Correlation of visceral adipose tissue content with insulin signal transduction, lipid metabolism features and adipocytokine secretion in patients with T2DM

Objective: To study the correlation of visceral adipose tissue content with insulin signal transduction, lipid metabolism features and adipocytokine secretion in patients with type 2 diabetes mellitus (T2DM). Methods: The patients who were first diagnosed as T2DM in our hospital between March 2015 and February 2018 were selected as the T2DM group, and the healthy subjects who underwent physical examination during the same period were selected as the control group. The T2DM group underwent visceral adipose tissue content scanning and were divided into those with VAT 100 cm2 and those with VAT<100 cm2. Fasting peripheral blood was collected from the two groups to measure the F-Ins, lipid metabolism indexes and adipocytokines and calculate the HOMA-IR and ISI. Results: F-Ins and HOMA-IR levels as well as LDL-C, Apo-B, FFA, LP and RBP4 contents of T2DM group were significantly higher than those of control group whereas ISI level as well as HDL-C, Apo-A, APN and Omentin-1 contents was significantly lower than those of control group;F-Ins and HOMA-IR levels as well as of LDL-C, Apo-B, FFA, LP and RBP4 contents of T2DM group of patients with VAT 100 cm2 were higher than those of patients with VAT<100 cm2 whereas ISI level as well as HDL-C, Apo-A, APN and Omentin-1 contents was lower than those of patients with VAT<100 cm2. Conclusion: The increase of visceral adipose tissue content in patients with T2DM can aggravate the insulin resistance and cause the disorder of lipid metabolism and adipocytokine secretion.

Correlation of serum Leptin and Vaspin contents with renal function, oxidative stress and inflammatory response in patients with diabetic nephropathy

Objective: To investigate the correlation of serum Leptin and Vaspin contents with renal function, oxidative stress and inflammatory response in patients with diabetic nephropathy (DN). Methods: A total of 109 patients with type 2 diabetes mellitus alone and 76 patients with diabetic nephropathy were selected as diabetes group and DN group, and 100 healthy volunteers who underwent physical examination in our hospital were selected as normal control group. The differences in serum Leptin and Vaspin contents as well as renal function index, oxidative stress index and inflammatory mediator levels were compared among the three groups, and the internal relationship of serum Leptin and Vaspin contents in DN patients with renal function injury, oxidative stress and inflammatory reaction were further analyzed. Results: Serum Leptin and Vaspin contents of diabetes group and DN group were higher than those of normal control group, and serum Leptin and Vaspin contents of DN group were higher. Peripheral blood renal function indexes serum creatinine (SCr), blood urea nitrogen (BUN), β2 microglobulin (β2-MG) and cystatin-C (Cys-C) levels of DN group were higher than those of diabetes group and normal control group;oxidative stress indexes reactive oxygen species (ROS) and reactive nitrogen species (RNS) contents were higher than those of diabetes group and normal control group whereas total antioxidant capacity (T-AOC) content was lower than that of diabetes group and normal control group;inflammatory mediators transforming growth factor-β (TGF-β), interleukin-1β (IL-1β), interleukin-18 (IL-18), monocyte chemoattractant protein-1 (MCP-1) and intercellular adhesion molecules-1 (ICAM-1) contents were higher than those of diabetes group and normal control group. Correlation analysis showed that serum Leptin and Vaspin contents in DN patients were positively correlated with the degree of renal function injury, oxidative stress and inflammatory response. Conclusion:Serum Leptin and Vaspin contents abnormally increase in patients with DN, and their specific contents can objectively reflect the degree of renal function injury and inflammation stress.