Notopterygium incisum C. C. Ting ex H. T. Chang(Apiaceae) is an endangered perennial herb in China. The lack of transcriptomic and genomic resources for N. incisum greatly hinders studies of its population genetics an...Notopterygium incisum C. C. Ting ex H. T. Chang(Apiaceae) is an endangered perennial herb in China. The lack of transcriptomic and genomic resources for N. incisum greatly hinders studies of its population genetics and conservation. In this study, we employed RNA-seq technology to characterize transcriptomes for the flowers, leaves, and stems of this endangered herb. A total of 56 million clean reads were assembled into 120,716 unigenes with an N50 length of 850 bp. Among these unigenes, 70,245(58.19%) were successfully annotated and 65,965(54.64%) were identified as coding sequences based on their similarities with sequences in public databases. We identified 21 unigenes that had significant relationships with cold tolerance in N. incisum according to gene ontology(GO) annotation analysis. In addition, 13,149 simple sequence repeats(SSRs) and 85,681 single nucleotide polymorphisms were detected as potential molecular genetic markers. Ninety-six primer pairs of SSRs were randomly selected to validate their amplification efficiency and polymorphism. Nineteen SSR loci exhibited polymorphism in three natural populations of N. incisum. These results provide valuable resources to facilitate future functional genomics and conservation genetics studies of N. incisum.展开更多
Soil-rock mixture(SRM)filling in fault zone is an inhomogeneous geomaterial,which is composed of soil and rock block.It controls the deformation and stability of the abutment and dam foundation,and threatens the long-...Soil-rock mixture(SRM)filling in fault zone is an inhomogeneous geomaterial,which is composed of soil and rock block.It controls the deformation and stability of the abutment and dam foundation,and threatens the long-term safety of high arch dams.To study the macroscopic and mesoscopic mechanical properties of SRM,the development of a viable mesoscopic numerical simulation method with a mesoscopic model generation technology,and a reasonable parametric model is crucially desired to overcome the limitations of experimental conditions,specimen dimensions,and experiment fund.To this end,this study presents a mesoscopic numerical method for simulating the mechanical behavior of SRM by proposing mesoscopic model generation technology based on its mesostructure features,and a rock parameter model considering size effect.The validity and rationality of the presented mesoscopic numerical method is experimentally verified by the triaxial compression tests with different rock block contents(RBC).The results indicate that the rock block can increase the strength of SRM,and it is proved that the random generation technique and the rock parameter model considering size effect are validated.Furthermore,there are multiple failure surfaces for inhomogeneous geomaterial of SRM,and the angle of the failure zone is no longer 45◦.The yielding zones of the specimen are more likely to occur in thin sections of soil matrix isolated by blocks with the failure path avoiding the rock block.The proposed numerical method is effective to investigate the meso-damage mechanism of SRM.展开更多
Objective:To evaluate the efficacy and safety of Tongxuekang capsule in the treatment of knee osteoarthritis.Methods:Single-centred,open selfcontrol methods before and after administration were performed in different ...Objective:To evaluate the efficacy and safety of Tongxuekang capsule in the treatment of knee osteoarthritis.Methods:Single-centred,open selfcontrol methods before and after administration were performed in different time length of 14 days,28 days,56 days and 84 days to observe for improvement by evaluating knee joint pain scores,WOMAC scores,and doctors and patients’scores on the overall condition of the disease before and after treatment.Results:A total of 34 patients with primary knee osteoarthritis from October 2016 to September 2017 were enrolled in Xijing Hospital.There were 7 males and 27 females with an average age of 54 years and an average body mass index of 25kg/m2.Joint pain scores,WOMAC scores,and doctors’and patients’overall scores for the disease were significantly improved compared to prior treatment,with no significant adverse reactions in all patients.Conclusion:Tongxuekang capsule is safe and effective in the treatment of knee osteoarthritis and thus,can be used as a treatment option.In order to conduct further studies on the effectiveness and safety of consumption with the use of the capsule in a larger population,an increase in the sample size of the current study is necessary.展开更多
