BACKGROUND Pyroptosis impacts the development of malignant tumors,yet its role in colorectal cancer(CRC)prognosis remains uncertain.AIM To assess the prognostic significance of pyroptosis-related genes and their assoc...BACKGROUND Pyroptosis impacts the development of malignant tumors,yet its role in colorectal cancer(CRC)prognosis remains uncertain.AIM To assess the prognostic significance of pyroptosis-related genes and their association with CRC immune infiltration.METHODS Gene expression data were obtained from The Cancer Genome Atlas(TCGA)and single-cell RNA sequencing dataset GSE178341 from the Gene Expression Omnibus(GEO).Pyroptosis-related gene expression in cell clusters was analyzed,and enrichment analysis was conducted.A pyroptosis-related risk model was developed using the LASSO regression algorithm,with prediction accuracy assessed through K-M and receiver operating characteristic analyses.A nomo-gram predicting survival was created,and the correlation between the risk model and immune infiltration was analyzed using CIBERSORTx calculations.Finally,the differential expression of the 8 prognostic genes between CRC and normal samples was verified by analyzing TCGA-COADREAD data from the UCSC database.RESULTS An effective pyroptosis-related risk model was constructed using 8 genes-CHMP2B,SDHB,BST2,UBE2D2,GJA1,AIM2,PDCD6IP,and SEZ6L2(P<0.05).Seven of these genes exhibited differential expression between CRC and normal samples based on TCGA database analysis(P<0.05).Patients with higher risk scores demonstrated increased death risk and reduced overall survival(P<0.05).Significant differences in immune infiltration were observed between low-and high-risk groups,correlating with pyroptosis-related gene expression.CONCLUSION We developed a pyroptosis-related prognostic model for CRC,affirming its correlation with immune infiltration.This model may prove useful for CRC prognostic evaluation.展开更多
This paper proposes new methods and strategies for Multi-UAVs cooperative attacks with safety and time constraints in a complex environment.Delaunay triangle is designed to construct a map of the complex flight enviro...This paper proposes new methods and strategies for Multi-UAVs cooperative attacks with safety and time constraints in a complex environment.Delaunay triangle is designed to construct a map of the complex flight environment for aerial vehicles.Delaunay-Map,Safe Flight Corridor(SFC),and Relative Safe Flight Corridor(RSFC)are applied to ensure each UAV flight trajectory's safety.By using such techniques,it is possible to avoid the collision with obstacles and collision between UAVs.Bezier-curve is further developed to ensure that multi-UAVs can simultaneously reach the target at the specified time,and the trajectory is within the flight corridor.The trajectory tracking controller is also designed based on model predictive control to track the planned trajectory accurately.The simulation and experiment results are presented to verifying developed strategies of Multi-UAV cooperative attacks.展开更多
Nitrogen huff-n-puff(N_(2)HnP) appears to be an economical and high-efficiency enhanced oil recovery(EOR) technique for tight oil reservoirs.There is however a lack of understanding of the pore-level EOR performance o...Nitrogen huff-n-puff(N_(2)HnP) appears to be an economical and high-efficiency enhanced oil recovery(EOR) technique for tight oil reservoirs.There is however a lack of understanding of the pore-level EOR performance of N2HnP under tight reservoir conditions.In this work,a non-magnetic reactor was created and combined with a nuclear magnetic resonance(NMR) device for real-time monitoring of oil distribution in the HnP experiment.N_(2)HnP experiments were then performed in a tight sandstone core sample at a temperature of 353 K and an injection pressure≥ 24 MPa.The pore-level oil distribution under reservoir conditions was monitored and the EOR performance of N2HnP in specific pores was analyzed.The pore throat structures of the core sample and the phase behavior of the N_(2)-Oil system were analyzed to elucidate the EOR mechanism of N_(2)HnP.An oil recovery factor of 37.52% can be achieved after four cycles,which proves the EOR potential of N_(2)HnP for tight reservoirs.The highest recoveries after N_(2)HnP are obtained in the large pores,followed by the medium pores,the small pores,and finally the micro pores.Increases in soaking time and injection pressure resulted in slight and pronounced increases in oil recovery,respectively,both of which are mainly reflected in the first cycle.Specifically,increasing the soaking time only slightly improves the cumulative oil recovery in the small pores while increasing the injection pressure significantly improves the cumulative oil recovery in the small,medium,and large pores simultaneously.However,variations in both injection pressure and soaking time have a negligible effect on the cumulative oil recovery of the micro pores.展开更多
