time.However,early diagnosis is challenging because the clinical manifestations and laboratory tests of biliary atresia overlap with other cholestatic diseases.Therefore,it is very important to develop a simple,safe and reliable method for the early diagnosis of biliary atresia.Herein,a novel NIR-Ⅱfluorescence probe,HZL2,with high quantum yield,excellent biocompatibility,low cytotoxicity and rapid excretion through the liver and gallbladder was developed based on the oil/water partition coefficient and permeability.A simple fecal sample after injection of HZL2 can be used to efficiently identify the success of the mouse model of biliary atresia for the first time,allowing for an early diagnosis of the disease.This study not only developed a simple and safe method for the early diagnosis of biliary atresia with great potential in clinical translation but also provides a research tool for the development of pathogenesis and therapeutic medicines for biliary atresia.展开更多
Fluorescent NIR-Ⅱ imaging has a wide range of benefits not offered by other imaging modalities for biomedical applications, derived from its combination of high spatial and temporal resolution due to reduced photon a...Fluorescent NIR-Ⅱ imaging has a wide range of benefits not offered by other imaging modalities for biomedical applications, derived from its combination of high spatial and temporal resolution due to reduced photon absorption, scattering and tissue auto-fluorescence. Compared to the well-studied NIR-Ⅰ small-molecule fluorophores, the structures of NIR-Ⅱ fluorophores are scarce. To date, the main fluorophore units are composed of conjugated π system with a benzobisthiadiazole(BBTD) core and donor-acceptor-donor(D-A-D) structure. Herein, researchers Yang et al. and Zhang et al. have reported new NIR-Ⅱ probes ECX and FD-1080 respectively without a BBTD core which are highlighted.展开更多
Osteosarcoma is the most common primary malignant tumor of bone,particularly among children and adolescents.Advances in imaging,surgical techniques,and implants have dramatically reduced the need for amputation in the...Osteosarcoma is the most common primary malignant tumor of bone,particularly among children and adolescents.Advances in imaging,surgical techniques,and implants have dramatically reduced the need for amputation in the past three decades.Recently,in vivo fluorescence imaging in the second near-infrared window(NIR-II,1,000–1,700 nm)shows impressive advantages of deeper tissue penetration and higher spatial resolution,which makes it a promising tool for the early diagnosis and post-operative observation of Osteosarcoma.To the best of our knowledge,this paper is the first time to develop a novel NIR-II fluorescence probe conjugated with an osteosarcoma targeted oligopeptide for molecular tumor imaging in a xenograft orthotopic osteosarcoma mouse model.展开更多
Peptide-based probes play prominent roles in biomedical research due to their promising properties such as high biocompatibility,fast excretion, favorable pharmacokinetics as well as easy and robust preparation. Consi...Peptide-based probes play prominent roles in biomedical research due to their promising properties such as high biocompatibility,fast excretion, favorable pharmacokinetics as well as easy and robust preparation. Considering the translation of imaging probes into clinical applications, peptide-based probes remain to be the most desirable and optimal candidates. This review summarized the development of peptide-based probes with promising imaging modalities and highlighted the successful applications for in vivo biomedical imaging.展开更多
Ovarian cancer is a global problem,and is typically diagnosed in the middle or late stages,with a mysterious abdominal mass or atypical abdominal metastases due to the lack of specific initial diagnostic methods.Dual-...Ovarian cancer is a global problem,and is typically diagnosed in the middle or late stages,with a mysterious abdominal mass or atypical abdominal metastases due to the lack of specific initial diagnostic methods.Dual-modal near-infrared Ⅱ(NIR-Ⅱ,1,000–1,700 nm)fluorescence/photoacoustic imaging has great potential in early ovarian cancer diagnosis and image-guided surgery due to its high sensitivity and deep penetration.Herein,we report a novel organic NIR-Ⅱ dye(H10)with excellent aggregation-induced-emission(AIE)characteristics(I/I0>1.6)utilizing a selenadiazolo-[3,4-f]benzo[c][1,2,5]thiadiazole(ST)-based building block.Then,water-soluble and biocompatible H10@follicle-stimulating hormone(H10@FSH)dots with superior optical/photoacoustic properties and a tenfold increase in ovarian-specific targeting ability were synthesized.Finally,for the first time,in vivo dual-mode NIR-Ⅱ fluorescent/photoacoustic(PA)imaging and image-guided surgery of patient-derived tumor xenograft(PDTX)and micro-metastatic abdominal ovarian cancer lesions were investigated.This novel strategy will establish a new method for early detection of ovarian cancer and significantly improve the prognosis of ovarian cancer patients.展开更多
