Breeding of Anti-diarrhea Gene in Local and Exotic Pig Breeds in Guizhou Province

[Objectives]This study was conducted to breed special pig breeds resistant to diarrhea by using modern biotechnology.[Methods]From Guizhou local breeds,such as Nuogu pigs,Kele pig,Yorkshire pigs and Duroc pigs,190 samples were collected for the analysis of anti-diarrhea gene.[Results]The anti-diarrhea genotype frequency of Kele pigs was 70.00%,which was higher than that of Nuogu pigs(67.37%)and Yorkshire pigs(Yorkshire pigs and Duroc pigs)(50.59%).The favorable anti-diarrhea gene of all Nuogu pigs,Kele pigs,and Yorkshire pigs and Duroc pigs was G,with gene frequencies of 0.7355,0.8368 and 0.8500,respectively,and the frequencies of allele A were 0.2645,0.1632 and 0.1500,respectively.In the process of generation selection,combination selection of GG♂×GG♀,GG♂×GA♀,GA♂×GG♀and GA♂×GA♀was conducted,and GG individuals were selected while gradually phasing out GA and AA individuals.The anti-diarrhea genotypes of 98 pigs in the offspring were tested,and it was found that the frequency of genotype GG was greatly improved,and the frequencies in Nuogu pigs,Kele pigs,Yorkshire pigs and Duroc pigs were increased to 73.91%,81.82%,85.25%and 66.67%respectively,thus forming a special anti-diarrhea breed.[Conclusions]This study provides a basis for selecting excellent breeding pigs,establishing core populations and screening resistance genes in the core populations and their offspring.

Analysis and Utilization of Straw-preserved Frozen Semen from Native and Imported Pig Breeds in Guizhou Province

[Objectives]Straw-preserved frozen semen from native and imported pig breeds and its conception performance were studied to provide a scientific basis for further optimizing pig breeding strategy.[Methods]This study was conducted to systematically evaluate straw-preserved frozen semen produced by the Guizhou Testing Center for Livestock and Poultry Germplasm from 2018 to 2023.We analyzed 206 samples from native Guizhou pig breeds,including Kele,Zongdi Hua,and Nuogu pigs,as well as 363 samples from imported and bred pig breeds,such as Duroc,Large Yorkshire,Landrace,Topigs,and French Yorkshire,among others.Additionally,we assessed the conception performance of more than 300 heads across 10 batches,comprising both native and imported and bred pig breeds,following deep insemination.[Results]Significant differences were identified in sperm motility and abnormal sperm percentage(ABSP)among native breeds(P<0.05),as well as in number of progressively motile sperm(NPMS)and ABSP among imported and bred breeds(P<0.05).The average semen dose for both native and imported/bred pigs was 0.47 ml,with a standard deviation of 0.01 ml.Sperm motility ranged from 41.5%to 58%in native breeds and 50.4%to 61.7%in imported and bred breeds.The NPMS ranged from 144 to 177 million in native breeds and 149 to 177 million in imported and bred breeds.ABSPs remained within acceptable limits,ranging from 6.6%to 16.8%for native breeds and 6.1%to 18.1%for imported and bred breeds.The imported and bred pigs outperformed the native breeds in terms of sperm motility(50.42%to 69.58%vs.41.63%to 48.37%),farrowing rate(71.13%to 86.70%vs.57.15%to 74.35%),and litter size(7.74 to 10.30 vs.6.39 to 7.45).[Conclusions]These findings suggest that long-term selective breeding and domestication of native Guizhou pigs are necessary to enhance their reproductive performance.

微分博弈下的建筑碳减排研究

随着低碳经济和环境意识的提高,相当多的学者关注并致力于减少建筑供应链内的碳排放。与此同时,消费者在建筑市场上对低碳建筑产品的偏好正在增加,放大了这些产品的潜在效益。本研究采用微分博弈模型研究建筑供应链成员之间的碳减排均衡策略,通过比较和分析集中和分散决策两种策略下的最优反馈平衡策略,可以得到,相对于分散决策,集中决策更有利于建筑碳减排。建筑碳减排的潜在收益越高,就越有利于降低建筑企业的碳排放,对实际碳排放控制有一定指导意义。