Herein,the degradation of florfenicol(FLO)over zero-valent iron(ZVI)enhanced by SiC was systematically investigated.It was found that 5 g/L of ZVI/SiC(1:3)at pH 3.0 could completely degrade 20 mg/L of FLO within 1 h,w...Herein,the degradation of florfenicol(FLO)over zero-valent iron(ZVI)enhanced by SiC was systematically investigated.It was found that 5 g/L of ZVI/SiC(1:3)at pH 3.0 could completely degrade 20 mg/L of FLO within 1 h,with a Kobsvalue of 0.0873 min^(-1),12.5 times greater than that of pure ZVI(0.0069 min^(-1)).Vibrating sample magnetometer(VSM)characterizations revealed that the use of SiC supporter reduces the magnetic intensity of ZVI,which mitigates iron particle agglomeration,increases Brunauer-Emmett-Teller(BET)surface area,and enhances FLO degradation efficiency.Furthermore,ZVI/SiC exhibits a much lower hydrogen evolution potential(HEP)and significantly higher corrosion currents compared to pure ZVI.FLO was proposed to undergo degradation via reductive dechlorination,involving a hydrogenolysis mechanism that entails the cleavage of theσbond.This study provides new insights into the reduction hydrogenation mechanism of ZVI.展开更多
Noise generated by trains running on elevated lines creates many disturbances to the normal lives of surrounding residents. Investigations have shown that people living along elevated lines complain that the noise is ...Noise generated by trains running on elevated lines creates many disturbances to the normal lives of surrounding residents. Investigations have shown that people living along elevated lines complain that the noise is sometimes unbearable. To better control the noise and optimize the acoustic environment, noise spectrum characteristics were analyzed and compared with a field test and a numerical simulation. Through an energy analysis of the noise on the bridge side, the energy distribution characteristics of the noise at specific measuring points in different frequency bands were obtained. The influence of the Doppler effect on frequency shift was analyzed. Based on the partial coherence theory, a multi-input and single-output program was compiled to calculate the correlation and contribution degree of the bridge structure-borne noise and wheel/rail noise at the one-third octave center frequency. The results show that the peak noises of the bridge and the wheel/rail are concentrated at 31.5–63 Hz and 400–800 Hz, respectively. For environmental noise on the bridge side, the frequency band above 250 Hz is mainly affected by the wheel/rail noise. In areas of noise source strength, the relative ratio of noise energy above 250 Hz can reach 83.4%. Noise in the near ground and far bridge area is mainly low-frequency, and the relative energy ratio is about 8.9%. The Doppler effect has an influence of less than 6% on the frequency shift with a speed of 67.9 km/h. In the low-frequency band below 250 Hz, the noise in the acoustic shadow area near the bridge and the ground is mainly contributed to by the vibration-radiated noise of the bridge, of which the contribution of the bottom panel is the most prominent. The noise in the comprehensive noise area of the far bridge is mainly caused by the structure-borne noise of the bridge, and the contribution of each bridge panel is different. This study can provide a reference for finding the source of elevated rail noise in some challenging frequency ranges and for then determining optimal designs and measures for noise reduction.展开更多
Folate-containing dextran ligand （FA-Dextran-DTPA） was synthesized by the incorporation of diethylenetriamine- pentaacetic acid （DTPA） and folate （FA） as a tumor-targeting group into dextran as a polymer carrier...Folate-containing dextran ligand （FA-Dextran-DTPA） was synthesized by the incorporation of diethylenetriamine- pentaacetic acid （DTPA） and folate （FA） as a tumor-targeting group into dextran as a polymer carrier. This ligand was further reacted with gadolinium chloride to make a dextran gadolinium complex FA-Dextran-DTPA-Gd. The ligand and its gadolinium complex were characterized by 1H-NMR, FTIR, UV-Vis, average particle sizes and zeta potential, as well. In vitro properties including relaxivity, cytotoxicity assay, cellular uptake assay, and magnetic resonance imaging （MRI） were also evaluated. Compared with Gd-DTPA, FA-Dextran-DTPA-Gd possessed obviously higher relaxation effectiveness and lower cytotoxicity to HeLa cells. FA-Dextran-DTPA-Gd had a high affinity to the H460 and MDA-MB-231 tumor cells and can be taken up selectively by these tumor cells. Moreover, FA-Dextran-DTPA-Gd showed enhanced signal intensities （SI） of MRI and enhanced the contrast of MR images of tumor cells. These results indicated that FA-Dextran-DTPA-Gd showed the potential as a tumor-targeting contrast agent in MRI.展开更多
