To identify cytoplasmic male sterility(CMS)-associated genes in tomato,we determined the genome sequences of mitochondria and chloroplasts in three CMS tomato lines derived from independent asymmetric cell fusions,the...To identify cytoplasmic male sterility(CMS)-associated genes in tomato,we determined the genome sequences of mitochondria and chloroplasts in three CMS tomato lines derived from independent asymmetric cell fusions,their nuclear and cytoplasmic donors,and male fertile weedy cultivated tomato and wild relatives.The structures of the CMS mitochondrial genomes were highly divergent from those of the nuclear and cytoplasmic donors,and genes of the donors were mixed up in these genomes.On the other hand,the structures of CMS chloroplast genomes were moderately conserved across the donors,but CMS chloroplast genes were unexpectedly likely derived from the nuclear donors.Comparative analysis of the structures and contents of organelle genes and transcriptome analysis identified three genes that were uniquely present in the CMS lines,but not in the donor or fertile lines.RNA-sequencing analysis indicated that these three genes transcriptionally expressed in anther,and identified different RNA editing levels in one gene,orf265,that was partially similar to ATP synthase subunit 8,between fertile and sterile lines.The orf265 was a highly potential candidate for CMS-associated gene.This study suggests that organelle reorganization mechanisms after cell fusion events differ between mitochondria and chloroplasts,and provides insight into the development of new F1 hybrid breeding programs employing the CMS system in tomato.展开更多
基金This work was supported by the Kazusa DNA Research Institute Foundation to K.S.the Project of the NARO Bio-oriented Technology Research Advancement Institution(Research Program on Development of Innovative Technology,Grant numbers:30010A and ID21448196)to K.S.,Y.M.+2 种基金T.A.,JSPS KAKENHI(Grant Numbers:17H03761 and 21H02181)to T.A.JSPS Research Fellowships for Young Researchers(Grant Number:21J20479)to K.KSeeds of Micro-Tom(TOMJPF0001)was provided from National BioResource Project Tomato(NBRP tomato).
文摘To identify cytoplasmic male sterility(CMS)-associated genes in tomato,we determined the genome sequences of mitochondria and chloroplasts in three CMS tomato lines derived from independent asymmetric cell fusions,their nuclear and cytoplasmic donors,and male fertile weedy cultivated tomato and wild relatives.The structures of the CMS mitochondrial genomes were highly divergent from those of the nuclear and cytoplasmic donors,and genes of the donors were mixed up in these genomes.On the other hand,the structures of CMS chloroplast genomes were moderately conserved across the donors,but CMS chloroplast genes were unexpectedly likely derived from the nuclear donors.Comparative analysis of the structures and contents of organelle genes and transcriptome analysis identified three genes that were uniquely present in the CMS lines,but not in the donor or fertile lines.RNA-sequencing analysis indicated that these three genes transcriptionally expressed in anther,and identified different RNA editing levels in one gene,orf265,that was partially similar to ATP synthase subunit 8,between fertile and sterile lines.The orf265 was a highly potential candidate for CMS-associated gene.This study suggests that organelle reorganization mechanisms after cell fusion events differ between mitochondria and chloroplasts,and provides insight into the development of new F1 hybrid breeding programs employing the CMS system in tomato.
