The COVID-19 pandemic has emerged as a global health emergency due to its association with severe pneumonia and relative high mortality.However,the molecular characteristics and pathological features underlying COVID-...The COVID-19 pandemic has emerged as a global health emergency due to its association with severe pneumonia and relative high mortality.However,the molecular characteristics and pathological features underlying COVID-19 pneumonia remain largely unknown.To characterize molecular mechanisms underlying COVID-19 pathogenesis in the lung tissue using a proteomic approach,fresh lung tissues were obtained from newly deceased patients with COVID-19 pneumonia.After virus inactivation,a quantitative proteomic approach combined with bioinformatics analysis was used to detect proteomic changes in the SARS-CoV-2-infected lung tissues.We identified significant differentially expressed proteins involved in a variety of fundamental biological processes including cellular metabolism,blood coagulation,immune response,angiogenesis,and cell microenvironment regulation.Several inflammatory factors were upregulated,which was possibly caused by the activation of NF-κB signaling.Extensive dysregulation of the lung proteome in response to SARS-CoV-2 infection was discovered.Our results systematically outlined the molecular pathological features in terms of the lung response to SARS-CoV-2 infection,and provided the scientific basis for the therapeutic target that is urgently needed to control the COVID-19 pandemic.展开更多
Dear editor,Mycosis fungoides(MF),the most common subtype of cutaneous T cell lymphoma,is a rare disease[1,2].Patients with early-stageMF have a 5-year overall survival between 88%and 100%[3].In patients with advanced...Dear editor,Mycosis fungoides(MF),the most common subtype of cutaneous T cell lymphoma,is a rare disease[1,2].Patients with early-stageMF have a 5-year overall survival between 88%and 100%[3].In patients with advanced MF,the skin may present with tumors and erythroderma,and the median survival of patients with lymph node and visceral metastasis is 13 months[4].Therefore,it is important to achieve an early diagnosis to improve prognosis[5].展开更多
Dear Editor,Almost one-half of COVID-19 patients have symptoms of liver injury.However,the molecular characteristics in the COVID-19 liver remain unknown.Therefore,the need for long-term medication for recovery make i...Dear Editor,Almost one-half of COVID-19 patients have symptoms of liver injury.However,the molecular characteristics in the COVID-19 liver remain unknown.Therefore,the need for long-term medication for recovery make it imperative to study the infectivity and pathogenicity of SARS-CoV-2 in the liver.Here,we have identifed signifcant proteomic changes for the first time in the liver of COVID-19 patients.We detected differential expression levels of several proteins that mediate characteristic cellular host responses elicited by SARS-CoV-2 infection in the liver.展开更多
With the development of high-resolution and high-throughput mass spectrometry（MS）technology, a large quantum of proteomic data is continually being generated. Collecting and sharing these data are a challenge that r...With the development of high-resolution and high-throughput mass spectrometry（MS）technology, a large quantum of proteomic data is continually being generated. Collecting and sharing these data are a challenge that requires immense and sustained human effort. In this report, we provide a classification of important web resources for MS-based proteomics and present rating of these web resources, based on whether raw data are stored, whether data submission is supported,and whether data analysis pipelines are provided. These web resources are important for biologists involved in proteomics research.展开更多
基金supported by the National Science and Technology Major Projects(2018ZX09711003).
文摘The COVID-19 pandemic has emerged as a global health emergency due to its association with severe pneumonia and relative high mortality.However,the molecular characteristics and pathological features underlying COVID-19 pneumonia remain largely unknown.To characterize molecular mechanisms underlying COVID-19 pathogenesis in the lung tissue using a proteomic approach,fresh lung tissues were obtained from newly deceased patients with COVID-19 pneumonia.After virus inactivation,a quantitative proteomic approach combined with bioinformatics analysis was used to detect proteomic changes in the SARS-CoV-2-infected lung tissues.We identified significant differentially expressed proteins involved in a variety of fundamental biological processes including cellular metabolism,blood coagulation,immune response,angiogenesis,and cell microenvironment regulation.Several inflammatory factors were upregulated,which was possibly caused by the activation of NF-κB signaling.Extensive dysregulation of the lung proteome in response to SARS-CoV-2 infection was discovered.Our results systematically outlined the molecular pathological features in terms of the lung response to SARS-CoV-2 infection,and provided the scientific basis for the therapeutic target that is urgently needed to control the COVID-19 pandemic.
基金This work was supported by the National Natural Science Foundation of China(No.82173449)the Beijing Nature Science Foundation(No.7182127),the Nonprofit Central Research Institute Fund of Chinese Academy of Medical Sciences(No.2019XK320024)+1 种基金the National Key Research and Development Program of China(No.2016YFC0901500)the Medical and Health Science and Technology Innovation Project of Chinese Academy of Medical Sciences(2019-I2M-1-001).
文摘Dear editor,Mycosis fungoides(MF),the most common subtype of cutaneous T cell lymphoma,is a rare disease[1,2].Patients with early-stageMF have a 5-year overall survival between 88%and 100%[3].In patients with advanced MF,the skin may present with tumors and erythroderma,and the median survival of patients with lymph node and visceral metastasis is 13 months[4].Therefore,it is important to achieve an early diagnosis to improve prognosis[5].
基金This work was supported by the National Science and Technology Major Projects(2018ZX09711003)National Key Research and Development Project(2020YFC0841700)Ministry of Science and Technology of P.R.China Plan(2020YFC0844700).
文摘Dear Editor,Almost one-half of COVID-19 patients have symptoms of liver injury.However,the molecular characteristics in the COVID-19 liver remain unknown.Therefore,the need for long-term medication for recovery make it imperative to study the infectivity and pathogenicity of SARS-CoV-2 in the liver.Here,we have identifed signifcant proteomic changes for the first time in the liver of COVID-19 patients.We detected differential expression levels of several proteins that mediate characteristic cellular host responses elicited by SARS-CoV-2 infection in the liver.
基金supported by the Ministry of Science and Technology of China(Grant Nos.2013CB910801,2012AA020201,2012AA020409,and 2014DFB30010)the National Natural Science Foundation of China(Grant Nos.21105121,21475150,and 61303073)Beijing Municipal Natural Science Foundation of China(Grant No.5122013)
文摘With the development of high-resolution and high-throughput mass spectrometry（MS）technology, a large quantum of proteomic data is continually being generated. Collecting and sharing these data are a challenge that requires immense and sustained human effort. In this report, we provide a classification of important web resources for MS-based proteomics and present rating of these web resources, based on whether raw data are stored, whether data submission is supported,and whether data analysis pipelines are provided. These web resources are important for biologists involved in proteomics research.
