Purpose:To investigate the effect of NF-κB inhibitor PDTC on inflammation response in mice with herpetic stromal keratitis (HSK). Methods:A total of 120 female BALB/c mice,aged 4 -6 weeks, were treated by scuffing th...Purpose:To investigate the effect of NF-κB inhibitor PDTC on inflammation response in mice with herpetic stromal keratitis (HSK). Methods:A total of 120 female BALB/c mice,aged 4 -6 weeks, were treated by scuffing the epithelium of the right cornea with the tip of a 30G syringe needle, and approximately 1×106 PFU of HSV-1virus was seeded onto the corneal surface. The eye was then exposed to 170 mJ/cm2 of UV light 7 weeks later, in order to induce the relapse of HSK. PDTC eye drops were adjusted to various concentrations (0.1,1.0, and 10 mg/ml) with PBS; PBS only was used on control animals.All animals received PDTC or PBS eye drops 6 times/day,1 drop/time, for 7 consecutive days. Mice were sacrificed at 8h, and 1, 3, and 7d after administration. Corneal tissues were prepared for the detection of IL-1β, IL-4, IL-6, and IL-12 by ELISA and histological study. Results: Neutrophilic leukocytes and lymphocytes were found in the corneas of HSK mice. ELISA results showed fluctuating expressions of IL-1β, IL-4, IL-6, and IL-12. The IL-1β and IL-4 expressions were significantly lower in the high dose group than in the control group (P<0.05), while IL-6 and IL-12 expressions were significantly higher in the high dose group than in the control group (P<0.05). Conclusion:A high concentration of PDTC can suppress the expression of certain inflammatory factors in HSK mice while improving the expression of others.展开更多
基金supported by the Fund for Medical Sciences of Guangdong Province (A2009463)the Internal Fund of Shantou International Eye Center (10-002)
文摘Purpose:To investigate the effect of NF-κB inhibitor PDTC on inflammation response in mice with herpetic stromal keratitis (HSK). Methods:A total of 120 female BALB/c mice,aged 4 -6 weeks, were treated by scuffing the epithelium of the right cornea with the tip of a 30G syringe needle, and approximately 1×106 PFU of HSV-1virus was seeded onto the corneal surface. The eye was then exposed to 170 mJ/cm2 of UV light 7 weeks later, in order to induce the relapse of HSK. PDTC eye drops were adjusted to various concentrations (0.1,1.0, and 10 mg/ml) with PBS; PBS only was used on control animals.All animals received PDTC or PBS eye drops 6 times/day,1 drop/time, for 7 consecutive days. Mice were sacrificed at 8h, and 1, 3, and 7d after administration. Corneal tissues were prepared for the detection of IL-1β, IL-4, IL-6, and IL-12 by ELISA and histological study. Results: Neutrophilic leukocytes and lymphocytes were found in the corneas of HSK mice. ELISA results showed fluctuating expressions of IL-1β, IL-4, IL-6, and IL-12. The IL-1β and IL-4 expressions were significantly lower in the high dose group than in the control group (P<0.05), while IL-6 and IL-12 expressions were significantly higher in the high dose group than in the control group (P<0.05). Conclusion:A high concentration of PDTC can suppress the expression of certain inflammatory factors in HSK mice while improving the expression of others.