Herein,styryl phosphonate monoester(SPE) was synthesized and first introduced as rare earth extractant.The solvent extraction of lanthanum(Ⅲ) from nitrate solution using styryl phosphonate mono-isooctyl ester(SPE108)...Herein,styryl phosphonate monoester(SPE) was synthesized and first introduced as rare earth extractant.The solvent extraction of lanthanum(Ⅲ) from nitrate solution using styryl phosphonate mono-isooctyl ester(SPE108),di-2-ethylhexyl phosphoric acid(D2 EHPA) and 2-ethylhexyl phosphonic acidmono-2-ethylhexyl ester(EHEHPA) as extractants was investigated.The effects of experimental parameters including equilibrium time,extractant concentration,aqueous pH,phase ratio and salt concentration on the extraction process were studied.The results indicate that the extraction ability and capacity of the extractants follow the order:SPE108> D2 EHPA> EHEHPA.What’s more,the extraction process is less affected by ammonium sulfate in the aqueous phase with SPE108.The results of the separation between lanthanum and adjacent lanthanides(Ce,Pr,Nd,Sm) show that SPE108 can separate lanthanides efficiently at low pH.The extraction mechanism of SPE108 is proved to be similar to D2 EHPA,and the density functional theory(DFT) calculation results infer that SPE108 exhibits superior extraction ability due to its strong electron-accepting ability.展开更多
The present paper presents analysis of the influence of the soil saturation on the free-field response of liquefiable soils.Analyses are conducted using a finite element program developed for partially saturated soils...The present paper presents analysis of the influence of the soil saturation on the free-field response of liquefiable soils.Analyses are conducted using a finite element program developed for partially saturated soils subjected to cyclic loading.The performances of the proposed model are analysed by simulation of undrained triaxial tests with different water saturations.The finite element program is then used for the analysis of the influences of the soil saturation,density and position of the water table on the liquefaction of a soil layer subjected to cyclic loading.Results show that the soil saturation significantly affects the liquefaction of partially saturated sandy layers.The decrease in the soil saturation results in a reduction of liquefaction risk.展开更多
基金supported by the National Natural Science Foundation of China (31470400)Shaanxi Provincial Key Laboratory Project of Department of Education (grant no. 17JS135)+2 种基金the Shaanxi Provincial Education Department Serves Local Special Projects (grant no. 2018JC032)the programme for the Key Research and Development Plan in Shaanxi province (grant no. 2018ZDXM-SF-014)Public health specialty in the Department of Traditional Chinese Medicine (grants no. 2011-76, 201207002, 201213, 2013-135, 201407002, 2014-76, 2015-78, 2016-44, 2017-66)
文摘Notopterygium incisum C. C. Ting ex H. T. Chang(Apiaceae) is an endangered perennial herb in China. The lack of transcriptomic and genomic resources for N. incisum greatly hinders studies of its population genetics and conservation. In this study, we employed RNA-seq technology to characterize transcriptomes for the flowers, leaves, and stems of this endangered herb. A total of 56 million clean reads were assembled into 120,716 unigenes with an N50 length of 850 bp. Among these unigenes, 70,245(58.19%) were successfully annotated and 65,965(54.64%) were identified as coding sequences based on their similarities with sequences in public databases. We identified 21 unigenes that had significant relationships with cold tolerance in N. incisum according to gene ontology(GO) annotation analysis. In addition, 13,149 simple sequence repeats(SSRs) and 85,681 single nucleotide polymorphisms were detected as potential molecular genetic markers. Ninety-six primer pairs of SSRs were randomly selected to validate their amplification efficiency and polymorphism. Nineteen SSR loci exhibited polymorphism in three natural populations of N. incisum. These results provide valuable resources to facilitate future functional genomics and conservation genetics studies of N. incisum.
基金supported by the Chinese National Natural Science Foundation(51739006)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(SJKY19_0433)the Fundamental Research Funds for the Central Universities(2019B65714).