基金Supported by the National Natural Science Foundation of China,No.81960100Applied Basic Foundation of Yunnan Province,No.202001AY070001-192+2 种基金Young and Middle-aged Academic and Technical Leaders Reserve Talents Program in Yunnan Province,No.202305AC160018Yunnan Revitalization Talent Support Program,No.RLQB20200004 and No.RLMY20220013and Yunnan Health Training Project of High-Level Talents,No.H-2017002。
文摘BACKGROUND Pyroptosis impacts the development of malignant tumors,yet its role in colorectal cancer(CRC)prognosis remains uncertain.AIM To assess the prognostic significance of pyroptosis-related genes and their association with CRC immune infiltration.METHODS Gene expression data were obtained from The Cancer Genome Atlas(TCGA)and single-cell RNA sequencing dataset GSE178341 from the Gene Expression Omnibus(GEO).Pyroptosis-related gene expression in cell clusters was analyzed,and enrichment analysis was conducted.A pyroptosis-related risk model was developed using the LASSO regression algorithm,with prediction accuracy assessed through K-M and receiver operating characteristic analyses.A nomo-gram predicting survival was created,and the correlation between the risk model and immune infiltration was analyzed using CIBERSORTx calculations.Finally,the differential expression of the 8 prognostic genes between CRC and normal samples was verified by analyzing TCGA-COADREAD data from the UCSC database.RESULTS An effective pyroptosis-related risk model was constructed using 8 genes-CHMP2B,SDHB,BST2,UBE2D2,GJA1,AIM2,PDCD6IP,and SEZ6L2(P<0.05).Seven of these genes exhibited differential expression between CRC and normal samples based on TCGA database analysis(P<0.05).Patients with higher risk scores demonstrated increased death risk and reduced overall survival(P<0.05).Significant differences in immune infiltration were observed between low-and high-risk groups,correlating with pyroptosis-related gene expression.CONCLUSION We developed a pyroptosis-related prognostic model for CRC,affirming its correlation with immune infiltration.This model may prove useful for CRC prognostic evaluation.
基金National Natural Science Foundation of China(No.61903350)Beijing Institute of Technology Research Fund Program for Young Scholars。
文摘This paper proposes new methods and strategies for Multi-UAVs cooperative attacks with safety and time constraints in a complex environment.Delaunay triangle is designed to construct a map of the complex flight environment for aerial vehicles.Delaunay-Map,Safe Flight Corridor(SFC),and Relative Safe Flight Corridor(RSFC)are applied to ensure each UAV flight trajectory's safety.By using such techniques,it is possible to avoid the collision with obstacles and collision between UAVs.Bezier-curve is further developed to ensure that multi-UAVs can simultaneously reach the target at the specified time,and the trajectory is within the flight corridor.The trajectory tracking controller is also designed based on model predictive control to track the planned trajectory accurately.The simulation and experiment results are presented to verifying developed strategies of Multi-UAV cooperative attacks.
基金financial support from the National Natural Science Foundation of China(52074319)the Strategic Cooperation Technology Project of CNPC(ZLZX2020-01-08)the Science Foundation of China University of Petroleum-Beijing(2462021QNXZ008)
文摘Nitrogen huff-n-puff(N_(2)HnP) appears to be an economical and high-efficiency enhanced oil recovery(EOR) technique for tight oil reservoirs.There is however a lack of understanding of the pore-level EOR performance of N2HnP under tight reservoir conditions.In this work,a non-magnetic reactor was created and combined with a nuclear magnetic resonance(NMR) device for real-time monitoring of oil distribution in the HnP experiment.N_(2)HnP experiments were then performed in a tight sandstone core sample at a temperature of 353 K and an injection pressure≥ 24 MPa.The pore-level oil distribution under reservoir conditions was monitored and the EOR performance of N2HnP in specific pores was analyzed.The pore throat structures of the core sample and the phase behavior of the N_(2)-Oil system were analyzed to elucidate the EOR mechanism of N_(2)HnP.An oil recovery factor of 37.52% can be achieved after four cycles,which proves the EOR potential of N_(2)HnP for tight reservoirs.The highest recoveries after N_(2)HnP are obtained in the large pores,followed by the medium pores,the small pores,and finally the micro pores.Increases in soaking time and injection pressure resulted in slight and pronounced increases in oil recovery,respectively,both of which are mainly reflected in the first cycle.Specifically,increasing the soaking time only slightly improves the cumulative oil recovery in the small pores while increasing the injection pressure significantly improves the cumulative oil recovery in the small,medium,and large pores simultaneously.However,variations in both injection pressure and soaking time have a negligible effect on the cumulative oil recovery of the micro pores.
