Network analysis of suicide ideation and depression-anxiety symptoms among Chinese adolescents

Background The co-occurrence of depression and anxiety among adolescents is typically associated with suicide ideation.Aims The study aimed to investigate the symptom-level relationship between suicide ideation and the comorbidity of depression and anxiety.Methods 1501 adolescents aged 12-19 years were assessed using the Patient Health Questionnaire(PHQ-9)and the Generalized Anxiety Disorder Scale,and 716 adolescents who scored≥5 on both scales were selected as participants.Network analysis was used to identify the network structure of depressive symptoms and anxiety symptoms.Participants were categorised into either the suicide ideation or non-suicide ideation groups based on their scoring on the suicide-related item in PHQ-9.A comparison was made between the depression-anxiety symptom networks of the two groups.Results‘Restlessness’,‘sad mood’and‘trouble relaxing’were the most prominent central symptoms in the depression-anxiety symptom network,and‘restlessness’,‘nervousness’and‘reduced movement’were the bridge symptoms in this network.‘Sad mood’was found to be directly related to‘suicide ideation’with the highest variance.The network structure was significantly different in properties between the suicide ideation group and the non-suicide ideation group,with‘restlessness’and‘sad mood’exhibiting significantly higher influence in the network of the suicide ideation group than that in the non-suicide ideation group.Conclusion Restlessness and sad mood could be targeted for the intervention of depression-anxiety symptoms among adolescents with suicide ideation.

Nonlinear Refraction of Peripheral-Substituted Zinc Phthalocyanines Investigated by Nanosecond and Picose-cond Z Scans

The singlet and triplet excited-state refraction cross-sections of dimethyl sulfoxide (DMSO) solutions of ten zinc phthalocyanine derivatives with mono-or tetra-peripheral substituents at 532 nm were obtained by simultaneous fitting of closed-aperture Z scans with both nanosecond and picosecond pulse widths. Self-focusing of both nanosecond and picosecond laser pulses was observed in all complexes at 532-nm wavelength. The complexes with tetra-substituents at the ?-position exhibit relatively larger refraction cross-sections than the other complexes. The wavelength dependence of the singlet refraction cross-section of a representative complex was observed to be non-monotonic in the range of 470 - 550 nm.

体表经纬穿刺定位法在胸腔镜肺磨玻璃结节手术中的初步应用

背景与目的肺磨玻璃结节在胸腔镜手术中如何定位是微创胸外科的重要临床课题,目前尚无统一的定位方法。本研究拟探讨体表经纬法穿刺定位法在电视胸腔镜肺磨玻璃结节手术中肺结节定位的准确性及安全性。方法回顾性分析2018年8月-2019年12月期间我院收治的41例肺磨玻璃结节患者的临床资料,其中男性28例,女13例。患者于麻醉后采用体表经纬法穿刺法定位,然后行胸腔镜下部分肺叶切除术。对手术切除标本测量结节至标记缝线距离和结节至切缘的距离,统计定位准确率、并发症率和手术切除成功率。结果采用体表经纬法穿刺定位法对41例患者共51枚结节进行定位,准确率达96.1%,平均定位时间8.3 min。有5例(12.2%)出现穿刺出血,均胸腔镜下成功止血,无其他并发症出现。所有患者行胸腔镜部分肺叶切除术,其中33例行解剖性肺段切除,8例行肺叶楔形切除术,手术过程均顺利。测量结节与切缘的距离,所有标本均>2 cm,达到安全距离,手术切除成功率100.0%。结论在电视胸腔镜肺磨玻璃结节手术中,体表经纬穿刺定位法是准确、安全、简便的定位方法。

Copy number and zygosity determination of transgenic rapeseed by droplet digital PCR

Establishing an accurate and rapid method for copy number and zygosity determination can accelerate genetic engineering research process. In this study, droplet digital PCR (ddPCR), an emerging DNA absolute quantification technology, was used to identify single-copy homozygous transgenic lines from a batch of T0 transgenic rapeseed harboring 11 exogenous elements. Copy number of exogenous gene was evaluated in T0 generation based on calculated ratio between transgene and reference CruA gene, single-copy transformants were selected for selfing followed by subsequent zygosity analysis. Single-copy homozygous transgenic plants were successfully screened out in T1 generation by ddPCR.Segregation analysis with T2 seedlings verified that identification results of ddPCR were accurate and reliable. This study provides a novel rapid and accurate method for copy number and zygosity determination in transgenic rapeseed which overcomes disadvantages of traditional Southern analysis and recently developed real-time quantitative method.

