Anthocyanins are secondary metabolites in land plants that contribute to the colors of leaves and flowers,and are nutritionally valuable components of the human diet.The DFR gene plays an important role in the anthocy...Anthocyanins are secondary metabolites in land plants that contribute to the colors of leaves and flowers,and are nutritionally valuable components of the human diet.The DFR gene plays an important role in the anthocyanin biosynthetic pathway.In this study,we investigated the regulation of DFR expression and in different Malus crabapple cultivars that show distinct patterns of leaf coloration,and how it influences leaf anthocyanin accumulation and coloration.Specifically,we studied the ever-red leaved cultivar‘Royalty’,the ever-green leaved cultivar‘Flame’and the spring-red leaved cultivar‘Radiant’.RT-PCR analysis showed that the expression of McDFR1 correlated with the expression of a MYB transcription factor,McMYB10,and with anthocyanin accumulation.We isolated five McDFR1 promoter fragments from the three cultivars and identified four different fragments(F1–4)that were present either in several cultivars,or only in one.Yeast one-hybrid and electrophoretic mobility shift assay analyses showed that McMYB10 could bind to all the McDFR1 promoters,except McDFR1-Ra2.The F1,F2 and F3 fragments did not affect McMYB10 binding to the McDFR1 promoters;however,we found evidence that the F4 fragment suppressed binding,and that the MYBGAHV amino-acid sequence maybe an important cis-element for McMYB10 protein binding.This information has potential value for strategies to modify plant color through genetic transformation.展开更多
基金Financial support was provided by the‘National Natural Science Foundation of China’(31501723)‘The Importation and Development of High-Caliber Talents Project of Beijing Municipal Institutions’,the Beijing Technology Innovation Service Capacity Construction-Research Plan(KM201610020003)+1 种基金Scientific research improvement project of BUA(GL2015002)‘the Project of Construction of Innovative Teams and the Teacher Career Development for Universities and Colleges Under Beijing Municipality’(IDHT20150503).
文摘Anthocyanins are secondary metabolites in land plants that contribute to the colors of leaves and flowers,and are nutritionally valuable components of the human diet.The DFR gene plays an important role in the anthocyanin biosynthetic pathway.In this study,we investigated the regulation of DFR expression and in different Malus crabapple cultivars that show distinct patterns of leaf coloration,and how it influences leaf anthocyanin accumulation and coloration.Specifically,we studied the ever-red leaved cultivar‘Royalty’,the ever-green leaved cultivar‘Flame’and the spring-red leaved cultivar‘Radiant’.RT-PCR analysis showed that the expression of McDFR1 correlated with the expression of a MYB transcription factor,McMYB10,and with anthocyanin accumulation.We isolated five McDFR1 promoter fragments from the three cultivars and identified four different fragments(F1–4)that were present either in several cultivars,or only in one.Yeast one-hybrid and electrophoretic mobility shift assay analyses showed that McMYB10 could bind to all the McDFR1 promoters,except McDFR1-Ra2.The F1,F2 and F3 fragments did not affect McMYB10 binding to the McDFR1 promoters;however,we found evidence that the F4 fragment suppressed binding,and that the MYBGAHV amino-acid sequence maybe an important cis-element for McMYB10 protein binding.This information has potential value for strategies to modify plant color through genetic transformation.
