闭角型青光眼患者红细胞免疫功能与EPO和ET-1的相关性

目的:探讨闭角型青光眼患者红细胞免疫功能与血清促红细胞生成素(EPO)和血浆内皮素-1(ET-1)的相关性。方法:选取2017-06/10期间我院收治的闭角型青光眼患者30例(病例组)与眼部正常者30例(对照组)为研究对象。测定并比较两组受试者红细胞免疫功能、血清EPO和血浆ET-1浓度,分析之间的相关性。结果:病例组受试者红细胞C3b受体花环率明显低于对照组(10.81%±2.01%vs 18.06%±3.44%),红细胞免疫复合物花环率明显高于对照组(17.21±3.49%vs 11.74±2.14%),血清EPO浓度明显高于对照组(26.10±5.22 mU/mL vs 22.68±4.06mU/mL),血浆ET-1浓度明显高于对照组(70.85±7.16ng/L vs 58.43±5.09ng/L)。闭角型青光眼患者红细胞C3b受体花环率与血清EPO浓度呈显著正相关(r=0.271,P<0.05),与血浆ET-1浓度无明显相关性。结论:闭角型青光眼患者红细胞免疫功能与血清EPO浓度呈正相关。

复杂性外伤性和非外伤性视网膜脱离视力恢复相关因素的分析

目的:探究复杂性外伤性和非外伤性视网膜脱离术后视力恢复的相关影响因素。方法:根据患者入院前患眼是否有外伤史,将135例复杂性视网膜脱离患者分为外伤组(66例66眼)和非外伤组(69例69眼)。比较两组患者行玻璃体视网膜手术治疗后视力恢复效果及并发症发生情况;采用Logistic回归分析法分析影响两组患者术后视力恢复的相关因素。结果:术后24h,外伤组和非外伤组患者视力恢复率(77.3%和78.3%)差异无统计学意义(P>0.05)。术后随访3mo,两组患者并发症发生率(18.2%和17.4%)差异无统计学意义(P>0.05)。多因素Logistic回归分析结果显示,年龄、损伤类型、视网膜脱离时间、视网膜脱离范围、术前合并玻璃体积血以及增生性玻璃体视网膜病变与外伤性视网膜脱离患者术后视力恢复显著相关(P<0.05);年龄、视网膜脱离时间、视网膜脱离范围以及黄斑状态与非外伤性视网膜脱离患者术后视力恢复显著相关(P<0.05)。结论:年龄、视网膜脱离时间、视网膜脱离范围与外伤性和非外伤性视网膜脱离患者视力恢复情况显著相关;损伤类型、术前合并玻璃体积血及增生性玻璃体视网膜病变与外伤性视网膜脱离患者视力恢复情况显著相关;黄斑状态与非外伤性视网膜脱离患者视力恢复情况显著相关。

Structure analysis and function prediction of human P2X7R based on bioinformatics

Objective:To analyze the structural features of human P2X7R protein,meanwhile homology,protein-protein interaction,and cell epitope were predicted to provide theoretical basis for the diagnosis and treatment of related diseases.Methods:Human P2X7R protein was analyzed and predicted by ProtParam、TMHMM、ProtScale and SignalP 5.0 Server et al.Results:Human P2X7R protein is composed of 595 amino acids,the molecular formula is C 3077 H 4749 N 835 O 871 S 40,with the theoretical isoelectric point PI of 8.62,the instability coefficient is 46.07,the total average hydrophilicity is-0.317,and the protein is an hydrophilic protein instead of secreted protein,no any signal peptide,but transmembrane region is contained,which belong to transmembrane protein.There are 157αhelices and 288 random coils in the secondary structure of human P2X7R protein,accounting for 26.39%and 48.40%of all amino acids,The coverage rate of the three-level structure prediction results is 100%,and the sequence consistency is 80.34%,and the ramachandran plot show that the predicted structure is relatively stability.The interaction of proteins show that there are a variety of proteins interacting with human P2X7R,and P2X7R was closely related to inflammation;this protein had many B and T cell epitopes,which could provide ideas for vaccine development.Conclusion:The results indicate that the analysis of human P2X7R protein that is based on bioinformatics method has certain reliability.P2X7R is involved in the development of inflammatory diseases,and the antigenic epitope of B,Th and CTL cells screened out by prediction will provide the treatment scheme for diseases.