By using the characteristic that poly (N-isopropylacrylamide) can be adhered to callulose acetais-nitrate membrane in high retentior tightly and ramdly,we developed a novel immunoamay method- polymer enayme Hnked immu...By using the characteristic that poly (N-isopropylacrylamide) can be adhered to callulose acetais-nitrate membrane in high retentior tightly and ramdly,we developed a novel immunoamay method- polymer enayme Hnked immunoblotting amay method.Human serum HBsAg can detected as little as 10ng/ml with this immunoassay system.展开更多
A new method for the study of triplex DNA is established according the fluorescence enhancement of molecular 揕ight Switch?complex of Ru(phen)2(dppx)2+ when it intercalate into triplex DNA. Because the fluorescence i...A new method for the study of triplex DNA is established according the fluorescence enhancement of molecular 揕ight Switch?complex of Ru(phen)2(dppx)2+ when it intercalate into triplex DNA. Because the fluorescence intensity of Ru(phen)2(dppx)2+ bonded to triplex DNA is in the case higher than that bonded to duplex DNA in certain range of DNA concentration, the method is much more sensitive than other methods reported previously.展开更多
文摘By using the characteristic that poly (N-isopropylacrylamide) can be adhered to callulose acetais-nitrate membrane in high retentior tightly and ramdly,we developed a novel immunoamay method- polymer enayme Hnked immunoblotting amay method.Human serum HBsAg can detected as little as 10ng/ml with this immunoassay system.
基金the support of Alexander von Humboldt FoundationCAS K.C.Wong post-doctoral Research award fund+1 种基金Natural Science Foundation of China for postdoctoral fellowship Educational Ministry of Chinathe National Natural Science Foundation of China(29605001),Wuhan University and its Alumni Association.
文摘A new method for the study of triplex DNA is established according the fluorescence enhancement of molecular 揕ight Switch?complex of Ru(phen)2(dppx)2+ when it intercalate into triplex DNA. Because the fluorescence intensity of Ru(phen)2(dppx)2+ bonded to triplex DNA is in the case higher than that bonded to duplex DNA in certain range of DNA concentration, the method is much more sensitive than other methods reported previously.