Accurate and dynamic visualization of vascular diseases can contribute to restraining further deterioration from diseases in a timely manner.However,visualization is still unable to precisely determine whether and to ...Accurate and dynamic visualization of vascular diseases can contribute to restraining further deterioration from diseases in a timely manner.However,visualization is still unable to precisely determine whether and to what extent blood vessels or brain tissues are damaged.Here,we report novel benzobis(1,2,5-thiadiazole)-based second near-infrared region(NIR-II)fluorophores HY1-HY4 with highly twisted structures(55°at the S_(0) state),extremely strong aggregation-induced emission(AIE)characteristics(I/I_(0)>13),and remarkably high fluorescence quantum yields(QYs)(up to 14.45%)in the NIR-II region(>1000 nm)and∼0.27%in the nearinfrared IIb window(NIR-IIb,>1500 nm)in aqueous solution.Using NIR-IIb AIE HY4 dots,high-resolution NIR-IIb fluorescence imaging of revascularization and thrombolysis,and real-time feedback of the therapeutic efficacy of Chinese medicine Dengzhan Xixin injection(DXI)on ischemic stroke,were achieved for the first time.In addition,results showed that DXI conferred neuroprotection against cerebral ischemia injury mediated via the angiogenesis pathway.These attractive results provide a new perspective for designing ultrabright NIR-IIb probes for vascular-related phenomena,disease assessment,and precise intraoperative imageguided therapy with a deeper tissue penetration depth and higher resolution.展开更多
基金partially supported by grants from the National Key R&D Program of China(2020YFA0908800)NSFC(82273796)+6 种基金Special Funds for Guiding Local Science and Technology Development of Central Government(XZ202202YD0021C,China)Tibet Autonomous Region COVID-19 Prevention and Control Programs for Science and Technology Development(XZ202201ZY0046G,China)Guangdong Basic and Applied Basic Research Foundation(2023A1515012649,China)Shenzhen Science and Technology Research Grant(JCYJ20220530140605012,China)Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University(ZNJC202209,China)Project Funded by China Postdoctoral Science Foundation(2022M712091,China)the Fundamental Research Funds for the Central Universities。
文摘Biliary atresia is a rare infant disease that predisposes patients to liver transplantation and death if not treated in time.However,early diagnosis is challenging because the clinical manifestations and laboratory tests of biliary atresia overlap with other cholestatic diseases.Therefore,it is very important to develop a simple,safe and reliable method for the early diagnosis of biliary atresia.Herein,a novel NIR-Ⅱfluorescence probe,HZL2,with high quantum yield,excellent biocompatibility,low cytotoxicity and rapid excretion through the liver and gallbladder was developed based on the oil/water partition coefficient and permeability.A simple fecal sample after injection of HZL2 can be used to efficiently identify the success of the mouse model of biliary atresia for the first time,allowing for an early diagnosis of the disease.This study not only developed a simple and safe method for the early diagnosis of biliary atresia with great potential in clinical translation but also provides a research tool for the development of pathogenesis and therapeutic medicines for biliary atresia.
文摘Fluorescent NIR-Ⅱ imaging has a wide range of benefits not offered by other imaging modalities for biomedical applications, derived from its combination of high spatial and temporal resolution due to reduced photon absorption, scattering and tissue auto-fluorescence. Compared to the well-studied NIR-Ⅰ small-molecule fluorophores, the structures of NIR-Ⅱ fluorophores are scarce. To date, the main fluorophore units are composed of conjugated π system with a benzobisthiadiazole(BBTD) core and donor-acceptor-donor(D-A-D) structure. Herein, researchers Yang et al. and Zhang et al. have reported new NIR-Ⅱ probes ECX and FD-1080 respectively without a BBTD core which are highlighted.
基金supported by the National Natural Science Foundation of China(81773674,21473041,81573383)Natural Science Foundation of Hubei Province(2017CFB711,2017CFA024,2016ACA126)+3 种基金the Fundamental Research Funds for the Central Universities,Tibet Autonomous Region Science and Technology Plan Project Key Project(XZ201901-GB-11)the Applied Basic Research Program of Wuhan Municipal Bureau of Science and Technology(2019020701011429)Project First-Class Disciplines Development Supported by Chengdu University of Traditional Chinese Medicine(CZYJC1903)Health Commission of Hubei Province Scientific Research Project(WJ2019M178,WJ2019M177)。
文摘Osteosarcoma is the most common primary malignant tumor of bone,particularly among children and adolescents.Advances in imaging,surgical techniques,and implants have dramatically reduced the need for amputation in the past three decades.Recently,in vivo fluorescence imaging in the second near-infrared window(NIR-II,1,000–1,700 nm)shows impressive advantages of deeper tissue penetration and higher spatial resolution,which makes it a promising tool for the early diagnosis and post-operative observation of Osteosarcoma.To the best of our knowledge,this paper is the first time to develop a novel NIR-II fluorescence probe conjugated with an osteosarcoma targeted oligopeptide for molecular tumor imaging in a xenograft orthotopic osteosarcoma mouse model.