基金financially supported by the Guangdong Basic and Applied Basic Research Foundation(No.2021A1515110954)National Key Research and Development Projects of China(No.2022YEC3203302)+1 种基金Guangdong Province Enterprise Science and Technology Commissioner Project(No.GDKTP2021048000)National Natural Science Foundation of China(No.41907292)。
文摘Herein,the degradation of florfenicol(FLO)over zero-valent iron(ZVI)enhanced by SiC was systematically investigated.It was found that 5 g/L of ZVI/SiC(1:3)at pH 3.0 could completely degrade 20 mg/L of FLO within 1 h,with a Kobsvalue of 0.0873 min^(-1),12.5 times greater than that of pure ZVI(0.0069 min^(-1)).Vibrating sample magnetometer(VSM)characterizations revealed that the use of SiC supporter reduces the magnetic intensity of ZVI,which mitigates iron particle agglomeration,increases Brunauer-Emmett-Teller(BET)surface area,and enhances FLO degradation efficiency.Furthermore,ZVI/SiC exhibits a much lower hydrogen evolution potential(HEP)and significantly higher corrosion currents compared to pure ZVI.FLO was proposed to undergo degradation via reductive dechlorination,involving a hydrogenolysis mechanism that entails the cleavage of theσbond.This study provides new insights into the reduction hydrogenation mechanism of ZVI.
基金Project supported by the National Natural Science Foundation of China (Nos. 5140843411772230and 51678446)。
文摘Noise generated by trains running on elevated lines creates many disturbances to the normal lives of surrounding residents. Investigations have shown that people living along elevated lines complain that the noise is sometimes unbearable. To better control the noise and optimize the acoustic environment, noise spectrum characteristics were analyzed and compared with a field test and a numerical simulation. Through an energy analysis of the noise on the bridge side, the energy distribution characteristics of the noise at specific measuring points in different frequency bands were obtained. The influence of the Doppler effect on frequency shift was analyzed. Based on the partial coherence theory, a multi-input and single-output program was compiled to calculate the correlation and contribution degree of the bridge structure-borne noise and wheel/rail noise at the one-third octave center frequency. The results show that the peak noises of the bridge and the wheel/rail are concentrated at 31.5–63 Hz and 400–800 Hz, respectively. For environmental noise on the bridge side, the frequency band above 250 Hz is mainly affected by the wheel/rail noise. In areas of noise source strength, the relative ratio of noise energy above 250 Hz can reach 83.4%. Noise in the near ground and far bridge area is mainly low-frequency, and the relative energy ratio is about 8.9%. The Doppler effect has an influence of less than 6% on the frequency shift with a speed of 67.9 km/h. In the low-frequency band below 250 Hz, the noise in the acoustic shadow area near the bridge and the ground is mainly contributed to by the vibration-radiated noise of the bridge, of which the contribution of the bottom panel is the most prominent. The noise in the comprehensive noise area of the far bridge is mainly caused by the structure-borne noise of the bridge, and the contribution of each bridge panel is different. This study can provide a reference for finding the source of elevated rail noise in some challenging frequency ranges and for then determining optimal designs and measures for noise reduction.
基金financially supported by the National Natural Science Foundation of China(Nos.51173140 and 51373128)Wuhan Scientific and Technological Project(No.2013010501010131)+1 种基金Wuhan Science and Technology Innovation Team of Hi-tech Industrial Project,Hubei Province(No.2015070504020217)Innovation Fund for Post-graduate Education(Nos.CX2014058 and CX2013083),Wuhan Institute of Technology,China
文摘Folate-containing dextran ligand （FA-Dextran-DTPA） was synthesized by the incorporation of diethylenetriamine- pentaacetic acid （DTPA） and folate （FA） as a tumor-targeting group into dextran as a polymer carrier. This ligand was further reacted with gadolinium chloride to make a dextran gadolinium complex FA-Dextran-DTPA-Gd. The ligand and its gadolinium complex were characterized by 1H-NMR, FTIR, UV-Vis, average particle sizes and zeta potential, as well. In vitro properties including relaxivity, cytotoxicity assay, cellular uptake assay, and magnetic resonance imaging （MRI） were also evaluated. Compared with Gd-DTPA, FA-Dextran-DTPA-Gd possessed obviously higher relaxation effectiveness and lower cytotoxicity to HeLa cells. FA-Dextran-DTPA-Gd had a high affinity to the H460 and MDA-MB-231 tumor cells and can be taken up selectively by these tumor cells. Moreover, FA-Dextran-DTPA-Gd showed enhanced signal intensities （SI） of MRI and enhanced the contrast of MR images of tumor cells. These results indicated that FA-Dextran-DTPA-Gd showed the potential as a tumor-targeting contrast agent in MRI.
