MicroRNA-184 promotes proliferation ability of glioma cells by regulating FOXO3

Objective:To investigate the effect of microRNA(miR-184)on regulating the genesis.development and prolifration of glioma cells.Methods:Lipidosome was used to transfect miR-184 mimic and inhibitor to glioma cell ling,and the cell proliferation ability changes were determined by MTT and plate cloning experiment after the transfection.WB test was used to measure the levels of cyclinD1,p27 and FOXO3.Meanwhile.QPCR was used to detect miR-184expression in glioma cell line.glioma tissues and adjacent tissues.Luciferase experiment was used to test 3'UTR gene targeting regulation of miR-184 and FOXO3.Results:QPCR results showed a significaat lower miR-184 expression level in glioma cell line and glioma tissues than that in juxtacancerous tissue.MTT and plate cloning experiments have shown that after overexpressing of miR-184,the cell proliferation capacity of glioma U87 and T98G was signifieantly increased,which was significantly inhibited after the inhibition of miN-184.WB results showed a lower expression level of p27 in U87 and T98G cells,and a higher expression level of cyclind1after over-expressing of miR-184 wss observed.However.a lower expression level of eyelinD1and a higher expression level of p27 after the inhibition of miR-184.The luciferase activity was inhibited after the over-expressing of miR-184.Conclusions:MiR-184 can affect the proliferation abilities of glioma cells and regulate the cell cycle related protein.It plays an important role in the occurrence and development of gliomas.

Cucurbitane triterpenoids from Hemsleya penxianensis

Two new cucurbitacins,jinfushanencins A(1)and B(2),seven new cucurbitane glycosides,jinfushanosides E-K(3-9),along with nine known analogues,were obtained from the tubers of Hemsleya penxianensis.Their structures were elucidated on the basis of extensive spectroscopic and chemical methods.Selected isolates were tested their anti-HIV-1 activities,and compound 5 showed weak anti-HIV-1 in C8166 cell(EC50=5.9μg/mL)with a selectivity index of 13.5.

Fujita Type Conditions to Heat Equation with Variable Source

This paper studies heat equation with variable exponent ut = △u ＋ Up（x） 4- Uq in RN × （0, T）, where p（x） is a nonnegative continuous, bounded function, 0 〈 p- = infp（x） ≤ p（x） ≤ supp（x） = p＋. It is easy to understand for the problem that all nontrivial nonnegative solutions must be global if and only if max{p＋,q} ≤1. Based on the interaction between the two sources with fixed and variable exponents in the model, some Fujita type conditions are determined that that all nontrivial nonnegative solutions blow up in finite time if 0 〈 q ≤ 1 with p＋ 〉 1, or 1 〈 q 〈 1 ＋2/N. In addition, if q 〉 1 ＋2/N, then （i） all solutions blow up in finite time with 0 〈 p- ≤ p＋ ≤ 1 ＋2/N; （ii） there are both global and nonglobal solutions for p- ≤ 1 ＋ 2/N; and （iii） there are functions p（x） such that all solutions blow up in finite time, and also functions p（x） such that the problem possesses global solutions when p＋〈 1＋2/N 〈 p＋.

Dermatopathic Lymphadenitis

To the Editor： Dermatopathic lymphadenitis （DL） represents a rare benign lymphatic hyperplasia commonly associated with exfoliative or eczematoid dermatitis. DL was also reported to be a potential mimicker of lymphoma. Here, we report the ^18F-fluorodeoxyglucose （^18F-FDG） positron emission tomography/ computer tomograpby （PET/CT） results and the histological findings of a 40-year-old female patient with DL.

18F-fluorodeoxyglucose Uptake with Expression of Excision Repair Cross-complementary Group I and Ribonucleotide Reductase Subunit M1 in Non-small Cell Lung Cancer

Fluorodeoxyglucose positron emission tomography/conlputed tomography （FDG PET/CT） is widely applied in non-small cell lung cancer （NSCLC）. The standardized uptake value （SUV）, a semi-quantitative index, plays an essential role in NSCLC tbr diagnosis, staging, and efficacy evaklation. It has been px3posed that the SUV of tumors may correlate with the presence or absence of chemotherapy resistance-associated biomarkers based on studies that have displayed a close correlation between SUV and the expression levels of excision repair cross-complementary Group 1 （ERCC 1 ）1~1 and Tp53-induced glycolysis and apoptosis regulator.121 FDG avidity of NSCLC and ERCC 1 and ribonucleotide reductase subunit M 1 （RRM 1 ） levels have not been as extensively investigated. Based on these findings, we looked tbr correlations among metabolic parameters （SUVm,,. metabolic tumor volume [MTV], and total lesion glycolysis [TLG]） and ERCC1 and RRM1 expression in patients with NSCLC, to investigate whether FDG uptake reflects the presence or absence ofchemoresistance proteins （ERCC1 and RRM 1 ） within tumor cells.