基金partially supported by grants from the National Natural Science Foundation of China(NSFC Nos. 21708012,81773674, 81573383,21390402, 81725009, 21788102, 81425015)111 Project (No. B17019)+6 种基金NKR&DPC (No. 2016YFA00900)NSFHP(Nos. 2017CFB151, 2017CFA024, 2017CFB711, 2016ACA126)ABRPSTCS (No. SYG201521)NSFJP (No. BK20160387)Shenzhen Science and Technology Research Grant (No. JCYJ20170303170809222)self-determined research funds of CCNU from the colleges, basic research and operation of MOE for the Central Universities (No. 23020205170469)Wuhan Morning Light Plan of Youth Science and Technology (No. 201705304010321)
文摘Peptide-based probes play prominent roles in biomedical research due to their promising properties such as high biocompatibility,fast excretion, favorable pharmacokinetics as well as easy and robust preparation. Considering the translation of imaging probes into clinical applications, peptide-based probes remain to be the most desirable and optimal candidates. This review summarized the development of peptide-based probes with promising imaging modalities and highlighted the successful applications for in vivo biomedical imaging.
基金supported by the National Key Research and development Program of China(Nos.2020YFA0908800 and 2015DFA30440)the National Natural Science Foundation of China(Nos.82111530209,81773674,81573383,91959103,and 61971447)+4 种基金Hubei Province Scientific and Technical Innovation Key Project(No.2020BAB058)Beijing Natural Science Foundation(No.JQ18023)Shenzhen Science and Technology Research Grant(No.JCYJ20190808152019182)the Local Development Funds of Science and Technology Department of Tibet(Nos.XZ202102YD0033C and XZ202202YD0021C)the Fundamental Research Funds for the Central Universities.
文摘Ovarian cancer is a global problem,and is typically diagnosed in the middle or late stages,with a mysterious abdominal mass or atypical abdominal metastases due to the lack of specific initial diagnostic methods.Dual-modal near-infrared Ⅱ(NIR-Ⅱ,1,000–1,700 nm)fluorescence/photoacoustic imaging has great potential in early ovarian cancer diagnosis and image-guided surgery due to its high sensitivity and deep penetration.Herein,we report a novel organic NIR-Ⅱ dye(H10)with excellent aggregation-induced-emission(AIE)characteristics(I/I0>1.6)utilizing a selenadiazolo-[3,4-f]benzo[c][1,2,5]thiadiazole(ST)-based building block.Then,water-soluble and biocompatible H10@follicle-stimulating hormone(H10@FSH)dots with superior optical/photoacoustic properties and a tenfold increase in ovarian-specific targeting ability were synthesized.Finally,for the first time,in vivo dual-mode NIR-Ⅱ fluorescent/photoacoustic(PA)imaging and image-guided surgery of patient-derived tumor xenograft(PDTX)and micro-metastatic abdominal ovarian cancer lesions were investigated.This novel strategy will establish a new method for early detection of ovarian cancer and significantly improve the prognosis of ovarian cancer patients.
基金This work was partially supported by grants from the National Key R&D Program of China(no.2020YFA09-08800)NSFC(nos.81773674,81573383,21763002,82111530209,and 91959103)+5 种基金Shenzhen Science and Technology Research Grant(no.JCYJ20190808152019182)Hubei Province Scientific and Technical Innovation Key Project(no.2020BAB058),the Applied Basic Research Program of Wuhan Municipal Bureau of Science and Technology(no.2019020701011429)the Major Science and Technology Project of Sichuan Science and Technology Department(no.2019YFSY0046)Project First-Class Disciplines Development Supported by Chengdu University of Traditional Chinese Medicine(no.CZYJC1903)Tibet Autonomous Region Science and Technology Plan Project Key Project(no.XZ201901-GB-11)the Local Development Funds of Science andTechnology Department of Tibet(nos.XZ202102YD0033C and XZ202001YD0028C),and the Fundamental Research Funds for the Central Universities.
文摘Accurate and dynamic visualization of vascular diseases can contribute to restraining further deterioration from diseases in a timely manner.However,visualization is still unable to precisely determine whether and to what extent blood vessels or brain tissues are damaged.Here,we report novel benzobis(1,2,5-thiadiazole)-based second near-infrared region(NIR-II)fluorophores HY1-HY4 with highly twisted structures(55°at the S_(0) state),extremely strong aggregation-induced emission(AIE)characteristics(I/I_(0)>13),and remarkably high fluorescence quantum yields(QYs)(up to 14.45%)in the NIR-II region(>1000 nm)and∼0.27%in the nearinfrared IIb window(NIR-IIb,>1500 nm)in aqueous solution.Using NIR-IIb AIE HY4 dots,high-resolution NIR-IIb fluorescence imaging of revascularization and thrombolysis,and real-time feedback of the therapeutic efficacy of Chinese medicine Dengzhan Xixin injection(DXI)on ischemic stroke,were achieved for the first time.In addition,results showed that DXI conferred neuroprotection against cerebral ischemia injury mediated via the angiogenesis pathway.These attractive results provide a new perspective for designing ultrabright NIR-IIb probes for vascular-related phenomena,disease assessment,and precise intraoperative imageguided therapy with a deeper tissue penetration depth and higher resolution.