An efficient method to extract DNA from refined rapeseed oil

Genetically modified oilseeds were used as processing raw material for edible oils. The protection of consumer rights to information as well as the genetically modified orgamisms （GMO） labe-ling polily required analytical methods to determine whether the oils contained genetically modified ingre-dient or not. Polymerase chain reaction （PCR） - based method was used commonly to determine the presence of GMOs. Adulteration attracted peoples concern also. Thus it was crucial that enough DNA ex-tracts can be obtained successfully from oil samples. For the purpose, three DNA extraction methods （modified emulsification method, the kits Wizard Magnetic DNA purification system for food and Nucleospin Food） ,were applied to 3 different grades of rapeseed oil samples. Those methods were compared by the amplification of Brassica napus reference gene CruA using real - time PCR. The results demonstra-ted that both the modified emulsification and the Nucleospin methods were able to extract enough DNA from refined oils. The modified emulsification method was superior to the kit of Nucleospin food due to smaller volume of required sample.

Rapid screening of genetically modified ingredients in soybean and cotton processing by-product and waste using direct qPCR

To develop a simple and fast method for screening genetically modified ingredients from processing by-product and waste,direct quantitative PCR(qPCR)kit-Taqman which omitting multi genomic DNA preparing steps was developed in this study.A total of 18 oil crop processing by-products and wastes including 10 soybean and 8 cotton materials were collected from food processing factories.Compared with 2 commercial direct qPCR kits,conditions of DNA releasing procedure and PCR amplification were optimized.Element screening was performed at the initial step of genetically modified(GM)ingredient testing procedure via direct qPCR.GM event identification was carried out in positive samples by initial screening.Totally 5 screening elements(P–35S,T-NOS,Cp4-epsps,bar and pat)for soybean materials and 6 screening elements(P–35S,T-NOS,NPTII,Cry1Ac,bar and pat)for cotton samples were detected.In GM event identification,MON531 and MON1445 were found in cotton materials.Results were further confirmed by real-time PCR with DNA extraction and purification.The direct qPCR system proposed by this research was convenient for rapid screening and identification of GM ingredients in oil crop primary by-product and waste.

Effects of non-procedural factors in Brassica napus genetic transformation

Rapeseed (Brassica napus) is the second largest oil crop in the world. However, transformation efficiency of rapeseed still needs to be improved. To evaluate non-procedural factors (e.g. explants, section of explant, marker genes and number of exogenous genes) effects on transformation efficiency, 6-day-old hypocotyl explants from in vitro grown seedlings were co-cultivated with Agrobacterium strain GV3101 harboring a binary vector using optimized transformation procedure. Results showed that normal maturing variety ‘Zhongshuang 6 (ZS6)’ had the highest overall capacity to produce rooted shoots among 5 common varieties and 6 early maturing varieties, with green callus induction rate 81.45% and shoot regeneration rate 21.66%. Early maturing variety 14M645 has relatively high regeneration rate (4.69%) and one of the shortest growth periods (107.54 d). Data showed that choosing neomycin phosphotransferase II gene (NPTII) as selectable marker led to the best transformation rate (17.38%). Selecting upper hypocotyl segments near cotyledon as explant provided the higest transformation efficiency, with regeneration rate of 25.59% when using NPTII as selectable marker and 22.19% for Bar. B. napus transformed with single gene showed higher transformation frequency than vectors with multiple genes,highlighting difficulty of multiple gene transformation. This work helped to further improve genetic transformation of B. napus by optimizing factors that impact transformation efficiency,and it would ultimately improve research in transgenic B. napus varieties with commercial potential.

Evaluation of five DNA extraction methods for commercial vegetable oils

To ensure food safety, it's vital to accurately detect genetically modified(GM)ingredient adulteration in food products and effectively detect the adulteration of vegetable oils from GM organisms(GMO). Therefore, it's essential to establish efficient DNA isolation method from vegetable oil. Here, we evaluate 5 DNA isolation methods using 25 commercial vegetable oils produced from soybean, peanut, corn, sunflower, rapeseed as well as blended oils. Quality of isolated DNA was determined by Nanodrop 2000 spectrophotometry. Real-time PCR and universal gene tRNA-Leu was used to assess resolution of methods. Our results showed that only DNA samples isolated by modified emulsification method based on cetyl trimethylammonium bromide(CTAB) were able to amplify t RNA-Leu gene.Moreover, Ct values of species specific endogenous reference genes were greater than 36 in these samples. In summary, CTAB method showed the best resolution on GMO adulteration detection for commercial vegetable oils, especially in fully refined oils.