Role of bone marrow-derived mesenchymal stem cells in a rat model of severe acute pancreatitis

AIM:To investigate the role and potential mechanisms of bone marrow mesenchymal stem cells(MSCs) in severe acute peritonitis(SAP).METHODS:Pancreatic acinar cells from Sprague Dawley rats were randomly divided into three groups:nonsodium deoxycholate(SDOC) group(non-SODC group),SDOC group,and a MSCs intervention group(i.e.,a co-culture system of MSCs and pancreatic acinar cells + SDOC).The cell survival rate,the concentration of malonaldehyde(MDA),the density of superoxide dismutase(SOD),serum amylase(AMS) secretion rate and lactate dehydrogenase(LDH) leakage rate were detected at various time points.In a separate study,Sprague Dawley rats were randomly divided into either an SAP group or an SAP + MSCs group.Serum AMS,MDA and SOD,interleukin(IL)-6,IL-10,and tumor necrosis factor(TNF)-α levels,intestinal mucosa injury scores and proliferating cells of small intestinal mucosa were measured at various time points after injecting either MSCs or saline into rats.In both studies,the protective effect of MSCs was evaluated.RESULTS:In vitro,The cell survival rate of pancreatic acinar cells and the density of SOD were significantly reduced,and the concentration of MDA,AMS secretion rate and LDH leakage rate were significantly increased in the SDOC group compared with the MSCs intervention group and the Non-SDOC group at each time point.In vivo,Serum AMS,IL-6,TNF-α and MAD level in the SAP + MSCs group were lower than the SAP group;however serum IL-10 level was higher than the SAP group.Serum SOD level was higher than the SAP group at each time point,whereas a significant betweengroup difference in SOD level was only noted after 24 h.Intestinal mucosa injury scores was significantly reduced and the proliferating cells of small intestinal mucosa became obvious after injecting MSCs.CONCLUSION:MSCs can effectively relieve injury to pancreatic acinar cells and small intestinal epithelium,promote the proliferation of enteric epithelium and repair of the mucosa,attenuate systemic inflammation in rats with SAP.

Clinical characteristics and risk factors of infantile hemangioma: a case-control study in Northwest China

Objective To investigate the clinical characteristics and risk factors of infantile hemangioma in Xinjiang Uygur Autonomous Region,China.Methods A total of 941 infants with infantile hemangioma were identified as subjects and matched with 941 infants of the same age without infantile hemangioma.Trained investigators used a standardized questionnaire to collect data from both groups,including demographic,prenatal,and perinatal characteristics.Univariate and multiple logistic regression models were used to screen risk factors.Results This study revealed that female (P < 0.001,OR =3.08,95% CI =2.54-3.74),maternal tea drinking (P< 0.001,OR =1.86,95% CI=1.32-2.61),low birthweight (P< 0.001,OR =3.01,95% CI=2.10-4.30),preterm (P< 0.001,OR =4.07,95% CI=2.73-6.09),gestational diabetes (P < 0.001,OR =2.03,95% CI =1.41-2.94),maternal progesterone use (P <0.001,OR =2.44,95% CI =1.83-3.25) and a history of hemangiomas in first-degree relatives (P <0.001,OR =1.80,95% CI =1.33-2.43) influenced the occurrence of infantile hemangioma.Female (P < 0.001,OR =2.82,95% CI =2.30-3.45),maternal progesterone use (P < 0.001,OR =14.64,95%CI =3.52-60.88) and a history of hemangiomas in first-degree relatives (P< 0.001,OR =0.011,95%CI =0.002-0.065) were independent risk factors for infantile hemangioma.Conclusions Female,maternal tea drinking,low birthweight,preterm,gestational diabetes,maternal progesterone use,and a history of hemangiomas in first-degree relatives may interact with each other leading to the occurrence of infantile hemangioma.

Analysis of Clinical Features of Patients With Psoriasis and Metabolic Syndrome in Xinjiang,China:A Cross-Sectional Study

Objective:Psoriasis is often closely related to metabolic syndrome (MS),herein we conducted this study to investigate the clinical characteristics of a large sample of psoriasis patients with MS in Xinjiang,China.Methods:The prevalence of MS,age,height,weight,body mass index (BMI),red blood cell count,white blood cell count,platelet count,aspartate aminotransferase level,alanine aminotransferase level,and other clinical data were analyzed in 2,492 patients with psoriasis.The patients were divided into 2 groups based on the presence of MS and their clinical features were compared.Studentt test was used for independent samples,and the chi-square test was used for count data.Results:Among the 2,492 patients,349 had MS and 1,269 were considered overweight/obese.There were significant differences in sex,age,disease course,age at onset,BMI,systolic blood pressure (SBP),and diastolic blood pressure (DBP) between the patients with MS and those without (allP < 0.05).The red blood cell count (P < 0.001),white blood cell count (P = 0.013),erythrocyte sedimentation rate (P < 0.001),blood urea nitrogen level (P < 0.001),triglyceride level (P < 0.001),total cholesterol level (P < 0.001),and low-density lipoprotein cholesterol level (P = 0.002) were significantly higher in the MS group than in the psoriasis-only group.The high-density lipoprotein cholesterol level was significantly lower in the MS group than in the psoriasis-only group (P < 0.001).The alanine aminotransferase (P = 0.145) and albumin levels (P = 0.192) were not significantly different between the 2 groups (P > 0.05).Binary logistic regression analysis showed that sex,age,BMI,SBP,DBP,and fasting plasma glucose were independent risk factors for MS in patients with psoriasis,and high-density lipoprotein cholesterol was considered a protective factor for these patients.Conclusion:The prevalence of MS in patients with psoriasis is high.Female sex,age,BMI,SBP,DBP,and fasting plasma glucose may be risk factors for psoriasis complicated by MS.