文摘Soil-rock mixture(SRM)filling in fault zone is an inhomogeneous geomaterial,which is composed of soil and rock block.It controls the deformation and stability of the abutment and dam foundation,and threatens the long-term safety of high arch dams.To study the macroscopic and mesoscopic mechanical properties of SRM,the development of a viable mesoscopic numerical simulation method with a mesoscopic model generation technology,and a reasonable parametric model is crucially desired to overcome the limitations of experimental conditions,specimen dimensions,and experiment fund.To this end,this study presents a mesoscopic numerical method for simulating the mechanical behavior of SRM by proposing mesoscopic model generation technology based on its mesostructure features,and a rock parameter model considering size effect.The validity and rationality of the presented mesoscopic numerical method is experimentally verified by the triaxial compression tests with different rock block contents(RBC).The results indicate that the rock block can increase the strength of SRM,and it is proved that the random generation technique and the rock parameter model considering size effect are validated.Furthermore,there are multiple failure surfaces for inhomogeneous geomaterial of SRM,and the angle of the failure zone is no longer 45◦.The yielding zones of the specimen are more likely to occur in thin sections of soil matrix isolated by blocks with the failure path avoiding the rock block.The proposed numerical method is effective to investigate the meso-damage mechanism of SRM.
文摘Objective:To evaluate the efficacy and safety of Tongxuekang capsule in the treatment of knee osteoarthritis.Methods:Single-centred,open selfcontrol methods before and after administration were performed in different time length of 14 days,28 days,56 days and 84 days to observe for improvement by evaluating knee joint pain scores,WOMAC scores,and doctors and patients’scores on the overall condition of the disease before and after treatment.Results:A total of 34 patients with primary knee osteoarthritis from October 2016 to September 2017 were enrolled in Xijing Hospital.There were 7 males and 27 females with an average age of 54 years and an average body mass index of 25kg/m2.Joint pain scores,WOMAC scores,and doctors’and patients’overall scores for the disease were significantly improved compared to prior treatment,with no significant adverse reactions in all patients.Conclusion:Tongxuekang capsule is safe and effective in the treatment of knee osteoarthritis and thus,can be used as a treatment option.In order to conduct further studies on the effectiveness and safety of consumption with the use of the capsule in a larger population,an increase in the sample size of the current study is necessary.
基金National High Technology Research and Development Program of China(863 Program,2013AA064102)Hunan Provincial Science and Technology Plan Project,China (2016TP1007)。
文摘Herein,styryl phosphonate monoester(SPE) was synthesized and first introduced as rare earth extractant.The solvent extraction of lanthanum(Ⅲ) from nitrate solution using styryl phosphonate mono-isooctyl ester(SPE108),di-2-ethylhexyl phosphoric acid(D2 EHPA) and 2-ethylhexyl phosphonic acidmono-2-ethylhexyl ester(EHEHPA) as extractants was investigated.The effects of experimental parameters including equilibrium time,extractant concentration,aqueous pH,phase ratio and salt concentration on the extraction process were studied.The results indicate that the extraction ability and capacity of the extractants follow the order:SPE108> D2 EHPA> EHEHPA.What’s more,the extraction process is less affected by ammonium sulfate in the aqueous phase with SPE108.The results of the separation between lanthanum and adjacent lanthanides(Ce,Pr,Nd,Sm) show that SPE108 can separate lanthanides efficiently at low pH.The extraction mechanism of SPE108 is proved to be similar to D2 EHPA,and the density functional theory(DFT) calculation results infer that SPE108 exhibits superior extraction ability due to its strong electron-accepting ability.
文摘The present paper presents analysis of the influence of the soil saturation on the free-field response of liquefiable soils.Analyses are conducted using a finite element program developed for partially saturated soils subjected to cyclic loading.The performances of the proposed model are analysed by simulation of undrained triaxial tests with different water saturations.The finite element program is then used for the analysis of the influences of the soil saturation,density and position of the water table on the liquefaction of a soil layer subjected to cyclic loading.Results show that the soil saturation significantly affects the liquefaction of partially saturated sandy layers.The decrease in the soil saturation results in a reduction of liquefaction risk.
