To the Editor:Ankle sprains are some of the most common emergency injuries from trauma.Due to the particularity of its anatomy,most sprains of the ankle are due to injuries of the lateral collateral ligaments,of which...To the Editor:Ankle sprains are some of the most common emergency injuries from trauma.Due to the particularity of its anatomy,most sprains of the ankle are due to injuries of the lateral collateral ligaments,of which 80%are tears or ruptures of the anterior talofibular ligament(ATFL).[1]If the treatment is not appropriate,the injury will affect the patient’s work and life in the long term,and the patient can even develop chronic ankle instability(CAI),increasing the treatment duration and resulting inhigher economic burdens on the patients and themedical insurance system.Regarding the clinical epidemiology of ATFL injuries,at present,most research is focused on the exploration of the incidence,pathogenic causes,and treatment methods,but not enough attention has been given to individualized factors and the associated injuries.In addition,the guidance effect of the findings of these studies on clinical practice is limited.To the best of our knowledge,there have been no clinical epidemiological studies on ATFL injuries in ward patients in China.Therefore,this study retrospectively analyzed the clinical data of all patients with ATFL injuries in the Sports Medicine Center of the Southwest Hospital ofArmy Military Medical University from July 2007 to December 2020.The goal was to understand the characteristics and pattern distributions of ATFL injuries to provide a basis for their prevention,early diagnosis,and treatment.This study was approved by the Ethics Committee of The First Affiliated Hospital of Army Medical University(KY 2021026).展开更多
Carbonic anhydrase accounts for catalytic reaction of CO_(2)/HCO_(3)^(–) transformation, thus resulting in neutralization and acidification of the cellular environment, thereby favoring tumor development. Hence, it i...Carbonic anhydrase accounts for catalytic reaction of CO_(2)/HCO_(3)^(–) transformation, thus resulting in neutralization and acidification of the cellular environment, thereby favoring tumor development. Hence, it is a classical protein model of greatly biocatalytic significance as well as a highly expressed biomarker with renal tumor. We herein proposed a single-molecule measurement on carbonic anhydrase using MspA nanopore, in [BMIM+] and asymmetric K^(+)/Ca^(2+) cationic coordinated environment, instead of usual symmetric KCl/NaCl electrolyte. Significantly, our empirical analysis showed that asymmetric K^(+)/Ca^(2+) cationic environment contributes to distinguishable current modulations, thus yielding better resolution for carbonic anhydrase measurement, which is independent of applied voltage and more importantly, is stable enough at varied pH conditions and for very low concentration test in urine sample. Our results provide a classical model for nanopore protein analysis, and may also permit biocatalytic measurement at single-molecule level.展开更多
基金National Natural Science Foundation of China(No.82072516)
文摘To the Editor:Ankle sprains are some of the most common emergency injuries from trauma.Due to the particularity of its anatomy,most sprains of the ankle are due to injuries of the lateral collateral ligaments,of which 80%are tears or ruptures of the anterior talofibular ligament(ATFL).[1]If the treatment is not appropriate,the injury will affect the patient’s work and life in the long term,and the patient can even develop chronic ankle instability(CAI),increasing the treatment duration and resulting inhigher economic burdens on the patients and themedical insurance system.Regarding the clinical epidemiology of ATFL injuries,at present,most research is focused on the exploration of the incidence,pathogenic causes,and treatment methods,but not enough attention has been given to individualized factors and the associated injuries.In addition,the guidance effect of the findings of these studies on clinical practice is limited.To the best of our knowledge,there have been no clinical epidemiological studies on ATFL injuries in ward patients in China.Therefore,this study retrospectively analyzed the clinical data of all patients with ATFL injuries in the Sports Medicine Center of the Southwest Hospital ofArmy Military Medical University from July 2007 to December 2020.The goal was to understand the characteristics and pattern distributions of ATFL injuries to provide a basis for their prevention,early diagnosis,and treatment.This study was approved by the Ethics Committee of The First Affiliated Hospital of Army Medical University(KY 2021026).
基金National Key Research and Development Program of China(2022YFB3205600)Chongqing Talents:Exceptional Young Talents Project(cstc2021ycjh-bgzxm0016)+1 种基金Beibei Technology Talents and Independent Innovation Project(No.2022-34)the Natural Science Foundation of Chongqing,China(cstc2021jcyj-jqx0030).
文摘Carbonic anhydrase accounts for catalytic reaction of CO_(2)/HCO_(3)^(–) transformation, thus resulting in neutralization and acidification of the cellular environment, thereby favoring tumor development. Hence, it is a classical protein model of greatly biocatalytic significance as well as a highly expressed biomarker with renal tumor. We herein proposed a single-molecule measurement on carbonic anhydrase using MspA nanopore, in [BMIM+] and asymmetric K^(+)/Ca^(2+) cationic coordinated environment, instead of usual symmetric KCl/NaCl electrolyte. Significantly, our empirical analysis showed that asymmetric K^(+)/Ca^(2+) cationic environment contributes to distinguishable current modulations, thus yielding better resolution for carbonic anhydrase measurement, which is independent of applied voltage and more importantly, is stable enough at varied pH conditions and for very low concentration test in urine sample. Our results provide a classical model for nanopore protein analysis, and may also permit biocatalytic measurement at single-molecule level.
